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A semimicro quick monolayer plaque assay of viruses (SQMPAV) without preparing the monolayer in advance was developed. In this assay, the cells and viruses were simultaneously inoculated into the wells of multi-well tissue culture plates. So the attachment of cells and the adsorption of viruses ..., Abstract
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This paper reports the protective effect of human CSF-1 on human fibroblast cultures infected by viruses in vitro, that is, before virus challenge, the treatment of cell cultures with CSF-1 may result in the inhibition of virus replication and virus-induced cytopathy. The challenge viruses included ..., Abstract
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To explore the histopathological features in chronic hepatitis D, 5 out of 120 cases of HBsAg-positive liver samples were found to be intrahepatitic HDAg-positive by direct immunof luorescence and immunoperoxidase assays. Among 5 cases, 3 had active cirrhosis, 2 cases with chronic active hepatitis. ..., Abstract
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A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that ..., Abstract
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Specific IgM from patients with epidemic parotitis ( EP ) was detected by enzyme linked immunosorbent assay (ELISA). The mumps virus antigen was prepared by using mumps virus Ender s strain. The detected IgM antibody was confirmed by 2-EM destruction. Sensitibity of the assay for mumps virus-IgM ..., Abstract
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The extract of Monordica charantia (E. M. c.) was found to have a significant protective effect on Japanese B encephlitis virus infection in vivo. When mice were administered intraperitoneally with a dose of 0.4 mg E. M. c. at 18 hr, 12 hr, 4 hr before subcutaneous challenge with the virus ..., Abstract
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Rabbits were inoculated with EHFV-H 114 strain isolated from urine of the patient with EHF in the acute phase. The duration of viremic stage of rabbits infected with EHFV-H114 was studied by means of immunoflourescent technique and culture assays. Specific flourescence for EHFV antigen was first ..., Abstract
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In this paper chronic hepatitis B and its virus carriers were treated with HB Vaccine and small doses of interferon and thymosin. After one course of interferon group, placenta peptide group and HB-Vaccine group, the negative percentage of HBsAg were 13.7%, 5.9% and 0%, respectively. The changes of ..., Abstract
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This paper reported the research results for biological properties of six strains of Chikungunya virus (CHIKV) isolated from bats, mosquitoes and patients in Yunnan, China, and Ross strain of CHIKV isolated from Africa. These virus strains all could cause illness and death regularly in two to ..., Abstract
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Effect of temperature on the growth dynamics of the nuclear polyhedrosis virus form Ectropis obliqua hypulina (EoNPV) in its host larvae was studied through polyhedra counting, counter immuno-electrophoresis, single immunodiffusion and rocket immuno-electrophoresis tests. The propagation of the ..., Abstract
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By using BsNPV DNA, HSV DNA and λDNA, the paper reported the re suits of the influence of ionic strength, pH value, reaction temperature and time on the labeling process of DNA with radioactive iodine in the presence of thallic chloride.When pH value was 4.8—4.9, ionic strength(Na~+)was 0.1mol/L ..., Abstract
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Gynura aurantiaca plants infected by citrus exocortis viroid (i.e. CEV) were applied in different concentrations of exogennous gibberellin (GA,5ppm, 10ppm, 50ppm, 100ppm, 200ppm), n(?)phthalen (Nal., 100ppm, 300ppm), 5-flurouracil (5-FU, 0.5mg/L, 1mg/L, 2mg/L) solutions respectively. It was found ..., Abstract
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The seed borne virus(TMV) in tomato can be disinfected by 1 day of dry heat treatment at 70℃, or by more than 4 years storage. Soaking the tomato seeds in the 10% of Na_3PO_4 solution for 30 minutes can not disinfect the virus entirely. However, the 3 methods mentioned above were not effective for ..., Abstract
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Bovine herpesvirus type 4 (BHV-4) DNA was extracted from infected MDBK (Bovine kidney) cells using Triton Ⅹ-100 and phenol/chloroform/ether. With shotgun and selective cloning procedures, the viral DNA was cloned into EcoR I, BamH I or Hind III sites of plasmids pBR322 or pUC9 after digestion of ..., Abstract
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Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because ..., Abstract
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To compare relationship between two pre-s proteins and HBV replication HBV DNA in situ hybridization was applied to a group of patients with chronic hepatitis by bio-probe, in conjunction of detection of intrahepatic HBsAg, HBcAg, pre-s1 and pre-s2 antigens. It was found that the positive rate for ..., Abstract
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