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Issue of Virologica Sinica
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Review
Investigation into the role of human eytomegalovirus (HCMV) in the development of AIDS/KS was carried out by nucleic acid hybridization analyses of both human KS biopsy and peripheral blood lymphocyte (PBL) specimens for the presence of HCMV transforming mtrⅡ and mtrⅢ DNA sequences. In one set of ..., Abstract
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A cloned Hepatitis B virus (HBV) ayw subtype genome DNA has been inserted into EcoRI site of the plasmid pBR322. The 5 end of the inserted fragment, which was confirmed in positive recombinants by molecular hybridization and restriction digestion closes to BamHI site in the plasmid. We have ..., Abstract
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Review
Detection of the third and fourth components of complement (C3 and C4) and a sequential observation were carried out in 96 epidemic hemorrhagic fever (EHF) patients. Data obtained showed the mean value of C3 and C4 in EHF patients were higher than those of normal controls, while the degree of ..., Abstract
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Review
In this paper, we have modified the HA and HI test for EHF. EHFVHAN was made by Tween_(80)-ether method as well as by sucrose-acetone method. Higher HA titer of EHFV-HAN was obtained by Tween_(80)-ether method. The pigeon crythrocytc was most sensitive to the EHFV-HAN among 6 sorts of red blood ..., Abstract
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K_(562) cells were challenged with HSV-Ⅱ virus and then cultured for successive reproduetion. In the supernatants of the culture no virus was tested in the 2nd and 3nd generations, but in the 4th generation the virus titer was found to be around 10~3TCD_(50). The culture wes carried on smoothly ..., Abstract
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Review
A procedure for obtaining pyrochromatograms of 11 NPV from 6 species insect was described. The differences among the virulanees of same subgroup ould be clearly distinguished through analysis of fingerprinting. There is specificity on both the shape of peak of NPV from 11 strains and peak height ..., Abstract
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A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of ..., Abstract
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Review
Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of ..., Abstract
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Viroid is small and exists less in host, which is difficult to purify. General method of purification is phenol extract, remove carbohydrate, dialysis, LiCl partition, DNase degradation and cellulose chromatography. The procedure is complicated, recovery ratio is less and the pure sample which is ..., Abstract
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Review
The reactions of seven monoclonal antibodies specific for Shanghai Isolate of Ribgrass Mosaic Virus (RMVsh) with virion and its coat protein by two different ELISA methods were compared. The results showed that the properties of reaction of some monoclonal antibodies in two different ELISA methods ..., Abstract
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Review
In the present paper, it is reported that the results of phage adsorption rate constant (ARC) on stationary phasic bacteria, and 50% phages inhibiton (PhI_(50) of LPS μg/ml was estimated, and there is an attempt to analyse the loci and the number of phage receptor sites existed on cell wall of ..., Abstract
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Review
Porcine spleen cells induced with Newcastle disease virus F strain could produce an antiviral substance. The substance was sensitive to trypsin and did not penetrate the dialyser. These indicated that it was characteristic of an Interferon. The antiviral activity of crude porcine spleen cell ..., Abstract
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