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Issue of Virologica Sinica
Article List:
Review
3 strain of Japanese Encephalitis(JE)virus was adapted in Vero cells for 27 passages;Nosignlficant changes were found on virus titre and CPE after adaptation,but the immunogenicitywas getting weaker after 10 passages.The immunogenicity weakened could be recovered partlallyby repassag ing the V ..., Abstract
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Review
Rabbits were inoculated with EHFV-H114 strain,the distribution of virus antigen in thetissues of rabbits was studied by immunoflourescent technique and double bridge peroxidase(PAP)method.Meantinle,pathologic change in the tissues embedded in paraffin was observedby H-E staining.The results showed ..., Abstract
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Review
Lactosaminated Human Serum Albumin(L-HSA)was,used as a carrier and coupled to Ara-binosied adenine monophospate(AraAMP).The distribution of 125I-labelled L-HSA-AraAMPwere higher in liver than in other organs.Radioactivities of the drug could still be measured atthe 7th dav after injection.The ..., Abstract
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Review
Accordingto our computer analysis of hydrophobicity and prediction of secondary structuresfor the full-length putative proteins encoded by ORF 1(open reading frame-1),ORF2 and ORF3ofhepatitis E virus(HEV),we selected amino acid regions with tiydrophilicity,β-turn,and β-sheet,and synthesized seven ..., Abstract
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Review
This paper reports the protective effect of human rTNF-α and Human IFN-α on HEL andNEP-2 cell cultures infected by viruses in vitro,That is,before virus challenge,the treatment ofcell cultures in comparison with TNF-αand IFN-α may result in the inhibition of virus inducedcytopattiy.The ..., Abstract
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Review
Rabbits were inoculated with EHFV-H114 strain,the distribution of virus antigen in thetissues of rabbits was studied by immunoflourescent technique and double bridge peroxidase(PAP)method.Meantinle,pathologic change in the tissues embedded in paraffin was observedby H-E staining.The results showed ..., Abstract
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Review
This report confirms the sequence of pSTy 28 fragment of HHV-6.We jave determined thecomplete nucleotide sequence,The sequence data have revealed open reading frame(ORF)of 2414 nucleotides encoding a ribonucleotide reductase (RiR) protein of 805 amino acids and 1100nucleotides encoding a P41 ..., Abstract
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Review
Part of HCV core gene fragment is expressed in E.coli.The recombinant protein C27 is fu-sion protein with GST.The specific protein iS about 30%of total bacterial protein,After purifi-cation,the purity of the recombinant protein is more than 95%.We compared C27 antigen withC11 core antigen by EL ISA ..., Abstract
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Review
Two modified primers,△P3 and △P4,were designed and,for each of them,two mutatedbases were included.A fter PCR amplification, the DNA fragment contains a restriction endonu-clease recognition site,BamHⅠ or Hind Ⅲ at its ends,Cloning of the parvovirus H-1 NS-1 partialgene was conducted by double ..., Abstract
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Review
From 1983 to 1991,our collaborating research group of the project(one of the main stateproject of scientific and technical research during the period of The Sixth-Seventh Five Yearplan)collected and studied 3618 portions of san1ples of hot or sweet pepper virus disease in Bei-jing,Tianjin ..., Abstract
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Review
ne hundred and twenty nine samples of virus infected tobacco leaves were collected from 11counties/cities of Heilongjiang Province and Changchun City during early June to late August,1991-1993.Viruses were identified by differential hosts,serological assays(ELISA or DIBA)and electronmicroscopy of ..., Abstract
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Review
More than four PVY group viruses were found to infect Narcissus by the techniques of de-generate primer reverse transcription(RT)-polymerase chain reaction(PCR)and restriction frag-ment length polymorphism(RFLP),they may be TBV,NYSV,NSSV and OnYDV etc., Abstract
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Review
This paper reported that proviral DNA of bovine leukaemia virus(BLV)was detected bynested PCR.External and internal primers were selected from gp51 gene in BLV genome,Theproducts of the nested PCR were identified by 2%a8arose 8el electrophoresis and were com-flrmed by hybridlzation with a ..., Abstract
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Review
Small spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides ..., Abstract
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Review
Antiphage mutants selected by 15 phage strains have resistant only to its relevant phage,50it is narrow-spectrum antiphage strain.The fusant ZG88 is obtained with protoplast fusion be-tween Corynebacterium pekinense 7338 and Corynebacterium crenatum Ts-Li.It s serologic reac-tion and phage attach ..., Abstract
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Review
The virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The ..., Abstract
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