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Issue of Virologica Sinica
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Review
Review
Eight monoclonal antibodies(Mc Abs) from various epidemic hemorrhagic fever(EHF) virus have been used for analyzing the epitopes of SA and TE hemagglutinin antigens by hemagglutination inhibition(HI), reversed passive hemmagglutination inhibition(RPHI), indirect ELISA and blocking ELISA.It was ..., Abstract
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Review
55 patients with chronic infection of hepatitis B virus were divided into three groups: the long time treatment group(LTTG) were 22 cases, the short time treatment group(STTG) were 11 cases and the control group were 22 cases.All patients have been follow-up for one year,the serum HBeAG, DNAP and ..., Abstract
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Review
We explored rabies virus adapted to Vero cells making plaque formation promptly.The plaque could be all exposed and easily seen under microscope on 4th day. It was shown that plaque method was more sensitive than mice(i.c) method on the titer of virus and assay neutralizing antibody.Up to now, the ..., Abstract
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Review
This paper reports the DNA restriction endonuclease analysis of 102 strains adenovirus 3(Ad3) and 7(Ad) causing infant pneumonia from 1976 to 1986 in the areas of Changchun. The results showed that 2 genomes of 56 strains Ad7,7b and 7d were identified by using restriction endonuclease BamHl, Bcll ..., Abstract
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Review
In order to obtain the materials of restriction endonuclease analysis of Herpes Simplex Virus(HSV) genomes and select suitable restriction endonuclease(RE) for studying and typing of HSV infection, the genomes of two standard strains of HSV, HSV-1 F strain and HSV-2 G strain have been digested with ..., Abstract
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Review
165 stool samples were collected from infants and young children with acute diarrhea in Shashi from 1987 to 1988.37 strains of rotavirus were detected by RNA-PAGE, among them 3 strains were unusual rotavirus. The virus is morphologically identical to conventional rotavirus and lacks the ..., Abstract
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Review
It was observed that two strains EHF virus which were isolated newly from patients had pathogenicity on suckling mice.The EHF virus particles were found in the infected mice brain under electron microscope.On IF examination viral antigen could be found in brain, lungs, liver, kidneys and other ..., Abstract
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Review
The Ab_2-1 was used to immunize four BALB/c mice and two rabbits. Each mouse recievcd inoculation of 100μg of Ab_2-1 for four times intraperitoneally. Each rabbit was injected with 1 mg of Ab_2-1 for four times subctaneously. All the mice and rabbits immunized with Ab_2-1 produced anti-HBs ..., Abstract
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Review
Trichoplusia ni nuclear polyhedrosis virus(TnNPV) stimulated thymidine kinase(TK) activities in infected Spodoptera frugiperda cells. TK activities both in infected and uninfected cells are similar in their pH, Mg~(++) optima for assays and elution patterns on DEAE-cellulose and Cibacron-blue ..., Abstract
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Review
Six kinds of baculoviruses(BsNPV1, BsNPV2, BtNPV, EpNPV, PrGV, PxGV) are purified by centrifugation using sucrose gradient,Roughly purified granulins and polyhedrins are obtained by having the inclusion body solubilized in dilute alkaline salne after possible alkaline protease activity is ..., Abstract
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Review
Four diseased samples of chrysanthemum showing chlorotic mosaic or green flowers were collected from the Beihai Park and Zizhuyan Park in Beijing in July and December of 1986.Another one showing black necrotic spots on bulb of Hyacinth was from Beijing Animal and Plant Quarantine Service. These ..., Abstract
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Review
Wheat yellow mosaic virus(WYMV) has been occurred on wheat in Shanxi province since 1970s. The virus preparation was purified from naturally infected wheat plants by extracting in 0.5mol/L potassium phosphate buffer pH7.0, clarifying in 1/4 v carbon tetrachloride, precipitating with 6% PEG, 3% NaCl ..., Abstract
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Review
The principal strain of BYDV, as certified, is the Schizaphis graminum et Rhopalosiphum padi strain which is widely distributed at the northern mid-harvest, late harvest winter wheat and spring wheat growing areas in China. While it was specifically transmitted by the prominent vector inset ..., Abstract
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Review
Examination of ultrathin sections with an electron microscope revealed that aggregates of filamentous virus particle were seldom found, which always distr buted near vacuole membrane in mesophyll cells, and sometimes scattered si- ngle virus-like particles were also seen in some yellowing and ..., Abstract
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Review
The grass carps infected by the Fish Reovirus(FRV) were fed in constant temperature water, in which there are no notable differences of the death-rate(P>0.05)from 24℃ to 30℃. However, when the water temperature was cut down to 20℃ and gone up to 33℃the death-rate were marked reduction(P<0.01). ..., Abstract
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Review
This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) ..., Abstract
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