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Issue of Virologica Sinica
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Contents
In our study on the gene response to virus infection,a colony of 705bp—HSP一714 was se—
lected from the cell specific cDNA library induced by HSVI infection.The gene sequence analysis re·
vealed that it was a new complete gene sequence(Gene Bank Accession number~AF372624)with a ..., Abstract
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Contents
In order to evaluate the cytotoxicity and antiherpesvlral activity of penciclovir,the antiher—
pesvlral effect of PCV was investigated by observing eytopathie efect(CPE)and the change of viral
infectious titre jn cells infected with herpesvirus,antiviral index and treatment index,The ..., Abstract
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Research Article
In order to analyze HEV genotypes isolated from sporadic acute hepatitis E in Shanghai,
HEV RNAs were detected with RT—nPCR and the positive PCR products were cloned and sequenced
The genotypes of the isolated sequences were analyzed with the molecular biological software.The re— ..., Abstract
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Research Article
The one step growth curve of YN87448 strain virus in BHK21 cell showed that a lot of
progeny viruses could be observed at 16 hr.after infection The tiler of virus reached the highest about
10一 0TCDS0 at 48 hour post infection.The titer of virus is not reduced by Co1.The apo ptosis was ..., Abstract
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Research Article
A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV
(pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas—
setts for neomycin—resistance and EGFP.Vector stocks weir,prodaced by cationic lipid reagents co — ..., Abstract
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Research Article
In order to investigate the genomic organzation of the S/,o&’ptera litura multinucleoeapsid
nucleopolyhedrovirus(SphNPV),the EcoR 1-G[ragment located at map units(mu)49 2-54.1 ol the
viral genome was sequenced.The fragment contains 2 partial and 5 complete open reading frames ..., Abstract
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Research Article
The Xba I-I fragment located at 18.4~22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which ..., Abstract
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Research Article
Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes ..., Abstract
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Research Article
We have studied amphificatlon of DpCPV W in vitro in Sf21 cell ln this paper.The results
showed that DpCPV W could amplify is vitro in Sf2I ce t[and form normal potyhedra Acce~ional TeV
eombinant enhancln in medium coutd synergist the infective rate of DpCPV—W in Sf21 cel1 in vitro. ..., Abstract
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Research Article
An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng,Jiangsu,China
was used tO inoculated maize tO propagate the virus Improved single primer amplification technique
was adopted tO clone the vira1 genomic dsRNA SIO for sequence determination The result showed:the ..., Abstract
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Research Article
According to the Genbank open sequence,two pairs of primer were designed in order to am —
plify gE gene and partial gI gene of pseudorabies virus Shanghai strain,respectively.The g£gene and
partial gI gene were obtained by po]ymerase chain reaction(PCR),subsequently cloned into pGEM T ..., Abstract
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Research Article
Abstract:Two i.5kb DNA were amplified by reverse transcription and PCR(RT—PCR)from the ep—
ithelial tongue tissue of cattle infected with FMDV akesu/58 strain after 53 passages,and then cloned
A continuous 2,724 nuclco tlde sequence spanning the P3 codiiag region was ..., Abstract
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Research Article
Foot and mouth disease virus strain China/99 RNA5 were used as templet for RT—PCR.The
amplifled eDNA products were cloned into pGEM—T Easy Vectors and transformed into JM109.The re—
combinant plasroids were identified by electrophoresis.E∞R l cleavage,and PCR The nucleotide and ..., Abstract
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Research Article
Three HN genes of NDV isolates from South China were am plified by one-step RT-PCR .
They where subsequently cloned and sequenced. The Phylogenesis of these NDV isolates was also
studied.The gene types of So uth China NDV strains were found to be separated from those of Eum — ..., Abstract
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Research Article
The glycoprotein G (gG)gene of pseudorahies virus Hubei strain(PRV HB)was amplified
by PCR,sequenced and analyzed.The results revealed that the fragment cloned and sequenced was
1804 base pairs(bp)with a G+C content of 68.78% and an ORF containing 1500bpto encode a pro—
rein of 500 ..., Abstract
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Research Article
Abstract:The entire S1 genes from nephropathognic strain JS/95/03 and respiratory strain SD/97/01
of infectious bronchitis virus were amplified,cloned and sequenced The nucleotide and deduced amino
acid sequences of S1 genes from two isolates and ten reference strains were compared. ..., Abstract
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Research Article
Some characterizations of four Grass Carp Reovirus (GCRV) strains were comparatively studied at the first time. It showed that the stored GCRV\-\{873\},GCRV\-\{875\} and GCRV\-\{876\} at minus 30℃ for ten years still remained infectious to CIK cells, and the 3 virus titers could reach above 10\+2 ..., Abstract
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Research Article
Abstract:Some characterizations of four Grass Carp Reovirus(GCRV)strains were comparatively
studied at the first time.It showed that the stored GCRV873,GCRVs75 and GCRv876 at minus 3012 for
ten years still remained infectious to CIK cells,and the 3 virus titers could reach above 10 ..., Abstract
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Research Article
Abstract:The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho—
mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by
gel filtration chromatogmph with Sepia,arose 4B.The eluates collected were concentrated at 4℃ ..., Abstract
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Brief Reports
Abstract:PP7I gene Of the Hut~tan cytomegalor virur(HCMV AD一169)strain W,qS amplified by PCR.
After digestion by Barn H I and Hin dⅢ.the fragment was cloned into the high level expre~ion vec—
tot pET28a Recombinant plasmid pET28a—PP71 was transfonned into E.coli BI 2l(DE3)and ln— ..., Abstract
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Brief Reports
The research Of the cooperative antivirus activities was carried OUt using anaantadine. rib—
avirin and herhy houttuynia.The inhibitory effect 0f CPE caused by influenza virus A3 in MDCK cd
and the therapeutic effect on BALB/c mice pneumonia caused by influenza virus FM1 were examined ..., Abstract
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