Hello guest, Register  |  Sign In
Issue of Virologica Sinica
Article List:
Review
Review
Review
The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay,reverse transcription polymerase chain reaction and cy—
tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec—
Abstract
Free PDF (126.41KB)
Review
The S genome segment encoding the nucleocapsid protein (truncated) of Hantavirus H8205 was amplified by PCR and cloned into the expression vector pGEX 3X and expressed in E coli. SDS PAGE showed that the expressed fusion protein (GST NP) was mainly in the form of inclusion body. The expressed ..., Abstract
Free PDF (198.17KB)
Review
For investigating poliovirus recombinant defective vector system, the separated genes of poliovirus structure protein and replicase were induced into cell, the defective recombinant virus could be assembled. This virus is able to infect the cell and to express the extraneous gene in the cell, and ..., Abstract
Free PDF (215.31KB)
Review
Torch is the abbreviation of four pathgenic microbols: Tonoplasma gondic (Toxo), Rubella Virus (RuV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV 1 2 ). A new indirect ELISA reagent to detect the specific IgG and IgM antibody to ToRCH has been prepared. McAbs, labeled with horseradish ..., Abstract
Free PDF (184.91KB)
Review
Three rhesus monkeys ( Macaca mulatta ) were inoculated intravenously with fecal suspensions obtained from hepatitis E patients. The alanine aminotransferase (ALT) level in the three monkeys elevated 3 to 4 weeks after infection. Virus like particles about 27 to 34 nm were found in the stool ..., Abstract
Free PDF (228.32KB)
Review
Hepatitis G virus E2 glycoprotein gene was amplified by polymerase chain reaction, and was inserted into baculovirus expression vector pF AST B AC HTa, constructing a recombinant transposing vector pF AST B AC E2. The plasmid pF AST B AC E2 was transformed into DH10B AC competent E. coli cells. ..., Abstract
Free PDF (157.87KB)
Review
The apoptosis induced by influenza virus in cultured MDCK cells was reported and the selective inhibitory effect of melamin on the apoptosis induced by influenza virus was investigated. The results showed that the DNA ladder could be first detected at 6 h post infection (p.i.), accompanied by ..., Abstract
Free PDF (182.23KB)
Review
The sequences of HGV gene that had been reported were checked and analyzed. After comparison of HGV seqences from different virus strains, a gene fragment of 31 nucleotides (7121-7152 nt) served as a probe was labeled by fluorescein N 6 dd ATP with terminal transferase. The specificity of HGV probe ..., Abstract
Free PDF (166.78KB)
Review
An infectious genome of the Periplanta fuliginosa densovirus (PfDNV) has been cloned into the bacterial plasmid pUC119. The viral genome could be rescued from the recombinant plasmid PfNDV pUC119 by transfection to sensitive Periplanta fuliginosa larvae. Virions can be found in the cells of the ..., Abstract
Free PDF (149.15KB)
Review
Review
A couple of CSFV specific primer(PF 5648 and PR 6604)was designed with the aid
of computer primer designation software and synthesized based upon the relative conserved regions
of published sequenL-~s of C strain.NS2-3 gene(p125 gene)fragment of CSFV Shimen strain was
Abstract
Free PDF (162.99KB)
Review
The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM ..., Abstract
Free PDF (143.81KB)
Review
The cellular tota1 RNA was isolated from 1ivers of the died giant panda.then the tern
plates were produced by reverse transcription reaction.Two couple primers were applied to ampli—
fy the 3 end of fusion protein gene of canine distemper virus(CDV)giant panda strain About
Abstract
Free PDF (172.83KB)
Review
The coding region of S genorae segment of Hantaan virus(76/118 strain)was inserted
into the eukarytic expression plasmld pVR1012 The recombinant expression plasmid pVRS22
was c。nstructed.Ver0一E cells were transiently tmnsfected in vitro with pVRS22 plasmid.The
transient ..., Abstract
Free PDF (90.43KB)
Recent Issues:
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved