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Issue of Virologica Sinica
Article List:
Review
Review
Review
Ten strains of virus were isolated from vesicle fluid of skin lesions of 12 patients with variclla or herpes zoster by means of Vem-E6 eeUs.The postive rate was 83.3% These viruses all cause typical cell damages such as rounded and shrivelled,fusion and fallout.These damages got quick ..., Abstract
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Research Article
Six of anti HIV 1 sero positive plasma derived from a local high risk population were investigated by RT PCR and cDNA sequencing. The results indicated that HIV 1 prevalent in a local high risk population belonged to HIV 1 subtype B. The sequences of nucleic acids and amino acids in V3 loop of 6 ..., Abstract
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Research Article
The hemorrhagic Fever with Renal Syndrome Virus was exposed 0,30,60,90,120 minutes respectively on the surface of glass plate in the natural condition of indoor (dry bulb temperature: 27.5℃, wet bulb temperature: 25.2℃, RH 82%). The survival of them was observed although virus was exposed up to ..., Abstract
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Research Article
Detection had been made for the levels of serum specificity IgA, IgE antibodie and specificity of virus antigens circulation immune complexes (CIC) on the different days or phases of illness and clinic types in patients of HFRS. The results showed that the level of specific IgA antibody was higher ..., Abstract
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Research Article
A total of 19 367 adult female mosquitoes belonging to 16 species of Aedes genus were captured from nine countries, south western part of Yunnan Province, China, and were examined by C6/36 cell method and suckling mouse method. Two strains of Japanese encephalitis (JE) virus were isolated from 6491 ..., Abstract
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Review
HGV NS5 cDNA of two Hong Kong strains and one Guangdong strain were amplified by re—
verse transcription polymerase chain reaction (RT-PCR).T}le products were inserted into pUC19 vect0rs
respectively.~fter transfecting DH5a and JM 109 strains.the reeombinants were screened ..., Abstract
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Review
The mutated gene of dsFv to HBsAg by PCR based point mutagenesis method was constructed and sequenced. The mutated genes of VH and VL were cloned into plasmid pET 20 b and sequenced with the dideoxynucleotid method. The results showed that the cysteines were introduced into position 44 aa of VH and ..., Abstract
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Review
RNA of Hantaan virus (HNTV) strain Chen isolated from China was extracted from lysate of Vero E 6 cell infected by the virus. With the RNA as template, 1.3kb cDNA fragment containing the region encoding nucleocapsid protein (NP) was obtained by reverse transcription polymerase chain reaction (RT ..., Abstract
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Review
The HBV HBsAg coding region of a hepatitis B patient was analyzed by PCR amplification, bacteriophage cloning and nucleotide sequencing. It was showed that among different clones from the same patient there were point mutations and these different nucleotide changes were not resulted from the ..., Abstract
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Review
LsNPV DNA restriction fragments were hybridized with p 35 gene fragment of AcNPV. The 5.5 kb fragment of LsNPV was cleaved by single and double degestion and its physical map was constructed. After subcloning and sequencing, a intact ORF that is 906 bp in length, deduced amino acids are 302 in ..., Abstract
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Review
The intergenic sequence (IS) cDNA of Potato Leafroll Virus (PLRV) Chinese isolate was constructed into transformation binary vector pROK2 in positive or negative direction, then introduced into the commercial potato cultivar Desiree via Agrobacterium tumefaciens mediated gene transfer. Kanamycin ..., Abstract
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Review
Comparative studies on biological properties, physical and biochemical properties, morphology and serological relationships of five cucumber mosaic virus (CMV) isolates (PE,PE2,PEf,PC,PF) infecting Passiflora edulis, P. edulis × P.edulis var. flavicarpa, P.edulis var. flavicarpa, P.caerulea ..., Abstract
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Review
By two pairs of primers complementory to the published sequences at the both sides of EcoRI site and BamHI site on CAV circular genome, two DNA fragments containing two parts (1.5 kb and 0.8 kb) of CAV genome divided by EcoRI and BamHI sites were amplified by PCR from the circular CAV genome (2.3 ..., Abstract
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Review
Grass Carp Hemorrhage Virus (GCHV) was purified from infected fish cell culture by using different centrifugation. Its total genome was isolated by proteinase K treatment following phenol/chloroform extraction. The individual segment of GCHV dsRNA was separated by running 1% agarose gel ..., Abstract
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