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Issue of Virologica Sinica
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Review
Review
Review
Seven cases of chronic hepatitis B virus carriers were included for sequencing of whole gene sequences of hepatitis B virus(HBV).The serotype of 4 strains of HBV weYe adr,and 3 strains were adw.Two strains were dassifled to genotype B.an d the other 5 strains to genotype C.No significant ..., Abstract
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Review
A cell line persistently infected by Hepatitis A virus (HAV) was ultilized to produce high yield viral particles of HAV. HAV particles were recovered in a single ultracentrifugation step through discontinuous sucrose/glycerol density gradients. Three kinds of major structural protein of HAV were ..., Abstract
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Review
With polymerase chain reaction (PCR) a fragment encoding the 28 kDa protein of wheat yellow mosaic virus (WYMV) RNA2 was amplified and constructed into plasmid of pET11a for prokaryotic expression. BL21 (DE3) of \%E.coli\% transformed with the recombinant plasmid of pE2839 was induced to ..., Abstract
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Review
According to the differential pH precipitation of proteins, two methods were established for purification of disease specific protein (SP) in rice plants infected by rice stripe virus (RSV), a member of \%Tenuivirus.\% The yields obtained by the two methods were 0.8 and 2.0 mg SP per g of infected ..., Abstract
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Review
By conventional EM technique and DGD embedment free section EM technique together with the procedure of gentle extraction, the assembly and release of simian AIDS type D retrovirus (SRV) in infected Raji cells were studied. The results showed that SRV nucleocapsids were assemblied in some cytoplasm ..., Abstract
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Review
The j5R open reading frame (ORF) of African swine fever virus was predicted by computing to encode a 12.9 kDa protein with two successive N terminal transmembrane domains and one C terminal antigenic epitope. Antibodies raised against a synthetic peptide derived from the C terminal epitope detected ..., Abstract
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Review
The primers for RT PCR were designed on the basis of M and N gene sequence of PRRSV. A gene fragment about 900 bp, which has digestion sites of EcoRI and BamHI, was amplified by RT PCR. The M and N gene were cloned into expression vector pBV220 and one recombinant PBVMN was constructed which highly ..., Abstract
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Review
Bovine spumavirus (BSV) 3026 China strain, which was isolated by authors, encodes the transcriptional transactivator Borf 1. The Borf 1 protein, as well as the BSV3026, can transactivate the gene expression directed by bovine immunodeficiency virus long terminal repeat (BIV LTR) in transient ..., Abstract
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Review
The recombinant transfer plasmids pSXIVVI\++X3 S1.Holte and pSXIVVI\++X3/4 S1.Holte containing S1 gene cDNA of avian infectious bronchitis virus and the parent virus (TnNPV SVI -G) DNA were used to co transfect \%Spodotera frugiperda\% (Sf) cells. The recombinant viruses TnNPV (X3)S1.Holte OCC + ..., Abstract
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Review
A 1.8 kb fragment of Newcastle disease virus (NDV) haemagglutinin neuraminidase (HN) gene was amplified by polymerase chain reaction (PCR) with primers containing enzyme cleavage sites, and inserted into a nonessential gene of herpesvirus of turkey (HVT). Recombinant viruses were screened by Dot ..., Abstract
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Review
A strain of new virus was isolated from fledgling budgerigars by authors in 1995 Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.The SDS-PAGE proved that viral capsidxgas composed of eight ..., Abstract
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Review
The fermentation broth of yeast strain SH\-2?(FSH\-2) which could enhance the biological potency of human interferon alpha (huIFN α) was detected. Its enhancing ratio was 1.64~6.86 fold. A group of proteins was seperated and purified from the fermentation supernatant by chromatography on ..., Abstract
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Review
The arrangement patterns of nucleocapsids within the envelope of \%Autographa californica\% nuclear polyhedrosis virus were studied by electron microscopy. Numbers of nucleocapsids observed in an envelope in their cross sections ranged from 1 to 17, and the frequencies at 2,3,4,5,6 and 7 ..., Abstract
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