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Issue of Virologica Sinica
Article List:
Review
Advances in Baculovirus Transpasable Elements, Abstract
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Review
A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. ..., Abstract
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Review
A total of 7.2 kb of full-length genome of hepatitis E virus (HEV)XT-179 strain was cloned as cDNAs that isolated from a HE patient s fecal sample during an epidemic outbreak peak of Sept. 1990 in the East Xinjiang of China, and the complete nucleotide sequence has been determined. This strain have ..., Abstract
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Review
Antisense phosphorothioate oligodeoxynueleotides respectively complementary to initial region of 2.1kb mRNA(oligomer A,14nts)and enhancer I(oligomer B,12nts)of HBV were synthesed by chemi cal synthetic method,and inhibition of these oligomers on expression of HBsAg and HBeAg in cell cul ture system ..., Abstract
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The levels of interleukin-1 and interleukin-2 production in 16 healthy blood donors bone mar row mononuclear cells infected by HBV in vitro and on 16 cases with chronic hepatitis B were simulta neously assayed. The evidence of virus growth is based on the detecion of HBcAg in bone marrow ..., Abstract
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Review
In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. Southern blot showes that RCN has TK gene sequence though it hybridizes week ly with vaccinia virus TK gene. But RCN is resistant to 5-BUdR even after it was plaque purified in ..., Abstract
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This paper described immunization effect and response of two and three injections with 3ml inacti vated EHF vaccine in volunteers. This experiment was derided into three groups. Group 1. Injected three times on day 0,7 and 28(1 ml once);Group 2.Injected twices on day 0 and 28(1.5ml once); Group ..., Abstract
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Review
By detecting specific HSV nucleotide sequences with polymerase chain reaction (PCR), we investigated one hundred of pregnant women and women in childbirth for understanding HSV infection rate in their reproductive organs and the posibility of transmiting HSV to filial generation. The results showed ..., Abstract
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Review
By detecting specific HSV nucleotide sequences with polymerase chain reaction (PCR), we investigated one hundred of pregnant women and women in childbirth for understanding HSV infection rate in their reproductive organs and the posibility of transmiting HSV to filial generation. The results showed ..., Abstract
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Review
SDS-PAG electrophoresis showed that the polyhedron proteins of Leucaia separata nuclear polyhedrosis virus(LsNPV)was consisted of several polypeptides, the major band of MW 32kD was the major structural polypeptides of polyhedra. Amino acid composition of 32kD protein was analyzed after ..., Abstract
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Review
Purified potato virus Y, X, and S were detected by indirect enzyme-linked immunosorbent assay (ELISA) on nitrocellulose membranes. Potato virus Y, X, and S in infected tobacco, sprouts of potato tubers, rose end of dormant tubers were detected respectively. The results indicated that the small ..., Abstract
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Review
A rice virus-like disease broke out in Wenzhou, Zhejiang Province. The infected rice plants became stunt with darking and hardening leaves. Along the veins of sheath, culm and underside of leaves, some waxy swelling could be found, white first, then black brown. When four insects (Laodelphax ..., Abstract
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Review
Five virus free isolates of Polymyxa graminis were purified through sand culture of susceptible wheat cv Yangmai 4 inoculated with selected cystosori from diseased wheat roots which were collected from wheat spindle streak mosaic virus (WSSMV) infected fields. The virus free P. graminis acquired ..., Abstract
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Review
The temperature-inducible expression vector containing the beet necrotic yellow vein virus(BNYVV)isolate NM coat protein gene was constructed and transferred into E. coli DH5 α. High-level expression of the specific protein was achieved by temperature induction. The results of SDSPAGE and ..., Abstract
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Review
Tai MV was routinely detected and diagnosed by ELISA method. The detective sensitivity of the method was 4ng. Fields detective results showed that the soil, the roots tubers of Taizishen, symptomless plants and the surfaces of seeds had serological reactions with Tai MV antiserum. And it was ..., Abstract
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