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Review
Review
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of human cytomegalovirus (HCMV) in urine samples There were 4 kinds of method of the ELISA and monoclonal antibody directed against 20 or 130 kilodalton envelope structural polypeptides of HCMV was served as a capture ..., Abstract
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Review
In the present study, we report the therapeutic evaluation of 181 patients with chronic active hepatitis(CAH), who were treated with interferon and Chinese drugs(Group Ⅰ), interferon(Grour Ⅱ), heat-clearing and detoxifying Chinese drugs (Group Ⅲ), Chinese and western drugs (Group Ⅳ). ..., Abstract
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Review
In the detecton of epidemic hemmorrhagic fever viral antigen in the autopsy and biopsy tissues by immunocytochemical methods, it was found that the monoclonal antibodies to nuclear antigen could stain antigen well with a rather diffuse pattern in fresh and well fixed material only, Polyclonal ..., Abstract
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Review
We observed that so called mutinucleated giant cells in the EHF patients urine were not occasionally accumulated but fused by epthelial cells. We found that EHF virus antigen which were detected by immunoperoxidase technique in all the fusion cells The results indicated that the fusion cells should ..., Abstract
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Review
The antigens of four EHFV strains from different sources(Chen, H8205, ALC96, R22)purified by sucrose gradient centrifugation incubated with normal human sera in 37℃ for 30 minutes Cross-immunoelectrophoresis was used to detect whether complement C_3 was activated. The results showed that Chen ..., Abstract
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Review
This paper described the quantative eomprison of 3 methods concentrating poliovirus type 1 artificially added into the water samples, The preliminary results showed that the recovery rate of the virus in the water samples was similar (47—49%)among the 3 methods, It was discovered that the ..., Abstract
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Review
Epidemic hemorrhagic fever (EHF) virus strain of Hantan virus isolated from urine of EHF acute stage patient and named H-114 was examined with infected Vero-E6 ultrathin sections by electron microscopy, it was revealed that the morphogenesis of H-114 strain appeared mainly dependent upon different ..., Abstract
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Review
A method using nonradioactive material—alkaline phosphatase to label HBV DNA as probe has been studied and used in clinical experiments to detect the HBV DNA in hepatitis patient s serum. Alkaline phosphatase coupled with PEI (polyethyleneimine) using P-benzoquine as cross-linking reagent. The ..., Abstract
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Review
Restriction mapping of HPV6BV cloned from Chinese women with 14 enzymes was established and compared with that of HPV6b from West Germany and different subtypes of HPV6 reported by foreign reseaehers to find the differences., Abstract
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Review
Two HFRS virus glycoproteins with molecular weight of 78K and 57K were purified by immunoaffinity chromatography with preparative SDS-PAGE from virus-infected cells Purified 78K and 57K glycoproteins all showed only one band on SDS-PAGE and have antigenieity and immunogenicity, The results of ..., Abstract
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Review
A rapid method, micronucleus assay which proposed in the scoring of chromosomal aberrations has been dealt with the safety evaluation of insect viruses of tea pests(Biston marginata NPV, Euproctis pseudoconspersa NPV and Buzura suppressaria NPV)in mice. By scoring the frequency of micronuclei in ..., Abstract
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Review
We isolated a strain of Vu NPV. The present study primarily detailed some changes during the virus infection in larval fat body cells, five days after infection, cytopathological alteration appeared,and the virus has distinguished special structure and process of replication At early stage of ..., Abstract
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Review
Virus transmission was conducted through inoculation of aphids from the same virus carrying individuals with BYDV which was one insect per seeding. The result showed that the duplex immune eleetrophoresis showed positive reaction coinciding with the disease incidence induced by aphids fed with ..., Abstract
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Review
A method for the analysis of monosaccharides of granulosis virus by gas chromatography were described in this paper. The main components of monosaccharide chromatogram were identifed, showing that the componets of virus monosaccharides were composed of rhamnose, ribose, xylose, mannose, glucose and ..., Abstract
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Review
A Capsicum Mosaic Virus(CaMV) was studied on some biochemical charaeteristics. UV-absorbance curve of viral protein at 280nm is maximum, at 260nm is minimum. The molecular weight of coat protein is 17500, consisting of 148 amino acid residues. The purified nucleic acid is confirmed as linear RNA ..., Abstract
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Review
Seed transmission incidences of the inoculated plants in the greenhouse and the infected plants in the fields were investigated in the greenhouse, some of them were detected with ELISA methods. The highest seed transmission incidence was 29.60% and the lowest was 1.04% among the tested soybean ..., Abstract
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Review
In our experimental studies, 6 strains of EHFV were inoculated into the Wish cell cultures. We found that EHFV could propagate in this cell line and after several passages, viral titers can reach to 10~(4-5) TCID50/ml. After incubation no cell cytopathic effect could be observed in the Wish cell ..., Abstract
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Review
It is difficult to recover DNA small fragment in moleeular biology. By using some procedures of digestion, electrophoresis and ultracentrifugation etc, an efficient method to recover 51bp polylinker is introduced in the paper., Abstract
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