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Some biological properties of three standard strains of rotavirus (Wa strains of human rotavirus, NCDV strain of bovine rotavirus and SA_(11) strain of monkey rotavirus) were studied. It has been found that cultural characteritics in MA_(104) cell are similar. The characteristic structures of these ..., Abstract
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A method has been developed to purify HFRS virus structural protein. HFRS virus was first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain tissue of suckling mice infected with HFRS virus strain 76-118 or strain Chen, and the roughly purified virus was then ..., Abstract
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Japanese encephalitis virus SA14 strain(JEV) precipitated by 9% PEG 6000 from cultural medium of C_3/36 cells has been purified by centrifugation through a 20% and 60% sucrose discontinuous gradient. The polypeptides of JEV-SA14 strain were analysed in a discontinuous SDS-PAGE silver staining ..., Abstract
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The capacity of three cell lines transformed by HSV-2 to induce tumours in nude mice was studied. The results indicate: (1) The transformed cell lines of the 29-30th passage were oncogenic in nude mice with 100% positive rate. The other lines of the 47th passage was also oncogenic with 66% ..., Abstract
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A simple plaque assay method for rabies virus has been studied and established. The CTN-2 rabies virus strain was used for testing and BHK-21 cell line was applied for virus growing. The BHK-21 cell suspension in wells of plastic plates were infected with rabies virus and kept in CO_2 34℃ for 8—9 ..., Abstract
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An assay for herpes simplex virus type 1 and 2 induced deoxythymidine (dTK) was described by using ~(125)Ⅰ-UdR as substrate. The assay was also used for detecting the sensitivity of HSV-1 and HSV-2 induced dTK to DHPG. The result showed that sensitivity of viral dTK to DHPG was higher than HeLa ..., Abstract
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In order to investigate the carcinogenic effects of Sapium sebiferum (SS) and Wisktroemia indica (WI), we used the animal model of HSV-2 induced cervical cancer on mouse. The mouse were divided into six groups and the cancer percentage of each group as follows: HSV-2+0.9%NaCl: 13.0%; HSV-2+SS: ..., Abstract
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The nucleotide sequences of 3 end region of some plant viral RNAs(KYMV, CYVV, OYMV, APLV, and EMV) are determined with direct chemical method by Peattie(1970). The possible secondary structures at 3 end of the RNAs have been presented on the basis of nucleotide sequences, which are folded according ..., Abstract
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We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension ..., Abstract
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A human lymphocyte cell line was inoculated with Chen, L99, and SR11 strains of EHF virus respectively. Cytopathic effects(CPE) were observed for 5—7 days after inoculation. First of all, the cells granulated and rounded up, then cell degeneration occured rapidly. The clustered and clumed cells ..., Abstract
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In order to determine whether IFN treatment could make the cellular immune system more effective to against HSV-2 infection and develop a simple techniqe to quantitate the spread of HSV-2 infection in vitro, C57BL/6J mice were infected intravaginally with HSV-2 and treated witha recombinant mouse ..., Abstract
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We have collected an isolate of turnip mosaic virus which infected Chincse cabbage(Brassica pekinensis) and radish(Raphanus sativus), and the isolate was identified by the plant virus researching group in Shenyang Agri. Univ. The pure isolate was propagated in netted green house in the University. ..., Abstract
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Novobiocin, Coumermycin A, Oxolinic acid, Nalidixic acid, ethidium bromide and ATP analogue γ-S-ATP inhibited the DNA packaging of bacteriophage Φ29 in vitro. Besides ATP analogue, the concentration of the chemicals for 50% inhibition were 5, 90, 167, 380 and 1.3μg per ml, respectively. The ..., Abstract
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Capsomer is a morphological subunit of virus. It was regularly arranged in an icosahedral capsid. The numbers of capsomers(N) are defined by the triangulation numbers(T). N=10T+2, T=pf~2, p=h~2+hk+k~2, where f is an integer and p is the class number, h and K are any pairs of integers without common ..., Abstract
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