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Issue of Virologica Sinica
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Review
Review
随着对虾养殖业的发展,其病害亦日趋突出。对虾病原的研究,特别是病毒性病原的研究,是当前我国发展对虾养殖业的重要课题,它对虾病的防治与检测提供重要的科学依据。为此,我们对近十多年来已发表的有关对虾病毒方面研究的论文和资料作一概述,希望能对同行的研究工作..., Abstract
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Review
Review
The genes of poliovirus type I isolated from environmental water were studied by PCR (polymerase chain reaction). It was found that 22 strains of the virus were all Sabin-related. The result showed that the wild polioviruses in the environmental water were substituted by the sabinstrain completely ..., Abstract
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Review
The replication of dengue-2 virus in cultured human endothelial cells derived from humanumbilical veins was demonstrated. The viruses were detected in the supernatant as early as 12 h after infection by microplaque forming assay on C6/36 monolayer cells. The highest viral titers were obtained at ..., Abstract
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Review
Reverse transcription polymerase chain reaction was used to detect hepatitis C virus (HCV) RNA in 95 serum specimens of blood donors with anti-HCV IgG negative. Six out of 8 detections, HCV RNA was detected in 17 of 95 specimens (17. 9% ). These 17 HCV RNA positive specimens were detected once more ..., Abstract
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Review
The primary manufactory technique for Vero cell rabies vaccine was established. The quality of the experimental vaccine meet "the requirment for Vero cell rabies vaccine" introduced byWHO in 1986.The Vero cell strain used for the vaccine production was from ATCC(U. S. A. ). RFD virusstrain used as ..., Abstract
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Review
A partial gene of HGV was cloned and sequenced from the serum of a blood donor in Hebei Province of China. The partial sequence from the nostructural region gene showed 90. 5% nucleotide identity and 95 % amino acid identity over the corresponding region of isolates in America. One hundred and ..., Abstract
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Review
The cloned coat protein (CP) gene from Ys strain of papaya rinspot virus (PRV) was sequenced by dideoxy-chain termination method. Results show that the full length of the CP genewas 858 nucleotides (nt). Comparison among CP genes of 16 PRV strains or isolates from Chinaand abroad indicated that Ys ..., Abstract
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Review
Because of the close phylogenetic relationship, the simian acquired immunodeficiency syndrome (SAIDS) is considered as an important model in search of new drugs in prevention and treatment of the pandemic human AIDS. The simian AIDS type D retroviruses (SRV) and simian immunodeficiency viruses ..., Abstract
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Review
In vivo transfection with a plasmid-linked tandem DHBV DNA dimer on 2-day old DHBV-free Furong ducklings was carried out. Most of the animals (86%) developed transient viremia. Serum DHBs/pre S Ag and DHBV DNA appeared on 9th day, reached to its peak on12th~14th day and disappeared on 28th after ..., Abstract
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Review
The viruses were isolated by cocultivation of sheep foetal lung cells and the peripheral blood mononuclear cells of goats inoculated with ovine progressive pneumonia virus (OPPV). The result indicates that OPPV can infect goats. The isolated viruses were characterized by observations of ..., Abstract
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Review
By studying the flue structure of GCHV (Grass Carp Hemorrhage Virus), It was found that the capsid of purified GCHV can be degraded spontaneously into its morphological units by prologing in a buffer of low ionic strength, but not very completely. The virus preparation was obtained from GCHV ..., Abstract
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Review
This study showed that 300 μW/cm2 UV irradiation for 5 min could inactivate 94.63% ofHAV. No obvious changes were found experimentally under this irradiation dose, other than the decrease of RNA transfection ability. After 20 min UV irradiation, all of HAV were inactivated. For the RNA ..., Abstract
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Review
Peptides of coat proteins of cucumber mosaic virus isolates infecting banana from Guangdongprovince were first analysed. The coat proteins of different isolates were digested with Chymotrypsin and Pepsin to produce different peptide patterns. They were evaluated by Western blotusing the antiserum ..., Abstract
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Review
Serological characterization of three strains (BS, CS and MM) from Guangdong and one strain (HI) from Hawaii of cucumber mosaic virus infecting banana were first studied. These fourstrains were confirmed to belong to CMV DTL serotype. The serological relationships between BSand CS were closed while ..., Abstract
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