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Review
近几年来,基因治疗研究发展迅速,目的是将外源基因导入靶细胞,在体内产生疗效。早在1990年美国NIH的研究人员就将腺苷脱氢酶(adenosinedeaminase,ADA)基因导入病人的淋巴细胞,改善了由于ADA基因缺损造成的免疫缺陷., Abstract
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Review
Review
Review
The X gene of a known adr subtype of hepatitis B virus (HBV) was cloned into the ProEX HT Prokaryotic Expression System (GibcoBRL),and the X protein of HBV,154 amino acid residues,was expressed in a fusing fashion in E.coli . The fusion protein,named His tagged X protein (shortly HX protein) ..., Abstract
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Review
With the technique of immune electron microscope,the morphology and structure of HCV were studied.After homonization of liver tissue,immune precipation with McAb to HCV C region′s antigen, and negative staining,a kind of HCV associated virus like particles was visualized.They were mainly 55-56 nm ..., Abstract
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Review
Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The ..., Abstract
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Review
The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple ..., Abstract
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Review
Nucleotide sequence of the Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (HaSNPV) HindIII K fragment has been sequenced. The fragment comprises 3255 bp, containing 15 ORFs that are at least 40 amino acids in length, including 489 bp of the 3′end of polyhedrin(ph) coding ..., Abstract
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Review
The coat protein (CP) gene and 3′noncoding region of tomato mosaic virus Sl isolate (ToMV Sl) were amplified by RT PCR with specific primers designed on the basis of reported ToMV L strain (ToMV L) sequence. The cDNA fragment was cloned and sequenced,its sequence showed that the CP gene has 480 ..., Abstract
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Review
According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not ..., Abstract
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Review
The nucleotide sequence and location of the L4 nonstructural 100 K protein gene of the egg drop syndrome virus (EDSV),a strain AA 2 previously isolated from China,were determined.The 100K protein gene located at 55.7-64.8 m.u.has a length of  2091 nt and codes for a polypeptide of 696 amino ..., Abstract
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Review
Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence ..., Abstract
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Review
Two kinds of virus isolates P 14 and P a 1,P 33 were isolated from the mosaic,crinkled and stunted petunia (Petunia hybrida Vila) plants.The isolate P 14 was identified as cucumber mosaic virus (CMV) and the isolates P a 1 and P 33 as turnip mosaic virus (TuMV).CMV was the prevailing virus in ..., Abstract
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Review
A method for analysis of fatty acids in the insect inclusion body viruses by capillary gas chromatography was described. The main components of the fatty acid chromatogram were identified ,showing that the fatty acids in the granulosis virus and nuclear polyhedrosis virus were mainly composed of ..., Abstract
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Review
Five hybridoma cell lines secreting monoclonal antibodies(McAb) against hepatitis G virus NS 5 protein (HGV NS5) were raised by fusing Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant HGV NS5 protein. The results of indirect ELISA demonstrated that these McAbs have ..., Abstract
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Review
Sensitive and specific anti HCV diagnostic kits are very important for preventing of the transmission of hepatitis C virus. Adding HCV NS5A antigen in diagnostic kits may increase the sensitivity of diagnostic reagents. The result of sequencing of NS5A cDNA fragment showed that it shares over 90% ..., Abstract
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