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Issue of Virologica Sinica
Article List:
Review
Review
Review
Review
The pathogenicity of herpes simplex virus(HSV)in mice宙as observed.The mice appears
syndrome and sign afterinoculation 4 days HSV could beisolatedinthe blood on 2hours po$tin—
fection.The isolation rate of HSV and levd of viremia were increased on 48 hours postirfection.
Abstract
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Review
Tlle expression vector plasmid pBV220 was used tO expre~ ,the envelope曹lycuproteins,G1
andG2 ofFtantsall~vil-uJ5inE.co/i.First,the codingframegenesofG1 andG2werem plifiedby
PCR,and doned into the PCR product cloning T—vector,then they;were cut f u蜕|唱 抟s韵曲蛹n
Abstract
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Review
For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for  constructingGl and G1 expression plasmids, pGEX-4T-l  was a fusion-protein expression vector phsmid.The results showed that the ..., Abstract
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Review
NS 4 gene of hepatitis C virus was amplified by RT - PCR using RNA template isolated from anti - HCV positive serum of a blood donor from Hebei province in China. The amplified fragmint (abeut 900bp)was cloned into plasmid pET17Xb and the recombinant plasmid was des-ignated pETNS 4. SDS - PAGE ..., Abstract
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Review
The bifunction fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. The ..., Abstract
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Review
The preventive and curative effects of Astragalus membranaceus Bungo A6 on the mice in-fected with influenza virus FM1 strain were studied. The results showed that the gruffs concentra-tion of Astragalus membranaceus Bungo was the range of 1250~ 5000 mg/kg. d×5. The preven-tive and curative effects ..., Abstract
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Review
The polyhedrin gene of the Heliothis armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) was localized by Southern blot hybridization with a probe from the Autographa cali-fornica MNPV (AcMNPV) polyhedrin gene. Nucleotide sequencing showed the open reading frame of the HaSNPV polyhedrin gene ..., Abstract
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Review
One pair of oligonucleotide primers were designed to amplify the region of hog cholera virus (HCV)genome, which corresponds to a 588 bp portion encoding the major protective antigen re-gion, a part of gp55 protein. The product was amplified from two virus isolates (strains CC and JL)which had been ..., Abstract
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Review
The spherical virion with the size of 90~ 140 nm in diameter was detected in blood cell ma-trix of the feet, mantle and internal organization of diseased abalone. The virion was surrounded by dual unit membrane, no inclusion body. The morphology and size of virion in negative staining were the same ..., Abstract
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Review
With light and electron microscopy techniques, the pathological changes of seven tissues of Panaeid chinensis were studied. The major pathological changes mainly took place in the tissues of digestive system: hepatopancreas, midgut and stomach etc. Under light microscope, it was found that the ..., Abstract
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Review
Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of ..., Abstract
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