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Issue of Virologica Sinica
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Review
Review
Review
Aedes albopictus and Ae.aegypti mosquitoes were tested by oral infection with four strains of Chikuirgunya(CHIK) virus isolated from Yunnan,China and Africa for their susceptibility and transmissibility.Studies showed that these two species of mosquitoes were susceptible to CHIK virus. Either ..., Abstract
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Review
The ultrastructure of CM_2 cell line,derived from peripheral blood of a patient with nervous syatem disease and the morphology and morphogenesis of its harbored type C virions were reported.Thecell size by electron micrcacopy in thin section was about 7-15 micron in diameter.The pleomorphiccells ..., Abstract
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Review
Infectious mature human immunodeficiency virus type 1 (HIV-1) carruig pre-virus DNA was evidenced by pelpoerase chani reaction(PCR).When the samples were treated with lytic buffer,the result of PCR should be positive;however when PBS was added uistead of lytic buffer,the DNA wasnot detected. The ..., Abstract
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Review
Genome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid ..., Abstract
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Review
Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by ..., Abstract
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Review
n isolate of Chinese squash leaf curl virus(SqLCV-C),which is transmitted by whitefly Bemisia tabaci and caused leaf curl and severe stunting symptoms on Cucurbita moschata and C,pepo,have beenisolated and further confirmed by molecular hybridization using its coat protein gene probe in ..., Abstract
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Review
n identification of a mosaic virtis disease of eggplant which occurs in Shenyang was carried outduring 1991 and 1992.The isolate can be transmitted to some plants by mechanical inoculation,butcannot be transniitted by peach aphid(Myzus pericicae Sulzer,Phopalosiphum pseudobrassicae).Host ranse ..., Abstract
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Review
Comparative studies were made among five CMV isolates cullected from pea(Pisum sativum),hyacinth bean(Dolichus lablab),bean(Phaseolus vulgaris)and red bean(P.angularis),which were namedCMVP_2 CMVP_3,CMVHB,CMVB and CMVRB,respectively.No difference was found in stability insap,aphid ..., Abstract
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Review
From September 1991 to August 1992,leaves,stem barks and roots collected in each monthfrom four-year-old satsuma budling infected with satsuma dwarf virus(SDV) and from four-year-oldsweet orange seedling infected with citrus tristza virus(CTV)were used for detecting SDV and CTVby using ..., Abstract
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Review
sphere-sliaped virus has been isolated from the diseased sugar beet in Nighxia,The virus parti cle was 28nm in diameter.The virus could infect seventeen plants,They belong Cenopodiaceae,Legu minosae,Cucurbitaceae,Solanaceae and Aizoaceae,respectively,There has serological relationship be tween this ..., Abstract
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Review
The present study established and further improved the direct antisen cuating form of indirectELISA(DAC-ELISA)and Dot-ELISA for the detection of papaya rinsspot virus(PRV)in infected tissuses of papaya(Carica papsys L.)or zucchini squash(Cucurbita pepo L.).The results showed if theELISA method to ..., Abstract
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Review
The morphogenesis and antigen localization of infectious canine hepatitis virus(ICHV)in dog kidney cells were studied with electron microscopy and immunogold electron microscopy,Apart from theassembly of ICHV in nuclei,virions were also assembled in cytoplasm of the infected cells.The modeof viral ..., Abstract
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Review
specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both  ̄(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes ..., Abstract
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Review
In isolate of Chinese squash leaf curl virus(SqLCV-C),which is transmitted by whitefly Bemisia tabaci and caused leaf curl and severe stunting symptoms on Cucurbita moschata and C,pepo,have beenisolated and further confirmed by molecular hybridization using its coat protein gene probe in ..., Abstract
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Review
PCR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired ..., Abstract
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Review
Duck hepatitis B virus re plicativc complexe~ (DHBV RCs)wcl~e isol~ted from DI-~V—infected duck liver by chromato~aphy thxou Bio--Oel A 5m followed crose gradient centrifugation.The feIathe endogenous DNA polymcra.se activity of the purified RCs was 100 tim es higher than that of the ..., Abstract
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