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Issue of Virologica Sinica
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Review
Review
Review
To investigate the function of LTR in vaccinia virus, 6 recombinant viruses were constructed by inserting intact or serially truncated LTR together with their downstream of gag ORF of human immunodeficiency virus type 1 (HIV 1) into the vaccinia virus genome. Indirect fluorescence assay, Western ..., Abstract
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Review
Japanese Encephalitis (JE) Vaccine in Vero cell can be easily purified by zonal centrifugation at non continuous sucrose gradients (36% and 60%), 32 600g for 4h. The calf serum protein and other nonviral proteins in the vaccine were almost separated from the JE virus. The residual calf serum ..., Abstract
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Review
At three time points duing the period of five years, the hypervaviable region gene of HCV from two patients with HCV persistent infection was amplified, sequenced and analyzed. The results demonstrated that there were five significant characteristics in genetic variation of HCV HVR. 1. Wide ..., Abstract
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Review
GC32 cell line established by authors from a carcinoma patient is susceptible to JE and Chikungunya. The susceptibility of GC32 cell to JE and Chikungunya is similar to that of BHK21 regarded as control. BHK21 and GC32 cells infected by JE,Chikungunya and Langat viruses make “ +++~++++ ..., Abstract
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Review
The amino acid consensus sequences of membrane protein V3 region of HIV 1 Yunnan epidemic strain named YNV3 and two groups of amino acid consensus sequence YNV3A and YNV3B\ were deduced from DNA sequences of env gene V3 region of 16 local strains collected during 1992-1994,and analysed by DNASIS ..., Abstract
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Review
The local strain of human herpesvirus 6 (HHV 6),named CN5, was used to study some immunological properties of infected cells.By the methods of indirect immunofluorescence test, APAAP and MTT, the morphological and kinetic character of viral antigen expression, the changes of CD antigens expressions ..., Abstract
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Review
Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied. The minimal cell inoculation concentration was 1.0×10 5 cells/mL when Cytodex 3 microcarrier ..., Abstract
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Review
Using a transfer vector plasmid pSXIVVI +X3 without an initiation codon, the occluded recombinant Trichoplusia ni nuclear polyhedrosis virus as an expressing vector carrying the cDNA encoding gold fish growth hormone Ⅱ(gf GHⅡ) under the control of the SynXIV promoter has been constructed. ..., Abstract
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Review
p35 gene from baculovirus is an antiapoptosis gene which can sustain virus multiplication when infected by baculovirus. Both p35 deleted and inserting inactivated AcNPV can not replicate in Sf cell lines and Ha cell line.It is almost the same in BmNPV.Acp35Z which generated from AcNPV inserted LacZ ..., Abstract
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Review
Two flue cured tobacco cultivars, G28 and K326, from Guangdong,were transformed by CMV BS CP gene from diseased banana via Agrobacterium tumefaciens , and 313 transgenic tobacco plants were obtained. Calli were observed on the small square sections of leaves of the transgenic plants in culture ..., Abstract
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Review
Viral particles similar to non occluded baculoviruses were isolated from penaeid shrimp Penaeus chinensis in China. The size of enveloped virion is 250-300×110nm.After extraction,the viral DNA was digested by EcoRⅠ and cloned into the pUC 18. Two clones, L46 and M13,containing inserts of ..., Abstract
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Review
Differentiation of strong virulent and vaccinal Marek's disease virus (MDV) was carried out by using DNA molecular hybridization. DNA probe was made of BamHI L fragment from MDV gene library (strain GA) by labelled with digoxigenin using random priming. And the complete nucleotide sequence of BamHI ..., Abstract
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Review
A virus isolated from a diseased black locust tree (Robinia pseudo accacia) in the suburb of Beijing was identified as peanut stunt virus (PSV),designated PSV Robinia isolate (PSV-R). PSV-R infected 14 of 24 species of plants in four families. The thermal inactivation point was 55-60℃. The ..., Abstract
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Review
A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which ..., Abstract
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