Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan.The products contained within G 1 gene of M segm ent, w ere digested with H in C II and Sac I respectively, thereby, ..., Abstract
Abstract:The E gene of dengue一2 virus was amplified using RT—PCR mothod from the C6/36 cells in— fected by D2V NGC strain and inserted into pPICZ a B vector.The recom binant plasm id was integret— ed into Pichia pastoris X一33 by electroporation and the expressed products were ..., Abstract
Using HCV NS3 m onoclonal antibody as selective molecule, a 12 m ar phage peptide library was biopanned and positive clones were selected by ELISA . com petition assay and DN A sequencing. Eleven positive clones were chosen for DN A sequencing.From the experim ent and sequencing com ..., Abstract
To sequence the full length genome of Japanese encephalitis virus attenuated strain SA14—12— 1—7.direct information about the genomic structure and the possible relationship to the attenuated and stability m echanism has been provided in this study.Six pairs of prim ers were designed ..., Abstract
In order tO study the structure specificity of HPV 1 6 E 7 gene of cervical carcinoma in W uhan city and W ufeng county of Hubei the tissue DNA was abstracted from cervical carcinom a biopsies from W uhan and high incidence region of cervical carcinoma in W uhan county. HPV 16 E 7 gene was ..., Abstract
For understanding the mechanism Of neurovirulence attenuation Of the Japanese encephalitis virus(JEV),nucleotides of the E coding region of five JE strains,one wild and four attenuated viruses, were am plified by PCR and sequenced.A comparison of the five sequences indicated that there ..., Abstract
In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i,the cDNA of L segment was amplified fragment by fragment by RT—PCR .The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced.The I genom e segm ent is 6533 ..., Abstract
H antaan virus glycoprotein G1 and nucleoprotein partial fragment w ere connected in differ— ent ways.The constructed chim eric gene G 1 SO .7 or SO.7G 1 was inserted into baculovirus expression vector pFBD.The recombinant shuttle plasmids(Bacmids)containing chimeric genes were ..., Abstract
Abstract:To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden— ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD),the interaction of Ad E1 B 55kD with hDaxx was analyzed by the yeast two-hybrid assay, and the direct binding of Ad E1 B 55kD with ..., Abstract
An lef一3 gene of HaSNPV was identified.It was 1ocalized in Bam H 工一F fragment of the genome.Nucleotide sequencing showed the open reading frame was 1 1 40 nt,encoded 379 amino acids with a predicted size of 44kD.A ”TATA”box was found in 40~ 43nt upstream of the transcriptiona1 ..., Abstract
WHs3 Serratia marcesscens is a high yield bacterium of Chitinase.The yield of chtinase in induce medium was 84.4t~g/mI .The chitinase(A3) can enhance the toxicity of Helicoverpa arm igera nucleopolyhedrovirus(HaSNPV)up tO 20% ~70% .The LT50 and LT90 were shortened 1.1 and 1.3 ..., Abstract
Transgenic chili pepper plant expressing CM V and TM V coat protein gene were infected with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus content in inoculated plants.The results showed that the transgenic chili pepper exhibited high level ..., Abstract
Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de— signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR.The gene was cloned into pGEX一2T vector and then transformed into E .coli DH5a.The gene has high homolo— gy(95% ..., Abstract
An infectious bursal disease virus(IBDV)field strain,named NH99,was isolated and iden— tifed from some flocks with imm une failure in Shanghai according tO the clinical symptom ,pathology, serological test,animal challenge and virus morphology,The virus was characterized as a very ..., Abstract
From the tiger frog virus(TFV)genome,an open reading frame(ORF),whose deduced amino acid sequence(aa)shows identity with RNase 111。was identified.The ORF has 1 113bp long and codes a putative protein of 371 aa with a predicted molecular mass of 40.47kD and a G+ C content of ..., Abstract
Two different reoviruses were isolated during investigation on pathogens of diseased crab E— riocheir sinensis(designated as Es RV816 and Es RV905). RV816 was isolated from crabs collected from Jiangsu province.Es RV905 was purchased from a local farm in W uhan, Hubei province.The ..., Abstract
Acording tO Avian Infectious Brochitis virus(IBV)Beaudette strain S 1 gene sequence,a pair of primers were designed and synthesized.W ith the primers, IBV Guangdong isolation strain GD05 S 1 gene was successively amplified by RT — PCR .PCR product was digested w ith BstY I,Hae III ..., Abstract