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Issue of Virologica Sinica
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Review
Review
In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP ..., Abstract
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Review
The EHF RNA and its G2 antigen were detected by in situ hybridization and immunohistoche- mistry on liver biopsy specimens of 25 cases of EHF patients among whom 23 are postive. Our results indicated that the degeneration and the single hepatocyte necrosis were due to the EHF virus invading ..., Abstract
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Review
Double-immunoctytochemistry and double-immunoelectron microscopy were applied to investigate the pathogenesis and virus localization in cyclophosphamide-treated adult and suckling BALB/c mice af- ter the infection of hemorrhagic fever with renal syndrome virus. It was found that, after the infec ..., Abstract
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Review
A small hydrophobic polypeptide of 55 amino acids, noted as the 6K protein, could be detected in purified preparations of Sindbis virions (SbV). 6K proteins become associated to the E2 protein after their cynthesis in the endoplasmic reticulum (ER) and during their transport process from ER to the ..., Abstract
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Review
Adult diarrhea rotavirus (ADRV) is known as only one of group B rotavirus that causes human diarrhea. The viral proteins were determined by SDS-PAGE of purified virions from infected feces as follows: VP1 (136K), VP2 (113K), VP3 (92K), VP4 (84K), VP5 (64K), VP6 (47K), VP7(41K). Western blot ..., Abstract
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Review
The viral antigens in 16 kinds of 10% formalin-fixed paraffin-embedded tissues of 19 fatal cases due to the death of hemorrhagic fever with renal syndrome (HFRS) were detected and anslyzed by 15 clones of monoclonal antibodies (MAbs) respectively against glycoprotein Ⅱ(G2), nucleocapsid (NP) and ..., Abstract
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Review
Arscs NPV DNA and Ar NPV DNA purified by SDS-phenol method were digested by restriction endonucleases EcoRI and BamHI. Arscs NPV DNA resulted in 5,5 fragments and Ar NPA DNA result ed in 8,7 fragments respectively after analysis on agarose gel electrophoresis. Five of eight fragments of Ar NPV DNA ..., Abstract
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Review
A concentration of 1×10~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was ..., Abstract
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Review
A filemental virus was detected from natural-infected Z. aethiopica showing mosaic and distortion symptoms in Hangzhou,Beijing and Shanghai. The average length of the virus paticles was 745nm and the most distribution was 720-750nm. The virus became strongly decorated with an antiserum of Dasheen ..., Abstract
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Review
Following the successful identification of sesame dwarf necrosis disease, this study focused on the identification of sesame yellow mosaic disease which widely spreaded all over the sesame-grown areas in China with general infecting rate of about 5%. The virus (YMo-I) infected sesame (Sesamum ..., Abstract
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Review
The tests proved cucumber mosaic virus (CMV) could be transmitted by cucumber seed with the rate of 0.20% in seed surface and embryo. Seven and twelve biennital or perennial weed species were infected by CMV through ELISA detection and the necrotic spot reaction. Among them,Seven newly discovered ..., Abstract
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Review
This paper is a study on the result of animal model with HCMV. Rabbits were inoculated with HCMV-AD_(169) strain. The duration of viremic stage of rabbits infected with HCMV was studied by means of immunoflourescent assays and reisolation of the virus. Specific flourescence for HCMV anti- gen was ..., Abstract
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Review
The expression plasmid pWS-4 containing Rabies virus glycoprotein gene was recombinaed with vaccinia viruses in the presence of Lipofectin~(TM) Reagent by homologous recombination technique in chicken embryonic cells. The Lac gene on the recombinant was used as a selected marker for isolation of TK ..., Abstract
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Review
The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ..., Abstract
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