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Issue of Virologica Sinica
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Review
Review
The effects of Chineee herbal metdicines(Ophiopogon japonieus,Astragalus membranaceus)and a traceelement(chromium)on the growth metabolism and the anti-Virus titer in cultured human and mousemyocardial cells were studied.The resul ts showed that these drugs and chromium could remarkably en-hance ..., Abstract
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Review
DS-polyacrylamide gel electrophoresis and Western blot analysis were carried out on the components of circulating immune complex(CIC) in sera of patients with epidemic hemorrhagic fever(EHF).After separtion in SDS-PAGE,the cumponents of CIC were stained with coomassie brilliantblue showing mainly 7 ..., Abstract
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Review
sensitive ELISA for detection of antibodies against hepatitis E virus(anti-HEV)was established,based on three synthetic peptides from open readin franmes ORF1,ORF2 and ORF3 of the HEVgenome.sera were tested for anti-HEV with our ELISA,One hundred and tWo(97.1%)out of 105sera from patients with ..., Abstract
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Review
The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain ..., Abstract
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Review
The BHK_21 cells infected with Beijing strain of fixed rabies virus were studied by electron mlcroscopy,The rod-like,bullet,round and oval viruses within the cytoplasm,mostly Iocated in the var-ious cisterna of rough and smooth endoplasmic reticulum and usually associated with homogeneous material ..., Abstract
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Review
probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity ..., Abstract
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Review
The HCV C_(33c) antigen and HBxAg were detected with monoclonal antibod ies against C_(33C) and Poly-clonal antibedies agalnst HBxAg by immunohistochemical techniquee in 86 paraffin-embeded sectionsof liver cirrhositic tissues,The rate of the HCV C_(33c) antigen and HBxAg positive was ..., Abstract
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Review
In antigen capture ELISA in which HCV peptides NS4 CP1 and CP2 derived from Hcv NS4and core gene region,respectively,were used as solid-phase antigens was developed to detect the differences in light chain isotype expresslion of anti-HCV antibodies. Antibodies in 65 sera of HCV infectedprofessional ..., Abstract
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Review
ymantria xylina Swinhoe nuclear polyhedrcais virus (LxNPV)is a kind of multi-embeded baculovirus. Scanning electron microscope(SEM)showed that the LxNPV inclusion bodies were irregularpolyhedra with different sizes.The average diameter of the polyhedra was 1.40μm.A lot of rod-shapedvirions which ..., Abstract
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Review
The present paper reported the effects of the ineeulum concentration of protecting strain and challenge strain,position of challange inoculation on the effectiveness of PRV mild strain cross-protection.Some technical aspocts in developing and implementing a cross protection program were ..., Abstract
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Review
ovine viral diarrhea virus(BVDV)InL genomic RNA was used as a template to synthesizeDNA by reverse transcriptase.The ds cDNA was synthesized by RNase H-DNA polymerase,and waslsetrted into the Pst I site of the pUC8 vector by dC-dG homopolymeric tailing and ligation.After beig transformed to E.coli ..., Abstract
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Review
The immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric ..., Abstract
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Review
poly(A)tRNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A)tRNA was identified through denatural agarose elec-trophoresis Six ..., Abstract
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Review
Poly(A) ̄+RNAs enriched with rabbit haemorrhagic disease virus(RHDV)proteins were isolatedfrom RHDV-infected rabbit liver tissue by polysome immunoprecipitation and purified by oligo(dT)-cellulose chromatography.The purified poly(A) ̄+RNA was identified through denatural agarose elec-trophoresis ..., Abstract
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Review
Rabbit haemorrhagic disease virus(RHDV)was purified from infected liver by freezing and thawing,chloroform extraction,PEG 6 000 precipitation and Sepharose-4B chromatography.Six polypeptides ,with the molecular weights of 64.5kD,61.4kD, 57.3kD,54.0kD.29.2kD and 28.1kD weredetermined by immuno ..., Abstract
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