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Issue of Virologica Sinica
Article List:
Review
Review
Using polyclonal antibody (PAb)and monoclonal antibodies(MAbs) against epidemic hemorrhagic fever virus (EHFV), the viral antigens in fresh and long-term storage EHF autopsy tissues were studied comparatively with more sensitive multi-layer PAP and PAP combined with ABC (PAP+ABC) techniques. The ..., Abstract
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Review
人乳头瘤病毒16型(HPV16)含有两个晚期开放读码框(L_1ORF和L_2ORF),L_1ORF编码主要衣壳蛋白,我们用质粒pHPV16、p16L_2BX_5和pATH,采用基因重组技术,制备了含有HPV16个长L_1ORF序列的基因克隆p16L_1BN(5071—253),它能在大肠杆菌中有效表达,产生分子量约90KD的含有E. coli trpE的融合蛋白。Western ..., Abstract
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Review
In order to probe the relatignship between human papillonavirus type 16 and head-neck tumors, using fresh tissue and paraffin-embeded tissue sections preparing DNA to detect HPV16 DNA in head and neck tumor, by PCR and hybridization technique. The results showed that HPV16 DNA sequences were ..., Abstract
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Review
In the present paper. the primers PG01 and PG02 were synthesized in which the EcoRI and BamHI recognition sites as well as the initiation and termination codons were designed for convenience of subsequent cloning and expression. Micrograms of target gene sequence was produced by 30 rounds of ..., Abstract
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Review
The immune persistance of three batches of domestic Hepatitis B Vaccine at a dose of 10μg were studied for four years. The results showed that in the same age group there was no difference in immune persistance by sex. The critical protective rate and effective protective rate were higher in the ..., Abstract
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Review
Methods such as immunodiffusion. counter-immunoelectrophoresis. enzyme-linked immunosorbent assay (ELISA) and solidphase immune electron microscopic(SPIEM) technique were used to study the antigenicity of EpNPV and relationships between EpNPV and other ten kinds of NPV Results indicated that the ..., Abstract
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Review
Effect of the nuclear polyhedrosis virus from Ectropis oblioua hypulina Wehrli (EoNPV) on the reproduction of tea geometrid under high temperature condition in July and August was reported. The results indicated: The development rate of ovaries in female pupae or adults survived from the viral ..., Abstract
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Review
This thesis reported the following infective charaeters of Buzura suppressatia nuclear polyhedrosis virus (BsNPV) in Bs484 cell line: 1. The viral infection rate was the highest when the virus was inoculated in Bs484 cells had been subeultured for 3—4 days. 2. The viral inocuiating quantities ..., Abstract
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Review
Cytopathology of mjdgut cells of Euproctis pseudoconspersa infected with a nuclear polyhedrosis virus was studied by transmission electron microscope, seannig electron microscope DMSO cracking method, ultramicrotome, energy dispersive X-ray microanalysis and negative staining method. Between ..., Abstract
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Review
The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. ..., Abstract
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Review
In 1990—1991. from the fields in near- and-far suburb of nine counties or municipalities in Siehuan province, 492 accessions of Brassica juncea plant showing viral disease symptom were collected both in the initial and the prevalent period of the disease and were subjected to indirect ELISA tests ..., Abstract
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Review
According to Flor s "Gene to Gene" theory and Omaro and Tomaru s method to distinguish CMV strains, we studied "strains of gene type" and "strains of pathogenetic type" 5 varities were selected as differential variety spectrum from 373 sweet or hot pepper, They were numbered LS-8501 (HR) ..., Abstract
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Review
The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the ..., Abstract
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Review
In this paper four different strains of RHDV were purified by two phases of polyethylene glyeol-dextran sodium sulfate andcentrifugation, then virions in highly pure form were obtained by using Sepharose 4B chromatography. The recovery rate of virus preparation was about 70 pereent. Serological ..., Abstract
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Review
In this paper the characteristics of replication of RHDV in the RL and RK cells have been studied by using synchronous infective method. The cytopathology effect can be observed at 48—96 hrs after viruses infection. HA titer of viral increased 5—10 times as high as those HA titers of viruses ..., Abstract
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Review
Antigenic relationship between rabbit hemorrhagic disease virus (RHDV) and six parvoviruses was determined with indirect enzyme-linked immunosorbent assay (indirect-ELISA), enzyme-linked immunosorbent inhibition assay (ELISIA) and hemagglutination inhibition (HI) test. The results by indirectELISA ..., Abstract
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Review
The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the ..., Abstract
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Review
A new detection method has been developed by a new technique of polymerase chaiu reaction (PCR). According to the sequence of PSTVd, two primers were designed and synthesized with automated DNA synthesizer. The cDNA was synthesized by reverse transcription of PSTVd RNA, the cDNA was then amplified ..., Abstract
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