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Issue of Virologica Sinica
Article List:
Review
Mx proteins are members of a family of interferon inducible genes expressed in organisms or cells which are treated with all sorts of inducers or virus infection.These proteins together with other interferon inducible proteins form the antiviral state in host cells and the first line of the body’s ..., Abstract
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Review
We outlinethe development ofthe hantaanvims vaccine in ree~t years∞ brain dsaneinavtivated
vaccine,cultural cell bivalent and univalent inactivated vaccine,recu~ inadou vaccine,DNA vaccine,attenuat—
ed live vaccine,adjuvarIt,inacfivatima methods and lmnlulle strategy., Abstract
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Research Article
The aim ofthis stud),istoinvestigatethemechanis~s of apoptosisin polymorphonudear neutrophils
(PMNs)induced by simian immunodeficiency v~ (siv).In this experiment.gag gene was amplified by the
polymerase chaill reactio~andthe expression ofp53 and bc/一2 gC~12e were determined byWestern ..., Abstract
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Research Article
Abstract:Our pmvious studv showedthatK24 strain懈arI antigenicity broadHantavims SE0 type strain.In
this paper we studied the molecular characteristics of K24 strain.The total ceUular RNA Wilts extracted from in.
fected cell culture andtheM and S segment genewere ampficated byRT-PCR ..., Abstract
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Research Article
For further studying on the molecular characteristics of A16 strain,which is the seed virus of HFRS candidate vaccine,the M and S segments of A16 strain were amplified by RT PCR and then sequenced respectively.Sequence assay showed that Msegment of A16 strain contained 3615 nucleotides,encoding ..., Abstract
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Research Article
In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase ..., Abstract
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Research Article
HCV RNA and HBV X gene were detected by in situ hybridization and HCV core?C33c antigens and HBxAg were localized by immunohistochemical method.Results showed(1) The positive rate of HCV RNA and HBV X gene were 40%(55/136) and 82% (112/136) in HCC respectively,both of them were positive ..., Abstract
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Research Article
ODV E66 is the major protein of occlusion derived virions envelope of baculovirus.An odv e66 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified and sequenced.The HaSNPV odv e66 gene had a size of 2 109 bp and potentially coding a protein of 672 amino acids,with a ..., Abstract
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Research Article
An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30~-33nt ..., Abstract
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Research Article
In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8~59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular ..., Abstract
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Research Article
CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After ..., Abstract
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Research Article
In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a ..., Abstract
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Research Article
A strain of Spodoptera exigua Nuclear Polyhedrosis Virus(SeNPV)isolated from China was purified and identified.The results of bioassay indicated that the LC 50 of 2nd and 3rd instar of Spodoptera exigua was 6.6 ×10 4 and 2.6×10 5 PIB/ml, Abstract
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Research Article
The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression product(P 102 ) on Helicoverpa armigera nuclear polyhedrosis virus(HaNPV)and Bt against the 2nd instar larvae of ..., Abstract
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Research Article
The coat protein(CP)gene of an isolate of papaya malformed leaf virus(PMaLV),preserved in Plant Virology Laboratory of South China Agricultural University by Luo Xuehai,was synthesized by reverse transcription and polymerase chain reaction(RT PCR) and cloned into pGEM T and pGEM T Easy Vector ..., Abstract
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Research Article
A polydnavirus(PDV)was purified by sucrose density gradient centrifugation from the calyx region of female parasitoid wasp Microplitis mediator (Hymenoptera:Braconidae).Negative stain technique showed that the virus was tadpole like and measured 130×35nm.A diverse protein profile of the ..., Abstract
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Research Article
A pair of primers were designed and synthesized according to the reported S1 spike protein gene sequence of IBV.The viral RNA of SD/97/02 strain was amplified by reverse transcription polymerase chain reaction(RT PCR).The amplified product was analyzed by agarose gel electrophoresis,all appeared a ..., Abstract
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Research Article
The structure and morphogensis of Mandarin fish( Siniperca chuats ) virus were studied.The matured virons were about 135nm±10 in diameter with envelope outside.The intact virus is composed of three parts,its nucleocapsid was about 90nm±5,and the envelope was around 18nm±3,the air space between core ..., Abstract
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Research Article
To screen the antigenic epitope with monoclonal antibody (mAb)5H5 against Hantaan virus nucleocapsid protein from phage-displayed constrained nonapeptide library,we have completed the work as follows.(1) Purification and biotinylation of mAb 5H5.(2)Biopanning of the phage peptide library by using ..., Abstract
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Brief Reports
The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was ..., Abstract
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Brief Reports
Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health ..., Abstract
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