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Issue of Virologica Sinica
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Review
Review
Review
The baculovirus insect cell expression system was used to prepare the HPV16 E 6 recombinant protein. The HPV16 E 6 complete ORF amplified by PCR from HPV16 genome was T A cloned into the baculovirus transfer vector pVL1393 T under AcMNPV Polh. This recombinant transfer plasmid pVL1393 E 6 DNA and ..., Abstract
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Review
The effect of Polygonum cuspidatum water extract (PCWE) on C57BL/6 mice infected with LP BM5 murine leukemia virus (MuLV) was investigated taking spleen index, viral antigen positive cell, serum IgG level and ConA response as parameters. It is demonstrated that PCWE can partly inhibit LP BM5 ..., Abstract
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Review
In order to find new anti human immunodeficiency virus (HIV) ribosome inactivating proteins (RIPs), the anti HIV activities of 47 RIPs and crude proteins from 17 species of plants were examined by the following measurements: 1) the inhibition of syncytia formation; 2) the protection of HIV 1 ..., Abstract
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Review
HPV 16 (HB strain) E 7 gene was cloned into expression vector pWR590 1 by gene cloning technique. Recombinant plasmid pWHB E 7 was obstained by analysis of restrictional endonuclease. 70 kD HPV 16 (HB strain) E 7 fusion protein was expressed by E.coli JM 109 transfected with pWHB E 7. The ..., Abstract
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Review
With in situ hybridization technique the localization and distribution of HSP70 mRNA and Hantaan virus RNA in the tissues of 30 cadavers with epidemic hemorrhagic fever (EHF), which were collected from different EHF epidemic foci in China, were examined. HSP70 mRNA was detected in most of the ..., Abstract
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Review
The gene encoding the N terminal 1-65 amino acids of preS1 was amplified by PCR and fused to the 3′ end of hTNF α gene to form a hTNF α preS1 fused gene which was directly inserted into the expression vector pSB 92 between EcoRⅠ and BamHⅠ sites and under the control of P 1 promoter. The fused ..., Abstract
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Review
In order to get high level expression of HIV 1 gp41 gene, a fragment (690 bp) encoding N terminal of gp41 from all sequence of HIV 1 was amplified by PCR. After digesting by restriction enzyme EcoRI/Sal I, the fragment was cloned into pET28a plasmid which was digested by the same restriction ..., Abstract
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Review
The multiple with other biological agent Autographa californica nucleopolyhedrovirus (AcMNPV) pesticide, which is widely used to control Spodoptera exigua and Prodenia litura as well as Artogeia rapat, Hellula untalis, Plutella xylostella , was selected. Tested with 2nd instar S. exigua larvae, the ..., Abstract
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Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity ..., Abstract
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Review
Through the reaction between GCHV 873 (Grass Carp Hemorrhage Virus) particles and random peptide library displayed on phage in vitro, after 3 rounds of affinity selection 16 phage clones from 300 clones have been got, which have high affinity with GCHV. Six of sixteen clones could inhibit the ..., Abstract
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Review
After infecting with Vaccinia virus, HeLa cells manifest typical morphological characters of apoptosis, and the electrophoretogram of their chromosomal DNA shows the ladder pattern. In situ apoptosis detection reveals that the location of the DNA cleavage is near the nuclear membrane, where ..., Abstract
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Review
A method of antibody capture polymerase chain reaction (AC PCR) is developed for the detection of human hepatitis A virus (HAV) in shellfish ( Scapharca subcrenata, Buditapes philippinarum, Macoma incongrua, Sinonovacula constricta, Chlamys farrer ). Initial virus extraction is based on a modified ..., Abstract
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