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Issue of Virologica Sinica
Article List:
Review
Review
Review
Review
In the present study, with a new foreign epitope - presenting system, FHV - RNA2 carrier system, on the precursor capsid protein of an insect virus, flock house virus (FHV) as a carrier, in recombinant baculovirus system and in pET system three epitopes derived from the amino acid residues ..., Abstract
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Review
Highly reiterated Sequence AGMr (Hind Ⅲ)-l was isolated from genome of Vero cell line derived from African green monkey and cloned into plasmid pUC18. Sequence analysis confirmed that the insert Sequence is right but 6 out of 172 bp had mutated in the 165th generation of Vero cell. Southern Blot ..., Abstract
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Review
In order to observe further dynamic changes on proliferation of HFRSV in Leptotrombidium Scutellare, the raised Leptotrombidium scutellare were ground and sterilized with an interval of 20-40 days, the filterate of each batch was inoculated separately into Vero - E6 cells, titres of HFRSV and ..., Abstract
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Review
Hantavirus (HV) has a tripartite, single - stranded, negative - sense RNA genome. Two pairs of oligonucleotides flanking S segment were chosen as primers for reverse transcription Polymerase chain reaction(RT - nested PCR). The method of RT - nested PCR was used for detection of virus RNA in HV ..., Abstract
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Review
The plasmid p CMVCVSRG containing the full- length cDNA of G protein of rabies virus CVS strain was constructed and transiently transfected into NIH 3T3 cell. The expression of Gprotein in transfected NIH 3T3 was confirmed by indirect immunfluorescent(IIF) and alkaline phosphatase anti-alkaline ..., Abstract
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Review
A hammerhead structure ribozyme was designed and synthesized to potato leafroll virus Chinese isolate (PLRV - Ch) genome within 356 to 358 nt "GUC" in coat protein (CP) gene. DNA sequence encoding the ribozyme was inserted into the position downstream from SP6 promoter of in vitro transcription ..., Abstract
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Review
A near full - length cDNA library of Wheat Rosette Stunt Virus (WRSV) was constructed.Purified viral RNA was polyadenylated by E. coli poly(A) polymerase. The poly(A) - tailed RNA was used as a template complementary to oligo dT(12 - 18) primer for cDNA synthesis.The cDNA was separately digested ..., Abstract
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Review
With electron microscope, a kind of spherovirus was observed in plasma of penaeid shrimp Puns Chinensis at different stages, such as eggs, larvae and juvenile shrimps. The artificial infection experiment verified that. the spheroviral particle has infectivity. The offsprings coming from carrying ..., Abstract
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Review
Three viruses were isolated from the kidney cell culture of Tupaia belangeri (tree shrew).They would like to replicate in mitosis of TL cells, to produce CPE and hemagglutinin and to hemaggulate the erythrocytes of guinea pig and mouse. The viruses belong to one serum type by hemagglutination ..., Abstract
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Review
With electron microscopy techniques the major virus pathogens of artificially infected Penaeus chinensis were studied. It showed that there are two kinds of virus pathogens in diseased shrimps: spherical virus and baculovirus. Sometimes they were co - existing in the same tissue or even in the same ..., Abstract
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Review
Titrating infective virus by plaque assay is one of the most accurate methods which could determind the numbers of infective virus. The result shows that a considerable number of vaccinia viruses in inoculum were still unadsorbed to cv - 1 cell monolayer after a period of 4 hours adsorption time. A ..., Abstract
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Review
Hierarchical clustering analysis have been done on 48 strains of the representative insects inclusion body viruses (26 strains of nuclear polyhedrosls viruses, 13 strains of granulosis viruses and 9 strains of cytoplasmic polyhedrosis viruses) by capillary gas chromatography, using the eight ..., Abstract
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Review
After infection with Trichoplusia ni nuclear polyhedrosis viruses, the esterases in the hemolymph of the fifth instar larvae of the Argyrogramma agnata were examined by the polyacrylamide gel electrophoresis (PAGE) and thin - layer scanning. The results showed that TnNPV can inhibite the metabolism ..., Abstract
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