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Issue of Virologica Sinica
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Review
Review
Review
Review
This study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal ..., Abstract
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Review
Mostly employing recombinant antigen,indirect enzyme linked immunosorbent assay is usedto detect HEV infection.Since synthetic peptides can be synthetised easily and have less nonspe-cific structures,they have been extensively used in the laboratory diagnosis of viral diseases.Basedon the reported ..., Abstract
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Review
eripheral blood samples from patients with exanthem subitum and lymphoproliferative dis-orders were collected.Mononuclear cells were separated from patients blood and were cultured invitro.As a result,virus strains were isolated from the peripheral blood mononuclaear cells of sevenpatients with ..., Abstract
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Review
The spleen cells isolated from SCID mice transplanted with human tonsillar lymphocytes(Hu- TLC-SCID)immunized with dengue virus 2 type(DV_2)in vitro and in vivo were trans-formed by Epstein-Barr virus(EBV).Specific human anti-DV_2 antibodies were detectable in thesupernatant of the transfomed ..., Abstract
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Review
he morphogenetic process of Acr NPV in vivo and cytopathology of its host cells were ob-served and analysed using electron micloscopy techniques after Acr NPV infected larval stagehost.The investigation shows:after innoculation 72~168h,profuse virogennic ..., Abstract
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Review
Two specific primers were designed and synthesized according to the genomic sequence ofPLRV reported in literature,The first strand of cDNA was synthesized by reverse-transcriptionusing RNA of PLRV Chinese isolate(PLRV-Ch)as a template,followed by PCR amplification.The synthesized cDNA was cloned ..., Abstract
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Review
The morphological and biological characters of three CMV isolates infecting banana fromGuangdong were studied.According to the different reactions in six kinds of host plants includingCucumis sativus,Nicotiana tabacum cv,HV38,Lycopersicon esculentum,Cucumis melover.saccharins,and sepecially Musa ..., Abstract
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Review
Rice stripe virus(RSV)genome is composed of four ssRNA species. The 5'-and 3'-terminal sequences of about 20 bases of each ssRNA were almost completely complementary to each otherand the 5'-terminal sequences of all four ssRNA have the some sequence of 11 nucleotides.Thoseconserved sequences were ..., Abstract
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Review
he whole sequence of RDV S1 fragment and partial sequence(S5Ⅲ)of RDV S5 fragmentwere synthesized and amplified by the method of reversed transcription-polymerase chain reaction(RT-PCR).Each amplified product was then cloned into pGEM7Zf(+)or pUC19 at the Smalsite,and sequenced.Based on these ..., Abstract
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Review
everal strains of barley yellow mosaic virus(BaYMV),each distinct from those recognisedin Japan and Europe,have been identified in China based upon the response of foriegn barley cul-tivars.These strains are identical in serological properties and can not be distinguished by ELISA and Western ..., Abstract
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Review
arek's Disease Virus(MDV)glycoprotein D(gD) gene was amplificated from CEF ge-nomic DNA infected with MDV GA strain by polymerase chain reaction(PCR).PCR products ofthe gD gene were labeled with digoxigenin as porbes and they could only detect MDV-CEF ge-nomic DNA,MDV genomic DNA Bam HI-A fragment ..., Abstract
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Review
Total RNAs were isolated from Heliothis armigera larvae infected with Heliothis armigeraNuclear Polyhedrosis Virus(HaNPV) late after infection(60 h postinfection),800 μg total RNAswere obtained from 1g larvae by improved granidinium isothiocgarate method.The whole viralDNA was purified from HaNPV ..., Abstract
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Review
The identification of a virus isolate of chinese chive which occurs in Fuxing city of Liaoningwas carried out during 1993~1995.The isolate can be transmitted by mechanical inoculation andaphid(Myzus persicae),but cannot be transmitted by seed and soil.The isolate only infected 8species of plant ..., Abstract
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