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Issue of Virologica Sinica
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Review
Review
Review
Polymerase Chain Reactiun(PCR)was used to detect HPV 6,11,16,18 type specific DNA se-quences in 2l2 clinical cervical samples.The pusitivity uf HPV-DNA was 62.5%(25/40)in patientswith cervical cancer(squamous cell)and 57%(81/142)in chronic cervicitis,markedly higher than20%(6/30)in hedlthy ..., Abstract
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Review
The NS3 gene fragment of HCV was cloned from the serum of hepatitis C patients in Jiangshuprovince by RT-PCR.DNA sequencing shows the cloned NS3 cDNA contains 842bp,NcoI site and sal-I site were added respectively to the upetream and downstream primers.The cDNA was cut with NcoIand SalI,and then ..., Abstract
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Review
This paper reported the antiviral effect in 54 cases of chronic hepatits B,who were serum HBVreplication mark positive.It was divided into two groups:the treatment group and the control group in27 cases respoctively.In the treament group,acyclovir was used according to 25-20mg/kg/d in thefirst ..., Abstract
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Review
our Heliothis NPV isolates(two SNPV:HaS and HzS two MNPVs:HaMl and HaM2)were com-pared on the shape and structure,biological activity,structural polypeptide,restriction pattern andDNA homology.The LD50 values of the four isolates for late second to early third instar H.armigera larvae were ..., Abstract
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Review
CMV isolate(CMVP 1)containing satellite RNA was obtained from pea(Pisum sativum)in Jiangsu province in 1990.Twenty-nine plant species representing nine families were inoculated mechanically with CMVP l and no symptoms or only mild symptoms were found.In Nicotina tabacum-Samsum,CMVP 1 caused Slight ..., Abstract
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Review
The isolate 87-35 was isolated from tomato in Xinjiang.After inoculating to tomato inducing mot-tle,the virus particles were isometric,and about 25nm in diameters,The virus infected 18 speciesplant-Chenopodium quinoa,C.amaranticolor,broad bean and tomato etc.It was transmitted by aphid(Myzus ..., Abstract
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Review
They have isolated garlic mosaic virus(GarMV)from naturally infected garlic plants and synthesized various pattial cDNA fragments using the GarMV genomic RNA as template.The length of thecoat protein(CP)gene was estimated about 0.8 ̄0.9 kb based on the estimated molecular weight ofthe coat protein ..., Abstract
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Review
The cytological effects induced by beet necrotic yellow vein(BNYVV),beet soil-borne(BSBV),peanut clump African sourec(PCV-A),peanut clump Indian suurce(PCV-I)and soil-borne wheat mo-saic viruses(SBWMV)were studied.The four virusee,differed in the structure of membrane accumula-tions and/or the ..., Abstract
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Review
The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of ..., Abstract
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Review
The hybridization assay with digoxigenin(DIG)-labeled probe was developed for detection of in-fectious bursal disease virus(IBDV)and it's semitivity was compared with that of immunodiffusiontest in this experiment.IBDV STC-119 and STC-24 3 cDNA fragments used as probe were preparedfrom recombinant ..., Abstract
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Review
ll 11 segments of GCHV(grass.carp hemorrhage virus)873 dsRNA were isolated and individual-ly retrieved by SDS-PAGE and electric elution.Each viral genome segment in 90%DMSO denaturedby heating at 45℃ for l5 min were translated in the wheat germ tramlation system at 25℃ for 70min.The translation ..., Abstract
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Review
a lot of virogenic stroma,nucleocapsids,envelope and virion is detected in hepatopancreatic andmidgut epithelium cell nucleus of infected shrimp.Virion shapes short bar with blunt circle at twosides.The average size is 250-300×110nm.No inclusion body is deteeted in nucleus.There aresome ..., Abstract
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Review
AVWC-test is a new bio-statistical method which can transform the nonparameter datum into theparameter one to take the statistical analysis according to measurable datum.Some of the scienfific data were selected from the different research papers published in the academic magazine,analyzed ..., Abstract
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Review
The changes of sheep foetal lung cells infected by Ovine Progressive Pneumonia Virus(OPPV)were observed by.indirect immunofluorescence assay(IFA)and with the prepared fluorescence antibody of oppv.The result indicated that the fluorescence reaction initially appeared in 18 hours postinfection,The ..., Abstract
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Review
The toxicity of a strain of Spodoptera tilura nuclear polyhedrosis virus(SINPV)was tested.The 4th instar larvae of Spodoptera titura were used to carryout the bioassays.LC5e=2.014×104PIBs/eachlarva.The genomic DNA was digested with three restriction endonucleases BamHI,EcoRI and PstI,and 17,22 and ..., Abstract
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