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Issue of Virologica Sinica
Article List:
Research Article
Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of
34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan．The products contained
within G 1 gene of M segm ent， w ere digested with H in C II and Sac I respectively， thereby， ..., Abstract
34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan．The products contained
within G 1 gene of M segm ent， w ere digested with H in C II and Sac I respectively， thereby， ..., Abstract
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2002 Vol. 17 (3): 195-197
Research Article
Abstract：The E gene of dengue一2 virus was amplified using RT—PCR mothod from the C6／36 cells in—
fected by D2V NGC strain and inserted into pPICZ a B vector．The recom binant plasm id was integret—
ed into Pichia pastoris X一33 by electroporation and the expressed products were ..., Abstract
fected by D2V NGC strain and inserted into pPICZ a B vector．The recom binant plasm id was integret—
ed into Pichia pastoris X一33 by electroporation and the expressed products were ..., Abstract
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2002 Vol. 17 (3): 198-201
Research Article
Using HCV NS3 m onoclonal antibody as selective molecule， a 12 m ar phage peptide library
was biopanned and positive clones were selected by ELISA ． com petition assay and DN A sequencing．
Eleven positive clones were chosen for DN A sequencing．From the experim ent and sequencing com ..., Abstract
was biopanned and positive clones were selected by ELISA ． com petition assay and DN A sequencing．
Eleven positive clones were chosen for DN A sequencing．From the experim ent and sequencing com ..., Abstract
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2002 Vol. 17 (3): 202-205
Research Article
To sequence the full length genome of Japanese encephalitis virus attenuated strain SA14—12—
1—7．direct information about the genomic structure and the possible relationship to the attenuated and
stability m echanism has been provided in this study．Six pairs of prim ers were designed ..., Abstract
1—7．direct information about the genomic structure and the possible relationship to the attenuated and
stability m echanism has been provided in this study．Six pairs of prim ers were designed ..., Abstract
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2002 Vol. 17 (3): 206-210
Research Article
In order tO study the structure specificity of HPV 1 6 E 7 gene of cervical carcinoma in W uhan
city and W ufeng county of Hubei the tissue DNA was abstracted from cervical carcinom a biopsies from
W uhan and high incidence region of cervical carcinoma in W uhan county． HPV 16 E 7 gene was ..., Abstract
city and W ufeng county of Hubei the tissue DNA was abstracted from cervical carcinom a biopsies from
W uhan and high incidence region of cervical carcinoma in W uhan county． HPV 16 E 7 gene was ..., Abstract
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2002 Vol. 17 (3): 211-215
Research Article
For understanding the mechanism Of neurovirulence attenuation Of the Japanese encephalitis
virus(JEV)，nucleotides of the E coding region of five JE strains，one wild and four attenuated viruses，
were am plified by PCR and sequenced．A comparison of the five sequences indicated that there ..., Abstract
virus(JEV)，nucleotides of the E coding region of five JE strains，one wild and four attenuated viruses，
were am plified by PCR and sequenced．A comparison of the five sequences indicated that there ..., Abstract
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2002 Vol. 17 (3): 216-220
Research Article
In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i，the
cDNA of L segment was amplified fragment by fragment by RT—PCR ．The purified PCR products were
sequenced directly or cloned into pM D18一T vector and then sequenced．The I genom e segm ent is 6533 ..., Abstract
cDNA of L segment was amplified fragment by fragment by RT—PCR ．The purified PCR products were
sequenced directly or cloned into pM D18一T vector and then sequenced．The I genom e segm ent is 6533 ..., Abstract
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2002 Vol. 17 (3): 221-225
Research Article
H antaan virus glycoprotein G1 and nucleoprotein partial fragment w ere connected in differ—
ent ways．The constructed chim eric gene G 1 SO ．7 or SO．7G 1 was inserted into baculovirus expression
vector pFBD．The recombinant shuttle plasmids(Bacmids)containing chimeric genes were ..., Abstract
ent ways．The constructed chim eric gene G 1 SO ．7 or SO．7G 1 was inserted into baculovirus expression
vector pFBD．The recombinant shuttle plasmids(Bacmids)containing chimeric genes were ..., Abstract
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2002 Vol. 17 (3): 226-229
Research Article
Abstract：To question the reason of CO—localization of hDaxx and PM L in PODs disrupted by Aden—
ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD)，the interaction of Ad E1 B 55kD with
hDaxx was analyzed by the yeast two-hybrid assay， and the direct binding of Ad E1 B 55kD with ..., Abstract
ovirus(Ad)E1 B 55一kilodalton oncoproteins(Ad E1 B 55kD)，the interaction of Ad E1 B 55kD with
hDaxx was analyzed by the yeast two-hybrid assay， and the direct binding of Ad E1 B 55kD with ..., Abstract
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2002 Vol. 17 (3): 230-233
Research Article
An lef一3 gene of HaSNPV was identified．It was 1ocalized in Bam H 工一F fragment of the
genome．Nucleotide sequencing showed the open reading frame was 1 1 40 nt，encoded 379 amino acids
with a predicted size of 44kD．A ”TATA”box was found in 40～ 43nt upstream of the transcriptiona1 ..., Abstract
genome．Nucleotide sequencing showed the open reading frame was 1 1 40 nt，encoded 379 amino acids
with a predicted size of 44kD．A ”TATA”box was found in 40～ 43nt upstream of the transcriptiona1 ..., Abstract
Research Article
WHs3 Serratia marcesscens is a high yield bacterium of Chitinase．The yield of chtinase in
induce medium was 84．4t~g／mI ．The chitinase(A3) can enhance the toxicity of Helicoverpa
arm igera nucleopolyhedrovirus(HaSNPV)up tO 20％ ～70％ ．The LT50 and LT90 were shortened
1．1 and 1．3 ..., Abstract
induce medium was 84．4t~g／mI ．The chitinase(A3) can enhance the toxicity of Helicoverpa
arm igera nucleopolyhedrovirus(HaSNPV)up tO 20％ ～70％ ．The LT50 and LT90 were shortened
1．1 and 1．3 ..., Abstract
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2002 Vol. 17 (3): 239-242
Research Article
Transgenic chili pepper plant expressing CM V and TM V coat protein gene were infected
with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus
content in inoculated plants．The results showed that the transgenic chili pepper exhibited high level ..., Abstract
with CM V and TM V to com paring the characteristic of CP—M R in transgenic chili pepper and virus
content in inoculated plants．The results showed that the transgenic chili pepper exhibited high level ..., Abstract
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2002 Vol. 17 (3): 243-247
Research Article
Based on the PVS coat protein gene sequence(885bp)a pair of specific primers were de—
signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR．The gene
was cloned into pGEX一2T vector and then transformed into E ．coli DH5a．The gene has high homolo—
gy(95％ ..., Abstract
signed and the coat protein(CP)gene of potato virus S(PVS)was amplified by RT—PCR．The gene
was cloned into pGEX一2T vector and then transformed into E ．coli DH5a．The gene has high homolo—
gy(95％ ..., Abstract
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2002 Vol. 17 (3): 248-251
Research Article
An infectious bursal disease virus(IBDV)field strain，named NH99，was isolated and iden—
tifed from some flocks with imm une failure in Shanghai according tO the clinical symptom ，pathology，
serological test，animal challenge and virus morphology，The virus was characterized as a very ..., Abstract
tifed from some flocks with imm une failure in Shanghai according tO the clinical symptom ，pathology，
serological test，animal challenge and virus morphology，The virus was characterized as a very ..., Abstract
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2002 Vol. 17 (3): 252-256
Research Article
From the tiger frog virus(TFV)genome，an open reading frame(ORF)，whose deduced
amino acid sequence(aa)shows identity with RNase 111。was identified．The ORF has 1 113bp long
and codes a putative protein of 371 aa with a predicted molecular mass of 40．47kD and a G+ C content
of ..., Abstract
amino acid sequence(aa)shows identity with RNase 111。was identified．The ORF has 1 113bp long
and codes a putative protein of 371 aa with a predicted molecular mass of 40．47kD and a G+ C content
of ..., Abstract
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2002 Vol. 17 (3): 257-262
Research Article
Two different reoviruses were isolated during investigation on pathogens of diseased crab E—
riocheir sinensis(designated as Es RV816 and Es RV905)． RV816 was isolated from crabs collected
from Jiangsu province．Es RV905 was purchased from a local farm in W uhan， Hubei province．The ..., Abstract
riocheir sinensis(designated as Es RV816 and Es RV905)． RV816 was isolated from crabs collected
from Jiangsu province．Es RV905 was purchased from a local farm in W uhan， Hubei province．The ..., Abstract
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2002 Vol. 17 (3): 263-265
Brief Reports
Acording tO Avian Infectious Brochitis virus(IBV)Beaudette strain S 1 gene sequence，a
pair of primers were designed and synthesized．W ith the primers， IBV Guangdong isolation strain
GD05 S 1 gene was successively amplified by RT — PCR ．PCR product was digested w ith BstY I，Hae
III ..., Abstract
pair of primers were designed and synthesized．W ith the primers， IBV Guangdong isolation strain
GD05 S 1 gene was successively amplified by RT — PCR ．PCR product was digested w ith BstY I，Hae
III ..., Abstract
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2002 Vol. 17 (3): 266-269
Review
昆虫杆状病毒(Insect baculo irus)对鳞翅目、双
翅目和膜翅目等昆虫具有病原性，是一种开发应用
较广、高效的生物杀虫剂，具有杀虫专一、效果好、有
流行传播作用等优点。为了更好地利用昆虫杆状病
毒为防治农林害虫服务，加快昆虫杆状病毒杀虫剂
研究的步伐，本文归纳了近几年来有关昆虫杆状病 ..., Abstract
翅目和膜翅目等昆虫具有病原性，是一种开发应用
较广、高效的生物杀虫剂，具有杀虫专一、效果好、有
流行传播作用等优点。为了更好地利用昆虫杆状病
毒为防治农林害虫服务，加快昆虫杆状病毒杀虫剂
研究的步伐，本文归纳了近几年来有关昆虫杆状病 ..., Abstract
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2002 Vol. 17 (3): 270-275
Review
树匡鞫(Tupaia)是一种在生物医学研究中很有应
用价值的新型实验动物，其分类尚有争议，有人认为
是食虫类，有人则将之列为低等灵长类，还有人认为
树鳓是一个独立目的，称为攀鼢目(Scandentia)⋯ ，
但目前基本定为低等灵长类。由于其具有体积小， ..., Abstract
用价值的新型实验动物，其分类尚有争议，有人认为
是食虫类，有人则将之列为低等灵长类，还有人认为
树鳓是一个独立目的，称为攀鼢目(Scandentia)⋯ ，
但目前基本定为低等灵长类。由于其具有体积小， ..., Abstract
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2002 Vol. 17 (3): 276-278
Review
水生甲壳类动物病毒的研究始于一种地中海螃
蟹(Portunus depurator或Liocarcinus depurator)体
内发现的类呼肠孤病毒⋯ 。随后，人们相继在这类
动物体内发现多种病毒，其中约17种为杆形病毒。
这些杆形病毒的多数往往只是被它们的宿主携带
(尤其是螃蟹和淡水甲壳类动物)而不引发疾病，其 ..., Abstract
蟹(Portunus depurator或Liocarcinus depurator)体
内发现的类呼肠孤病毒⋯ 。随后，人们相继在这类
动物体内发现多种病毒，其中约17种为杆形病毒。
这些杆形病毒的多数往往只是被它们的宿主携带
(尤其是螃蟹和淡水甲壳类动物)而不引发疾病，其 ..., Abstract
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2002 Vol. 17 (3): 279-285
Review
肠道病毒感染是人类急性和慢性心肌炎的常见
病因之一，而且也与人类扩张型心肌病(DCM)的发
生发展有密切关系⋯ 。病毒分离，血清学研究，免
疫组化技术、原位核酸杂交技术以及聚合酶链反应
技术(PCR)均提示肠道病毒与心肌炎关系密切。最
近，LI等[ ]用肠道病毒特异性单克隆抗体，采用改 ..., Abstract
病因之一，而且也与人类扩张型心肌病(DCM)的发
生发展有密切关系⋯ 。病毒分离，血清学研究，免
疫组化技术、原位核酸杂交技术以及聚合酶链反应
技术(PCR)均提示肠道病毒与心肌炎关系密切。最
近，LI等[ ]用肠道病毒特异性单克隆抗体，采用改 ..., Abstract
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2002 Vol. 17 (3): 286-288
Review
自Kerr等1972年提出细胞凋亡(apoptosis)概
念以来，这一生物学现象的研究已受到广泛重视，细
胞凋亡是细胞在一定的生理或病理条件下，遵循自
身程序进行自杀的过程。但它与程序化细胞死亡
(PCD)不是同一现象⋯1。程序化细胞死亡具有严格
的基因时控性，一定时间内使某些细胞死亡而让另 ..., Abstract
念以来，这一生物学现象的研究已受到广泛重视，细
胞凋亡是细胞在一定的生理或病理条件下，遵循自
身程序进行自杀的过程。但它与程序化细胞死亡
(PCD)不是同一现象⋯1。程序化细胞死亡具有严格
的基因时控性，一定时间内使某些细胞死亡而让另 ..., Abstract
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2002 Vol. 17 (3): 289-295
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