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Issue of Virologica Sinica
Article List:
Review
The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two ..., Abstract
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Review
The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two ..., Abstract
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Review
The growth characteristics of Human herpesvirus 7 local strain YY5 were investigated in cord blood mononuclear cells (CBMCs) and CD4 + lymphoblastic cell line SUPT1. Virus growth was demonstrated by cytopathic effect (CPE), percentage of dead cells count, indirect immunofluorescence test with viral ..., Abstract
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Review
Preparation of infectious RNA by in vitro transcription from full length cDNA has led to advances in the study of many positive strand RNA viruses. There are two strategies of preparing full length cDNA of RNA viruses: (1)construction of infectious clone. (2)amplification of full length cDNA by ..., Abstract
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Review
To culture human bone marrow stromal cells long term in vitro , and investigate their susceptibility to virus, the fetus, child and adult bone marrow stromal cell were cultured for long time in vitro by the adherent assay. The myoid bone marrow stromal cells were obtained after being generated more ..., Abstract
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Review
Objective\ To study the relationship between the changes of Interleukin and Receptor in serum of patients with chronic hepatitis B (CHB) before and after Interferon α (IFN α) treatment and expression of HBV marker. Methods: Enzyme liked immunosorbent assay (ELISA) were performed in 25 ..., Abstract
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Review
A general cDNA library which was from infected larvae dueing the latent periods has been constructed. The first cDNA strand was synthesized from total RNA by M MLV transcriptase. After oligo(dG) tailing the cDNA was amplified by polymerase chain reaction. The double strands cDNA were ligated to ..., Abstract
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Review
The structure of nuclear matrix in Tn5B1 cell infected with AcMNPV was observed by means of sequential cell fractionation and whole mount technique of electron microscopy. Whereas early infection of AcMNPV resulted in no dramatic change in the nuclear matrix morphologically, polyhedra formed in the ..., Abstract
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Review
A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The ..., Abstract
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Review
Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). The detection sensitivity of indirect ELISA using antiserum against fusion protein GST NC was 1 mg of infected leaves or 84 ng of purified virus. The method of dot ..., Abstract
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Review
Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 5×10 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and ..., Abstract
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Review
A virus strain like calicivirus was isolated from organs of tiger with feline kidney (FK) cell. The viruses were nonenveloped, 35~39 nm in diameter, multiplied in cytoplasm and put in crystal order. The viruses multipied easily in feline kidney cell cultures and produced cytopathic effects (CPE). ..., Abstract
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Review
The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. ..., Abstract
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Review
The genomic DNA library of goose derived adenovirus (strain Y 81 G 4) was constructed after ten fragments of viral genomic DNA digested with Hin dⅢ were cloned into plasmid pUC18 respectively. The inserted fragments of the ten recombinant plasmids were labelled with digoxigenin. Viral genomic DNA ..., Abstract
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Review
In order to find out the reason for causing shiver disease of Chinese mitten crab ( Eriocheir sinensis ) a virus was isolated from diseased Chinese mitten crab. Symptom of normal crab was positive after it had been infected artificially with preliminary pure virion from the shiver crab. It was ..., Abstract
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Review
Complementary DNA of partial HCV NS2 NS3 protein gene which encodes viral Zn 2+ dependent metalloprotease and serine protease was amplified by RT nested PCR from serum of a Chinese patient with chronic hepatitis C.The product was digested with Eco R Ⅰ/ Xba Ⅰ and cloned into pcDNA3.The nucleotide ..., Abstract
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Review
Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obtained by PCR with primers specific to whitefly transmittal gemniviruses,cloned and ..., Abstract
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Review
The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of ..., Abstract
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