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Issue of Virologica Sinica
Article List:
Review
Abstract:Two promoters.LTR (1ong terminal repeat)and IP (internal promoter),exist in
genomes of foamy viruses.Ge1l transfection and transient expression assay demonstrated that!the
basal activity of BFV (bovine foamy virus)IP is much higher than that of BFV LTR;transacti.
Abstract
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Review
In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results ..., Abstract
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Review
In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results ..., Abstract
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Review
HCMVs in blood leukocytes and in serum from the same blood donor were examined by polymerase chain reaction (PCR) and DNA hybridization technique. In the mean time, HCMV IgM and HCMV IgG in the serum were examined by ELISA. Two hundred blood leukocyte samples and 200 serum samples were examined in ..., Abstract
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Review
The HIV 1 p24 gene (gag gene) was amplified by polymerase chain reaction (PCR) and cloned into an E.coli expression vehicle pQE30 between BamH I and Kpn I sites. After induction with IPTG, the transformed E.coli strain M15 expressed the HIV 1 p24 gene efficiently. The recombinant p24 protein was ..., Abstract
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Review
The target fragment of HGV genes including C, E1, E2, NS2 and partial NS3 regions was microinjected into the pronuclei of mouse fertilized eggs according to the standard methods. Ten mice containing the HGV genes were identified by the screening of polymerase chain reaction with the primers in NS3 ..., Abstract
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Review
In order to understand the characteristics and rules of HIV diversity when passaged in vitro , the systematic study on the variation of env gene of HIV strain introduced from American in 1988 was carried out. Taking all the following strains as the objects: the 96 strain derived from the primary ..., Abstract
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Review
From the sera of 3 Chinese patients with hepatitis C, 3 new nucleotide sequences of the 5′ non coding region (5′NCR) of hepatitis C virus (HCV) were found using reverse transcription polymerase chain reaction (RT PCR) and DNA recombinant and sequencing techniques. Comparing with ..., Abstract
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Review
Insect Sf 9 cells were infected with constructed recombinant virus AcMNPV OCC - GST 6xHis Etp 28, which could produce GST fusion protein and the supernatant of 72 h pi cell lysate was examined by SDS PAGE. The results showed that GST 6xHis Etp 28 fusion protein of 53 kDa was expressed in insoluble ..., Abstract
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Review
The development of Dendrolimus punctatus wenshanensis Cytoplasm Polyhedrosis Virus (DpwCPV) insecticide is reported. The formulation is an oil in water emulsion and the concentration of polyhedrosis is 2.5×10 9 PIB/mL. The auxiliary materials come from the agriculture products. It is safety ..., Abstract
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Review
The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. ..., Abstract
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Review
The Antiviral substance was induced from blastulae embryonic cell line of crucian carp (CAB) by ultra violet inactivated grass carp reovirus (GCRVuv). This substance was sensitive to trypsin and stable at 56 ℃ pH 2—11. The activity of this substance was influenced by target cell density ..., Abstract
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Review
Some multiplication properties of the Lapinized Chinese strain of Classical Swine Fever Virus (CSFV C strain) in primary bovine testicular cells were studied. One CSFV C strain which can be detected by immunofluorescence technique was found and a new method in titering called flurescent plaque ..., Abstract
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Review
The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases ..., Abstract
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Review
The morphogenesis of the Grass Carp Reovirus (GCRV) was studied in CIK cells. The CIK cells were infected by the GCRV at MOI (multiplicity of infection) of 5 10 PFU/Cell. It was demonstrated that the subviral particles without outer layer proteins could be seen in the cytoplasm at 4 hours post ..., Abstract
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Review
The RNA of dengue 2 virus (New Guinea C strain, NGC) was extracted from small amount of dengue 2 virus culture supernatant. The large NS3 gene fragment was amplified from RNA of dengue 2 virus with RT PCR method. The amplified NS3 gene fragment was cloned directly into the pUC18 plasmid vector. The ..., Abstract
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Review
A 1.66 kb expected S1 gene fragment of avian infectious brochitis virus (IBV) H strain was amplified by RT PCR. Its PCR/RFLP pattern was similar to IBV D 41 strain and M 41 strain when digested using BstYI, HaeⅢ and EcoRI respectively. IBV H strain was thought as Massachusetts serotype., Abstract
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Review
Based on the known RNA sequence of rice grassy stunt virus , IRRI isolate (RGSV IR), the cDNA of nucleocapsid protein (NCP) gene was obtained by RT PCR, with genomic RNAs of Shaxian isolate (RGSV SX) as template. The cDNA was then cloned into pGEM T vector and sequenced. The results showed that the ..., Abstract
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Review
The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core+NS3) and pHCV NC (NS3+Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was ..., Abstract
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