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Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively. Inhibited proliferation of SUNE 1 was only observed in the ..., Abstract
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Recently, a novel virus named TT Virus has been identified in serum from patients with elevated ALT levels and others. TTV was detected by PCR using a set of primers from conserved region in the TTV genome. cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and ..., Abstract
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The expressing vector carring various truncated fragments of S gene of HTNV strain 76 118 was constructed and the vector efficienly expressed in E.coli . The result demonstrated that the GST NP fusion proteins exist in the form of inclusion bodies in E.coli , the expressing amount accounted for 29 ..., Abstract
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Q Sepharose F.F. was adopted to purify the suspension of oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Using column chromatography on Q Sepharose F.F. to purify the ..., Abstract
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Partial genomes of three strains of sporadic hepatitis E virus (Ch T11,Ch T21,Ch T40) were cloned and sequenced. The nucleotide and putative amino acid homology of Ch T11,Ch T21,Ch T40 and other HEV strains were 78.3 81.3% and 95.8 97.9%, respectively. The nucleotide homology between Ch T21 and Ch ..., Abstract
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In order to understand the mechanisms of hepatitis B virus (HBV) in the development of hepatocellular carcinoma, the nested PCR was used to amplify HBV core promoter from tumour tissues of 14 HBV DNA positive patients with hepatocellular carcinoma, and 10 serum samples of hepatitis B patients and ..., Abstract
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The serum from patients infected with Hepatitis E was prepared, the RNA of Hepatitis E virus was purified. The interested cDNA fragment (1.3?kb) was obtained by RT PCR, cloned in to the plasmid pUC18 and sequenced by the dideoxynucleotide method (fluorescein labeled dNTP). The result of sequencing ..., Abstract
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The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat ..., Abstract
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According to the genomic sequence of PLRV S, two pairs of specific primers were designed and synthesized. The cDNA of the replicase gene of PLRV Chinese isolate (PLRV Ch) was synthesized and amplified by RT PCR using the viral RNA as a template. The synthesized 3′ and 5′ cDNA fragments ..., Abstract
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Four kinds of eukaryotic expressing plasmids of classical swine fever virus (CSFV) E2 gene, either containing or lacking the signal sequence and transmembrane region sequence, were constructed. The antibodies against CSFV were induced after immunization of mice by intramuscular (IM) injection with ..., Abstract
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Primers for RT PCR were designed on the basis of the M and N gene sequences of U. S. isolate of PRRSV. The primers have restriction endonuclease ( Eco R I and Bam H I) sites. The tissues of stillborn fetuses of affected swine were detected by RT PCR in 4 pig farms. The samples were RT PCR positive. ..., Abstract
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A high virulent isolate of White Spot Disease Virus has been got in Fuzhou. The virion is in cytoplasm. Intact virion is enveloped and bullet shaped, d=80-100 nm. There is no inclusion body in cell, but sometime a particular structure named occlusion body (some virions are occluded by membrane) in ..., Abstract
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The cDNA sequence encoding porcine growth hormone (pGH) was inserted into the plasmid of pBluescript M13( ). The sequening results showed that a 984 base pair fragment is identical to that reported by Seeburg et al ., with the exception of one different base pair in the ORF. Then, a baculovirus ..., Abstract
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The sequence analysis of the haemagglutinin (H) protein gene of giant panda strain of canine distemper virus (CDV) from giant panda in China was conducted and compared with Onderstepoort vaccine strain. Four pairs of primers were designed and synthesized. The PCR products amplified from GP strain ..., Abstract
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An icosahedral virus was found in the ultra thin section of spleen, kidney, liver, gill of the outbreak infectious Siniperca chuatsi by electron microscope. The virions of section show hexagon, about 125 150 nm in diameter, and have envelope outside. Cell culture indicated that CIK cell was ..., Abstract
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Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One Eco R I fragment of 707 bp was selected and contains a putative open reading ..., Abstract
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Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template. The amplified fragment (about 1.8 kb) was cloned into plasmid pQE30 and the recombinant plasmid was expressed in JM109. The NS3 protein was purified by NiSO 4 ..., Abstract
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