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Issue of Virologica Sinica
Article List:
Brief Reports
A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing ..., Abstract
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2003 Vol. 18 (5): 411-414
Research Article
Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density
gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS）and
inf~ted by EB virus(EBV)，in Order to establish immortalized B lymphoblastoid cell line(B—LCL)．
B—LCLs were ..., Abstract
gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS）and
inf~ted by EB virus(EBV)，in Order to establish immortalized B lymphoblastoid cell line(B—LCL)．
B—LCLs were ..., Abstract
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2003 Vol. 18 (5): 415-419
Research Article
Abstract：In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1
(HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by
restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector ..., Abstract
(HIV-I)core protein gene(pCI—neoGAG)，gag gene was acquired from the plasmid pKSGAG by
restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector ..., Abstract
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2003 Vol. 18 (5): 420-422
Research Article
Abstract：T0 investigate the replication and expression of Hepatitis C virus(HCV)1 a and lb chimeric
cDNA in HepG2 cells．HCV l a／ l b chimeric CDNA was used to construct an expression plasmid for the
tran sfection of HepG2 cells．HCV protein and HCV genomic RNA an d an ti—genomic RNA in ..., Abstract
cDNA in HepG2 cells．HCV l a／ l b chimeric CDNA was used to construct an expression plasmid for the
tran sfection of HepG2 cells．HCV protein and HCV genomic RNA an d an ti—genomic RNA in ..., Abstract
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2003 Vol. 18 (5): 423-427
Research Article
Abstract：To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial
gene(502—1326bp)an d obtain data for serotyping antigen，the prokaryotic expression vectors pRSET
A—S，pRSET A—S—C were constructed respectively．The recombinant expression plasmids were ..., Abstract
gene(502—1326bp)an d obtain data for serotyping antigen，the prokaryotic expression vectors pRSET
A—S，pRSET A—S—C were constructed respectively．The recombinant expression plasmids were ..., Abstract
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2003 Vol. 18 (5): 428-431
Research Article
Abstract：The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and
cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR)．Positive
vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee，the nucleotide ..., Abstract
cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR)．Positive
vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee，the nucleotide ..., Abstract
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2003 Vol. 18 (5): 432-436
Research Article
To amplify M fragment from unknown Phleboviruses，members of 7 serocomplexes and 8 no
complex assigned Phleboviruses，total 42，were chosen and tested．Tl1e M segment amino acid sequences
Of 4 Phleboviruses in GenBank were aligned．Conserved regions were selected to design primers． ..., Abstract
complex assigned Phleboviruses，total 42，were chosen and tested．Tl1e M segment amino acid sequences
Of 4 Phleboviruses in GenBank were aligned．Conserved regions were selected to design primers． ..., Abstract
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2003 Vol. 18 (5): 437-440
Research Article
Abstract：To develop live—vectored vaccine of Human immunodeficiency virus (HIV-I)without
selectable marker,we first constructed a tran sfer plasmid pVI75 with selectable m arkers of neo an d／acZ
gene，an d a recombinant plasmid pVI75一Gagpol containing the gagpol gene of Chinese ..., Abstract
selectable marker,we first constructed a tran sfer plasmid pVI75 with selectable m arkers of neo an d／acZ
gene，an d a recombinant plasmid pVI75一Gagpol containing the gagpol gene of Chinese ..., Abstract
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2003 Vol. 18 (5): 441-445
Research Article
Abstract：ChemokiRe receptor CCR5 and CXCR4 are principal coreceptors for HIV Blockage of
Human immunodeficiecy virus(HIV)infection can be achieved by engaging CCR5 and CXCR4 with their
natural ligands． man herpesvirus 8 encodes three chemokines： vM IP1．vMIP2 and vM IP3．vM Ⅱ’2
has ..., Abstract
Human immunodeficiecy virus(HIV)infection can be achieved by engaging CCR5 and CXCR4 with their
natural ligands． man herpesvirus 8 encodes three chemokines： vM IP1．vMIP2 and vM IP3．vM Ⅱ’2
has ..., Abstract
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2003 Vol. 18 (5): 446-450
Research Article
Abstract：Nucleocapsid gene of SARS·-associated coronavirus(SARS·-CoV)was obtained by reverse
tran scription and polymerase chain reaction from a pateint sufered from severe acute respiratory
syndrome(SARS)from Beijing，subsequently cloned into pUCm—T vector．The sequence of positive ..., Abstract
tran scription and polymerase chain reaction from a pateint sufered from severe acute respiratory
syndrome(SARS)from Beijing，subsequently cloned into pUCm—T vector．The sequence of positive ..., Abstract
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2003 Vol. 18 (5): 451-453
Research Article
Abstract：A strain of Avian leukosis virus(ALV．J1 subgroup J was isolated from an adult broiler
breeder folck．which had a history of myeloid leukosis(ML)．in Inner Mongolian．Moreover,another
strain of ALV J was isolated from a ML case in Shandong．The two isolates were positive reaction ..., Abstract
breeder folck．which had a history of myeloid leukosis(ML)．in Inner Mongolian．Moreover,another
strain of ALV J was isolated from a ML case in Shandong．The two isolates were positive reaction ..., Abstract
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2003 Vol. 18 (5): 454-458
Research Article
Abstract：A baby hamster kidney cell line(BHK一21) infected by foot—and-mouth disease virus
(OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32
positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— ..., Abstract
(OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32
positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— ..., Abstract
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2003 Vol. 18 (5): 459-463
Research Article
Aquareoviruses，which belong to a newly identified genus of the family Reoviridae，are
agents infecting all kind of aquatic animals．Grass carp reovirus(GCRV)was isolated from outbreak
grass carp fingerling in South China freshwater region，and Threadfin reovirus(1f1W )is a Singapore ..., Abstract
agents infecting all kind of aquatic animals．Grass carp reovirus(GCRV)was isolated from outbreak
grass carp fingerling in South China freshwater region，and Threadfin reovirus(1f1W )is a Singapore ..., Abstract
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2003 Vol. 18 (5): 464-467
Research Article
The US2 gene nonessential for viral replication was amplified by polymerase chain
reaction(PCR)with DNA from CEF cells infected by MDV vaccine strain CV1988 as template，and was
cloned into T-easy vector；Th e expression cassette including GFP gene controlled by CM V promoter
and ..., Abstract
reaction(PCR)with DNA from CEF cells infected by MDV vaccine strain CV1988 as template，and was
cloned into T-easy vector；Th e expression cassette including GFP gene controlled by CM V promoter
and ..., Abstract
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2003 Vol. 18 (5): 468-472
Research Article
In order to develop new vaccine candidate of Equine infectious anema virus(EIAV)，env gene
of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the
downstream of pE／L promoter of pSC65 vector,separately．The resulted recombinant vaccinia ..., Abstract
of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the
downstream of pE／L promoter of pSC65 vector,separately．The resulted recombinant vaccinia ..., Abstract
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2003 Vol. 18 (5): 473-477
Research Article
Abstract：Oedaleus asiaticus entomopoxvirus(DaEPV) contains a factor which enhances the
infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis．When the spheroids of
OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged，the enhancing
activity ..., Abstract
infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis．When the spheroids of
OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged，the enhancing
activity ..., Abstract
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2003 Vol. 18 (5): 478-481
Research Article
Abstract：The coding region of superoxide dismutase was amplified by using PCR method from
Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus(HaSNPV)C 1 genome．The
PCR product was cloned into pGEM —T-easy vector and sequenced．Th e cloned coding region of
HaSNPV ..., Abstract
Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus(HaSNPV)C 1 genome．The
PCR product was cloned into pGEM —T-easy vector and sequenced．Th e cloned coding region of
HaSNPV ..., Abstract
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2003 Vol. 18 (5): 482-485
Research Article
Abstract：Periplaneta fuliginosa densovirus(pfDNV)was found in China at the first time，has been
classified into the subfamily Densovirinae in Parvoviridae．However,Which genus that it be longs to has
not yet been determined and is the focus of the present work．Recent advances in the ..., Abstract
classified into the subfamily Densovirinae in Parvoviridae．However,Which genus that it be longs to has
not yet been determined and is the focus of the present work．Recent advances in the ..., Abstract
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2003 Vol. 18 (5): 486-491
Research Article
Abstract：Ectropis obliqua nucleopolyhedrovirus(EoNPV)infects tea looper Ectropis obIiqua speci—
tically．The application techniques of EoNPV preparations were studied in this paper．It was suggested
that the optimum applied periods were 1～ 2 instar larvae of the 1st，the 2nd，the 5th and ..., Abstract
tically．The application techniques of EoNPV preparations were studied in this paper．It was suggested
that the optimum applied periods were 1～ 2 instar larvae of the 1st，the 2nd，the 5th and ..., Abstract
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2003 Vol. 18 (5): 492-495
Research Article
Abstract： gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT．PCR and then
cloned into pGEM—Teasy．Sequence analysis showed that its nucleotide sequence had high identity to a
Japan ese isolate of RDV an d contmned high percent of rare codons． l6 gene was expressed m ..., Abstract
cloned into pGEM—Teasy．Sequence analysis showed that its nucleotide sequence had high identity to a
Japan ese isolate of RDV an d contmned high percent of rare codons． l6 gene was expressed m ..., Abstract
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2003 Vol. 18 (5): 496-499
Research Article
To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23
strain，to express E2 protein in BHl(2 l cells，and to analyze the antigenic sites of E2 protein of RV JR23
strain．E2 gene of RV JR23 strain was amplified bv I PCR．The PCR product was cloned into the ..., Abstract
strain，to express E2 protein in BHl(2 l cells，and to analyze the antigenic sites of E2 protein of RV JR23
strain．E2 gene of RV JR23 strain was amplified bv I PCR．The PCR product was cloned into the ..., Abstract
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2003 Vol. 18 (5): 500-502
Brief Reports
Abstract：The nostructural protein(NS 1)encoded by gene 8 of the Influenza virus is present in cells
infected with Influenza virus．In this study,NS 1 protein gene of the Chicken influenza virus
A／chicken／Beijing／2／97(H9N2)strain was amplified by PCR．The fragment contains EcoR I and ..., Abstract
infected with Influenza virus．In this study,NS 1 protein gene of the Chicken influenza virus
A／chicken／Beijing／2／97(H9N2)strain was amplified by PCR．The fragment contains EcoR I and ..., Abstract
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2003 Vol. 18 (5): 503-505
Brief Reports
Abstract：Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)has been used as
a com m ercial pesticide for the control of cotton bollworm ．H owever， some kinds of phenolase
(particularly peroxidase)secreted by cotton glands，which generate free radicals，diminish the ..., Abstract
a com m ercial pesticide for the control of cotton bollworm ．H owever， some kinds of phenolase
(particularly peroxidase)secreted by cotton glands，which generate free radicals，diminish the ..., Abstract
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2003 Vol. 18 (5): 506-507
Review
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