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Issue of Virologica Sinica
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Review
Review
Review
In 1989, 3 strains of Yunnan RSSE virus were isolated from 2 groups of Ixodes Ovatus (T56, T57) and blood of a female patient with fever of unknown infected with YHV, T56v, T57v by intracerebral, intraperitoneal, nasal cavity and subcutaneous inoculation respectively.The incubation period is 3—4 ..., Abstract
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Review
Using a polyhedrin-positive transfer plasmid and a nonoccluded (OCC-) parental recombinant, an occluded (OCC~+)recombinant baculovirusdesignated TnNPV-HBs85-OCC~+ was successfully constructed which contained the gene coding for the hepatitis B virus surface antigen under the dual control of the ..., Abstract
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Review
In 1988, Russian spring-summer encephalitis viruses were isolated from Ixodes ovatus and the patient at Gaoligoug Mountain natural focus in Yunnan In 1990, 15 strains of viruses were isolated from R.confucian, A. sglvaticus, R. eha ninus, E. eleusis, N. bramha, N. sinensis, R.nitidus and ..., Abstract
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Review
No side effects were found locally and generally in 25 volunteers when the secordary immunization(one injecton) with inactivated EHF vaccine was done at a year or a year and half after primary immunization, The EHF virus antibodies were detected by PRNT in these volunteers who were negative of ..., Abstract
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Review
A portion of dengue 2 virus strain Haina 98 (DEN2 HN98)envelope (E1-305 nt) gene has been isolated by reverse transcription and polymerase chain reaction(RT-PCR). The plasmid pDEⅡ305 was constructed by blunt ligation of the PCR products of 305 bp fragment with SmalⅠdigested pUC18. The DEN2 E305 ..., Abstract
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Review
An enzyme linked immunosorbent assay for detecting antiidiotype antibodies against anti-HBs-containing immune complexes (antiHBs-Ab_2IC)in hepatitis B patients has been established, which was based on precipitating IG by 35.0g/L and 17.5g/L polyethyene glycol(PEG, MW 6000) and separating IC by ..., Abstract
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Review
Malacosoma neustria testacea nuclear polyhedrosis virus(MntNPV)is a kind of multi-embeded baculovirus, Scanning EM shows that MntNPV inclusion bodies are irreguIar polyhedra with different sizes The average diameter of polyhedra is 1.3μm. Virions have a mean size of 366×90nm, The virus genome is a ..., Abstract
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Review
In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit ..., Abstract
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A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. The synthesized eDNA was cloned in plasmid pUC19 in JM103. The ..., Abstract
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Review
Studies on paramyxoviruses and myxoviruses have shown that the viral pathogenesis is related to the cleavibility of the viral spike protein, in turn, cleavibility of the spike protein is determined by the amino acid sequence of the connecting peptide of the spike protein This paper intended to ..., Abstract
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Nineteen monoelonal antibodies (McAb) specific for Newcastle Disease Virus (NDV)was produced and used for the reactivity detection to 14 international reference NDV strains and 16 domestic NDV isolates by indirect immunofluorescent assay (IIF) . According to the results, the 30 NDV strains was ..., Abstract
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This paper reports on thermal stability test of three Chinese oral polio vaccines (OPV), The result showed that the time of test temperature to keep effective dose at 4℃ for 4 months,10℃ for 2 months.22℃ for 7 days 34℃ for 2 days and 36℃ for 1 day, It is discovered that The thermal stability ..., Abstract
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The main alternations of cell infected with DaMV were the formation ofspherical or ovoid intracellular inclusion bodies, c.1.6—3.1μm /tin in diameter, which were surrounded by more ribosomes, Golgi bodies and vesicles, and the increase of peroxisomcs The chloroplasts and mitoehondrions of the ..., Abstract
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Review
We used the recombinant clone eontaing RSV-CP gene as material. After being digested with restriction endonueleases, RSV-eDNA fragments were subcloned into M13 rap18, 19. The CP gene sequence was determined by dideoxynucleotide chain termination method and the result revealed that RSVCP gene ..., Abstract
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