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Issue of Virologica Sinica
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Review
Review
Review
Review
high sensitive method of nested-polymerase chain reaction(Nested-PCR)was estab-lished to detect HBV-DNA in peripheral serum from 169 HBsAg or HBsAg/HBeAg positivepregnant women and their neonates。Positive of HBV-DNA was 72.8% in 103 positive of HB-sAg mothers and 33.0% in their neOnates.In the ..., Abstract
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Review
The present study reports the use of reverse transcriptase polymerase chain reaction(RT-PCR)for typing 18 independently isolated Hantaviruses from Asia-Pacific area by 2 sets of type-specific primers and the sequence analysis.The results of PCR showed that Hantaan specificprimers could amplify all ..., Abstract
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Review
Full-length cDNA coding HCV core protein was isolated from human HCV positiveserum by RT-PCR and inserted into baculovirus transfer vector.Sf9 insect cells were co-transfect-ed with the recombinant transfer vector plasmid and wild type baculovirus DNA.The recombi-nant baculoviruses containing gene ..., Abstract
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Review
epatitis C virus(HCV)5'non-coding region(5'NCR)cDNA with 302 bp obtained byreverse transcription polymerase chain reaction(RT-PCR)from the serum of a patient withchronic hepatitis C in Guangdong.Province was subsequently filled in recessed ends,purified andinserted into pUC19 plasmid vector.The ..., Abstract
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Review
here are high levels of TNF-α in the supernatants of lymphocyte culture infected with HHV-6 GS strain and the Nanjing loeal strains.TNF-α was detected by two methods:biological ac-tivity assay using L929 cells and ELISA.HHV-6 can induce TNF-α with in 24 h,maximal re-lease of TNF-α occurred ..., Abstract
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Review
ltrathin secction electron microscopy was used to investigate the morphology and morpho-genesis of rubella virus(RV)JR23 and Gos-10 strains in BHK21 cells and its effects on cellularultrastructures.The results showed that virions were observed in the cytoplasm at 6 h postinfec-tion and a large ..., Abstract
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Review
Antiviral effect of Alternanthera philoxeroides Griseb(APG)was studied in oral for epi-demic hemorrhagic fever virus(EHFV)infection in suckling mice.The APG dosages of A,BandC groups were 5mg/(g·d),7.5mg/(g·d)and 10mg/(g·d),respectively. The results showedthat the survival rate of A,B and C group ..., Abstract
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Review
virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only ..., Abstract
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Review
Pseudorabies virus(PRV)was mumplicated on BHK-21 monolayer cells.After concentra-tion with centrifuge,viral DNA was isolated by SD-proteinase K digestion method.According to the sequences of protein kinase genes of PRV Ka and NIA-3 strains,the primers with 26 bpand 32 bp were designed.With pured ..., Abstract
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Review
Using two kinds of spherical viruses from diseased Penaeus chinensis as antigens,the relativeantibodies were prepared.Then diseased shrimp samples from Jiangsu,Hebei,Qingdao,Fujianand Dalian were detected by counter immunoelectrophoresis,ELISA and immunological EM tech-nique.The results showed that ..., Abstract
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Review
new ELISA method was established for detecting specific IgG,IgM,IgA type circulatingimmune complexes(CIC)in sera of patients with Hemorrhagic Fever with Renal Sydrome(HFRS).The rabbit anti-HFRS virus antibody was used as coated antibody and anti-humanIgG,IgM,IgA monoclonal antibodies were used as ..., Abstract
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Review
RNA - dependent RNA polymerase extracted from the cucumber cotyledons four days afterinoculation by CMV-B-1 containing attenuation satellite RNA was used to initiate synthesis of(-)sense RNA from(+)sense RNA,using both CMV genomic or satellite RNAs as tem-plates.In presence of satellite RNA,the ..., Abstract
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Review
The results of computer analysis showed that,(1)47.8~66.7% seq homolo~ between CARNA5D (t-)RNA and PS『rVld(+)RNA;(2)48.1~63.4% sequeEtce homology be.tween CARNA5D(4-)RNA and PSTVd (一)RNA;(3)six dotplot between CARN^5D(+)RNA and PSTV_d(+)RNA;and(4)six base pairing regions between ..., Abstract
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Review
strain of new virus was isolated from fledgling budgetigars.The viruses were purified byusing Sepharose-2B chromatography.The size fo the virions was about 50 nm by electronic mi-croscopy.Pure virions were inoculated to healthy young fledgling budgerigars(not older than 5days).All of the birds were ..., Abstract
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