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Issue of Virologica Sinica
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Review
Review
Review
12 McAbs to several strains of poliovirus Type 2 and 3 were selected by IF and NT, 9 McAbs had both neutralizing and immunofluorescence activity,3 McAbs were uniquely with IF staining activity. All the 12 McAbs with immunofluorescence activity reacted with poliovirus Type 2 and 3(F-McAbs) and were ..., Abstract
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Review
Serum cytomegalovirus(HCMV) IgM, IgA antibodies were detected in 179 newly diagnosed patients with haematological disorders by ELISA. The positive rates of HCMV-IgM, HCMV-IgA were 11.17% and 11.73% respectively, much higher than that of comparative group. The results showed that the patients with ..., Abstract
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Review
The morphology of koilocyte was observed by light and electronic microscopes, a series of clinical cervical lesions were studied in the presence of human papilloma virus (HPV)DNA and antigens by molecular hybridization and PAP staining method. Having analysed the correlation of the detection of HPV ..., Abstract
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Review
This paper presents the research results of rotavirus(RV)in group A and adenovirus(ADV)in fecal specimens collected from infants with acute gastroenteritis in Zhaotong, Yunnan from October 1982 to December 1985.By means of PAGE, EM and ELISA, 309 fecal specimens of 139 cases were detected with ..., Abstract
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Review
40 serum samples were used to study the relation of Pre-S_1 and pre-S_2 proteins to polymerized human serum albumin receptor (PHSAR). Our results suggested that pre-S_2 may be the main binding site for PHSA, but pre-S_1 may also possess the activity to bind PHSA. Pre-S_1, pre-S_2 and PHSAR may be ..., Abstract
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Review
We first utilized infected mouse brains and lungs to prepare HFRSV specific antigens by treatment with Tween 80-ether. This procedure was very economical and easy. The T-E antigens was a more sensitive, uniform reagent for the RPHI or ELISA and non-multiplication. It also showed an increased ..., Abstract
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Review
We detected viruses in environmental sludge and found that the virus content of sludge was higher than that of water. We simulated natural environment in laboratory to study the distribution and survival of poliovirus 1 in water and in sludge. The results showed that in comparison with water the ..., Abstract
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Review
Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal ..., Abstract
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Review
A soil-borne virus disease on wheat occured in Zhejiang, Jiangsu and Sichuan provinces in China was transmitted by Polymyxa graminis, and its only known host is wheat (Triticum aestivum), most wheat cultivars can be infected by growing in infective soil with chlorotic to necrosis spindle shaped ..., Abstract
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Review
Plasmid N_3 inhibits inductive development in λ lysogen. It makes λ yield lower 10~(-5)—10~(-6) times than that of the contiol. Plasmid N_3 inhibits λ lysogenic development pathway on λ infection. Tire inhibition mechanism of λ development by plasmid N_3 is supposed that the polymerization ..., Abstract
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Review
In this paper some properties of the original strain of the RHDV-A_3R-3 (separated from Wu Xi, China 1984) was reported. The purified virions was stained with 2% PTA and examined by electromicroscopic observation. The results revealed that the virus particles have nonenveloped capsidand exhibited ..., Abstract
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Review
The antigenic relationship between interferon prepared in porcine spleen cells (PS) and human interferon was investigated. The result showed that the PsIFN can be neutralized not only by anti-HuIFN-α sera but also by anti-HuIFN-γ, sera, the ability of neutralization of anti-HuIFN-α was higher ..., Abstract
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Review
This paper reports the results of experimental research and application of polyacrylamide gel(PAG) debris absorption and elution method for Newcastle Disease Virus (NDV) and Human Rotavirus(HRV) concentration. By treating once and twice with PAG, the volume of diluted NDV infected allantoic fluids ..., Abstract
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This paper reports on the observation of treatment with interferon in 33 cases with herpes zoster caused by varicella-zoster virus, the patients with age range from 40 to 78 (over sixty years old was 50 percent) divided into treatmeat group with interferon and control group, Through treatment our ..., Abstract
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Review
A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the ..., Abstract
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Review
A sphere-shaped virus has been found from the diseased plant of primula. Fifteen species plants of seven families were infected, but other three of two families tested were not.The virus purified by PEG(M.W.6000)precipitation and differential centrifugation is found to have a diameter of the ..., Abstract
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Review
The results of our experimental research indicated that epidemic hemorrhagic feve virus (EHFV) couses cell to cell fusion under acidic conditions. Maximum fusions was obtained at pH5.0—6.0. The EHFV-infeeted cell was covered with medium overlaid by methylcellulose and thenthe cell culture ..., Abstract
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