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Review
Review
epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection ..., Abstract
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Review
his paper reports the detection of HBsAg,HBeAg and HBV DNA in the urinary cells by IFARPHA ELISA and molecular hybridization techniques.10 HBsAg carriers and 89 patients with hepotitis B were exanlined.The results indicated that HBsAg,HBeAg and HBV DNA could be detected inthe urinary cells.It also ..., Abstract
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Review
he infection of HPV is the most frequent among the viruses tropic to the epithelium of cervix.The different HPV types possess various infective location and the abilities of etiology,for example,HPV type 6B,type 11 were correlated with the cervical non-tumor lesions,otherwise,the HPV type16 and ..., Abstract
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Review
uinolones were studied for their ability to inhibit hepatitis B virus DNA replicatiun,HBsAg andHBeAg exporssion in a HBV-transfeeted eell line(2.2.15 cell).The survival cell rate was determinedby MTT assay.The result shows that ID50(the drug concentration that inhibits HBsAg or HBeAg secretion by ..., Abstract
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Review
rimer selection is important for the detection of HCV RNA with RT-nested PCR method Com.pered to primers from 5'-UTR gene,the primere from NS5 gene had also high sensitivity and specifityin the detection of HCV RNA.Sixty-nine of 131 cases of anti-HCV Ab positive and 8 of 22 cases ofanti-HCV Ab ..., Abstract
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Review
ixty-one children patients with neonatal hepatitis were investigated for human cytomesalovirus(HCMV)by single PCR and nested double PCR.of the 61 patients,33(54.1%)were positive forHCMV by single PCR,whereas 47(77.0%)were positive by nested double PCR.This showed that nest-ed double PCR is more ..., Abstract
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Review
he major eapeid protein gene(vp39)of Autographa californica nuclear polyhedrosis virus(AcNPV)was amplified successfully by PCR technique and cloned into pBluescript SK(+).With pure AcNPVvp39 gene as a probe,the major capeid protein gene(vp39)of Leucania seperata nuclear polyhedrosisvirus has been ..., Abstract
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Review
he results of longan seedlings with witches'broom disease immersed in tlie solutions of tetracycline-HCl(500,1500ppm for four hrs)and penicillin-GK(5000 ppm for four hrs)showed that thesymptom of the diseased seedlings were unable to be inhibited,which indicated that the disease was notcaused by ..., Abstract
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Review
lethal disease of tomato eecurred severely in the vicinity of Nanjing from 1991 to 1992 wascaused by a viral pathogen.A virus was isolated from the diseased tomato collected from field and.called the isolate TN.It infected 8 families 26 species host plants out of 10 families 39 species inoculated ..., Abstract
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Review
weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was ..., Abstract
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Review
t had been reported that the GCHV(Gtass Carp Hemorrhage Virus)is a new member.in Reoviri-dae and has 11 polypeptides by our laboratory,We did further experiments on the viral polypeptides.The virions(GCHV-875)were purified from the medium of infection culture cells by CsCl density gradient ..., Abstract
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Review
mpligen,a known immunomodulator and interferon inducer.was used alone and in conibination with other antiviral agents to treat ducks congenitally infected with duck hepatitis B virus.Theseantiviral agents included the conventional nucleotide analogue gunciclovir and the prokaryotic DNA gyrase B ..., Abstract
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Review
uring the cultivition of Penaeus orientalis K,outbreak of death or slowing down of growth couldoccurred sometimes.tuge amount of dispersive spherical viruses with diameter around 80nm were observed in the midgut cells of those sick shrimps under electron microscope.Hoat cell organelles,such ..., Abstract
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Review
his paper talculates the length of the 5,3,and 2 fold rotational symmetry axes and the derivesforuiulas from them,they are and where a5,a3,and a2 are the length of the 5,3,and 2 fold axes reslxctively,1 is the distaisee between any two adjacent capsomeres,Tis trtangulation number.The formula of the ..., Abstract
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Review
very best choice of several rnain affecfing factore on the crude purification of PRV was reporteddetailediy in this paper.The advanee purification method of PRV with the Cs SO4 continuous densitygradient supercentrifusation for about 3 hours with 30% sugar cushion was tested for the first ..., Abstract
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