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Issue of Virologica Sinica
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Review
Review
Review
In this study,we detected anti-HEV and anti-HCV in a group of blood donors in Wuhan areabv enzyme linked immunosorbent assay.The anti-HEV and anti-HCV positive rates were 5.74%and 9.35%,respectively,In plasma donor group,the people who had a elevanted ALT historywere 14.04% for anti-HEV and 14.18% ..., Abstract
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Review
emorrhagic fever with renal syndrome(HFRS)virus RNA and antigens were simultane-ously detected by in situ hybridization and immunohistochemical techniques in the autopsy renaltissues of the patients with HFRS.In 36 cases collected separately from Shaanxi,Shenyang,Shanghai and Jiangxi HFRS endemic ..., Abstract
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Review
CV RNA and its NS3 region C33c antigen were detected by in situ hybridization withdigoxgenin-11-dUTP labeled HCV 5'non coding region cDNA probe and immunohistochemicalmethods with monoclonal antibody against recombinant HCV C33c protein in 35 cases of primaryintrahepatcr-cholangiocarcinoma(PIC) ..., Abstract
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Review
the recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.TheRVGG was promoted by pll promoter of V.V.The VVM11KRG does not contain lac gene.Thestudy showed that its titer is higher than ..., Abstract
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Review
he effect of Verapamil(Ver),Astragalus membranaceus(AM),Dexamethasone(Dex)on Ca ̄(2+) influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replica-tion in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found thatthe Ca ̄(2+) influx could be ..., Abstract
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Review
sing DPH fluorescence probe,the membrane of normal bone marrow cells infected withepidemic hemorrhagic fever virus(EHFV)was labeled.The membrane lipid fluidity could becounted according to change of value of fluorescence polarization.At the same time,using cyto-histochemical staining quantitative ..., Abstract
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Review
Genome of the characters for the viral multiplication and cytopathic changes in Bs484 cells afterinfection with Buzura suppressaria nuclear polyhedrosis viruses(BsNPV)were reported in thispaper,The results were as follow:1.The multiplication of progeny viruses showed differentchangeable forms in ..., Abstract
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Review
Ultrathin sections of leaf tissues of three tuber mustard varieties infected with Turnip MosaicVirus were examined in the electron microscope.The results revealed that the aggregates of fila-mentous virus particles,which always distributed near vacuole membrane and cytoplast in meso-phyll ..., Abstract
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Review
he cDNA sequences spanning nt 1209 to nt 1626 of Fny-CMV RNA2(a subgroupⅠstrain)and nt 2002 to nt 2433 of Ls-CMV RNA2(a subgroup Ⅱ strain)were used for probe preparation.The 32P-labeled negative strand RNAs prepared by transcription in vitro were hybridized with pu-rified RNAs of two Chinese CMV ..., Abstract
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Review
he influence of six antiphytoviral substances,including three base analogues and threemembrane lipid analogues,on the polymerization of coat protein of tobacco mosaic virus(TMV)was studied under different temperatures in vitro.The results demonstrated that all of the testedantiphytoviral substances ..., Abstract
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Review
The active Rabies virus suspension was cultured in microcarrier culture of five litres bioreac-tor.After virus suspension was havested from microcarrier culture,the prdcess comprised clarifi-cation by centrifugation and filtration,concentration by ultrafiltration,purification by gel filtra-tion.The ..., Abstract
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Review
wo viruliferous Rhopalosiphum padi(BYDV)can be detected by indirect enzyme-linkedimmunosorbent assaay(ELISA).Based on that result,a Aphid Acquisition-Enzyme-linked im-munosorbent assay(AA-ELISA)has been established for identification of barley resistance toBYDV.The OD value of AA-ELSA showed that ..., Abstract
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