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Review
Review
DHBV in duck serum is assayed by α-~(32)P-DHBV DNA probe hybridization. It is found that the rate of DHBV viremia in hybrid from Beijing duck and Cherry valley duck was 5.1%, whereas in Gauyou local ducks was32.7-58.8%.Positive sera are confirmed by EM. The virion of DHBV is characterized. Ducks ..., Abstract
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Review
In this paper, we studied the infeetivity of Coxsackie B_2 Virus(Cox B_2 V) on human and rat myocardial cells (HMC and RMC) and the effect of chromium chloride(CrCl_3) on growth heart cells and infectivity of the virus. Results indicated that HMC is more sensitive to the virus than RMC. Crcl_3 ..., Abstract
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Review
During 1986-1987, two times of sporadic diarrhea in BALB/c suckling mice and an outbreak of diarrhea in BALB/c and NIH were studied in our breeding farm. The pathogenic agent was identified to be murine rotavirus (MRV) by means of EM, PAGE, infant mice inoculation and ABC antigen location in small ..., Abstract
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Review
Synthesized antisense oligodeoxynucleotides, including modified and unmodified, homo- and hetero-forms, were tested for their ability to inhibit mouse SRS virus-induced cellular multiplication, the formations of colonies of SRSV-infected cells and XC syneytia and RT activity. All oligomers were ..., Abstract
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Review
Human cytomegaiovirus(HCMV)DNA in clinical blood and urine specimens of 27 infant patients infected with hepatitis were detected by DNA-DNA dot blot hybridization using ~(32)P and biotin labeled cloned HCMV AD169 DNA fragment. The hybridization results of blood and urine specimens were compared 16 ..., Abstract
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Review
The status of livers and white blood cells in peripheral blood after infected with hepatitis B virus were studied by Southern blot technique. The positive rate was 44% in white blood cells and 46% in the livers, respectively. There was no significant different beween them. According to speed of ..., Abstract
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Review
The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV ..., Abstract
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Review
Bean yellow mosaic virus (BYMV), potato virus M(PVM) and oat mosaic virus (OMV)were rapidly detected in infected leaf extracts using immunosorbent electron microscopy. The suitable dilution and time for coating the three antisera in carbon coated grids were 1000 and 1h at room temperature. The ..., Abstract
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Review
In present paper, some biophysical and biochemical characteristies of the nucleic acid in the original strain of the Rabbit Hemerrhagic Disease Virus (RHDV)-A_2R-3(separeted from Wuxi, China. 1984) have been studied. By using Feulgen assay, Diphenylamine reaction test and nucleate digestion ..., Abstract
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The presence of any pathogenic viruses in tap water has always been considered a potential public health concern. This study reports the presence levels of viruses and indicator bacteria in the water body of East Lake and treated tap water. During a year s work,indicator bacteria and viral analysis ..., Abstract
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Review
This paper presents a new technique for nucleic acid electron microscopy, i.e, a technique using electrostatic field to cause electrophoretic motion. With water as mediam, the negatively charged nucleic acid molecules will move along the direction of an electrostatic field of electrode distance ..., Abstract
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Review
Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75% formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of ..., Abstract
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Review
This paper reports the experimental studies on propagation of HAV in the human mononuclear leukocytes in vitro. The mononuelear leukocytes were separated from the peripheral blood of health person and the umbilical blood of fetus using Ficoll-Hypaque technique. The NJ-3 strain of HAV was inoclated ..., Abstract
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