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Issue of Virologica Sinica
Article List:
Review
Review
Review
Up to present, few was reported about the natural infection of murineeytomegalovirus(MCMV) in laboratory mice. Based on the experimental infection of MCMV, we made a survey on the natural infection of MCMV in 10 common used strains of mice It was found that of 334 samples of submaxllary gland ..., Abstract
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Review
Effect of Astragalus Membranaceus(AM) in lymphocyte subsets in an animal model of viral myocarditis were investigated by antigen specific cytotoxicity assay, The percentage of Thyl+cell in the peripheral blood and spleen was significantly higher in AM treated mice than in normal saline(NS) treated ..., Abstract
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Review
Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of ..., Abstract
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Review
The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means ..., Abstract
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Review
Half and one year after the epidemic of acute hemorrhagic conjunctivitis(AHC) caused by eoxsackievirus A24 variant(CA24v) in the autumn of 1988, 423 sera were collected and the neutralization antibody(NA) and specific IgM against CA24v were tested in microplate with Hela cells. The positive rate of ..., Abstract
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Review
A sensitive and simplified method of ~(125)I-labeled peptide mapping was used to estimate the rates of the haemagglutinin(HA) mutation of 5 strains of influenza Al viruses and 5 strains of influenza A3 viruses. It could be calculated that the average rates of HA mutation for these two subtypes of ..., Abstract
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Review
Serotypic classification of 14 HFRS virus isolates from different sources were studied using plaque reduction tests(PRNT) sera of rabbits inoculated with different HFRS viruses were tested against reference type-1 Hantaan virus and type-2 Seoul virus. The results indicated that except one ..., Abstract
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Review
A RNA hyhridization assay for hepatitis D virus in serum and liver specimens, from human and infected chimpanzee experimentally, was described using a nonradioactive Digoxigenin labelled RNA probe, and was compared with the ~(32)P labelled RNA probe. Findings show that the sensitivity and ..., Abstract
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Review
In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the ..., Abstract
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Review
In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera ..., Abstract
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Review
The BALB/C mice were immunized with HeLa cells infected with respiratory syncytial virus(RSV, Wuhan strain). The immunized spleen cells were fused with SP2/0 myeloma cells which were obtained from the SP2/0 solid tumor grown in BALB/C mice. Thirteen hybridoma cell lines secreting antiRSV monoclonal ..., Abstract
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Review
Recently, we isolated a non-occluded virus from spontaneous mortal Periplaneta fuliginosa(Serville). The virus suspension was mixed in sterile food to feed healthy P. fuliginosa nymphs(of 154—169 days). The percent mortality of the nymphs which died with infected virus was over 98%. Under ..., Abstract
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Review
It had been manifested that corn was readily infected by wheat BYDV The symptoms were similar to those of the wheat The most effective veetor aphid species was R. padi, while M. avena, S. graminnm and R. maidis were also among the vectors. The isolates belongs to the main strain of BYDV, eg. S. ..., Abstract
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Review
In Zhejiang, Jiangsu and other provinces, 5 varieties of the garlic crops are totally diseased. The virus disease frequently causes degeneration of viruliferousness, loss of yields and reduction of tolerant to store. Symptoms due to the infection of virus are chlorotic mottling between the veins ..., Abstract
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Review
An immuno-colloidal gold labelling procedure was developed to localize barley mild mosaic virus(BaMMV) in thin sections of barley leaf and root tissues embedded in L. R. White resin, The optimal procedure for specific labelling of virus with a minimum of background labelling and economical use of ..., Abstract
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Review
During 1986—1989, 111 isolates of cucumber mosaic virus were obtained from tomato fields in Shandong province According to the host ranges and symptoms on different hosts, three pathogen strains of CMV were established as tomato fern leaf strain(CMV-ToF), tomato mosaic strain(CMV-ToM) and tomato ..., Abstract
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Review
Three strains of retrovius, which can give positive results both by ELISA and immunofluoreseenee assay(IFA) with SRV-1 newly have been isolated from Rhesus monkeys(Macaca mulatta) catehed frim three areas of China. It have been confirmed by Western Blot that their seroantibodies response with SRV-1 ..., Abstract
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Review
With serotype B271 of Corynebacterium crenatum phage as a model, we established a method for studying the survival of aerosol of fine biological particles such as virus. The paper discussed some important techniques for the study of survival of viral aerosols, including the viral resistance to ..., Abstract
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Review
This paper reports the derivation and calculation of several formulas for triangulation number and eapsomer number, The formulas are P=h~2+hk+k~2, T=pf~2, C=10T+2 and C=10p(n-1)~2+2. And the correlations among the formulas and the meaning of the parameters are given., Abstract
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