A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing ..., Abstract
Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS)and inf~ted by EB virus(EBV),in Order to establish immortalized B lymphoblastoid cell line(B—LCL). B—LCLs were ..., Abstract
Abstract:In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG),gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector ..., Abstract
Abstract:T0 investigate the replication and expression of Hepatitis C virus(HCV)1 a and lb chimeric cDNA in HepG2 cells.HCV l a/ l b chimeric CDNA was used to construct an expression plasmid for the tran sfection of HepG2 cells.HCV protein and HCV genomic RNA an d an ti—genomic RNA in ..., Abstract
Abstract:To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen,the prokaryotic expression vectors pRSET A—S,pRSET A—S—C were constructed respectively.The recombinant expression plasmids were ..., Abstract
Abstract:The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR).Positive vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee,the nucleotide ..., Abstract
To amplify M fragment from unknown Phleboviruses,members of 7 serocomplexes and 8 no complex assigned Phleboviruses,total 42,were chosen and tested.Tl1e M segment amino acid sequences Of 4 Phleboviruses in GenBank were aligned.Conserved regions were selected to design primers. ..., Abstract
Abstract:To develop live—vectored vaccine of Human immunodeficiency virus (HIV-I)without selectable marker,we first constructed a tran sfer plasmid pVI75 with selectable m arkers of neo an d/acZ gene,an d a recombinant plasmid pVI75一Gagpol containing the gagpol gene of Chinese ..., Abstract
Abstract:ChemokiRe receptor CCR5 and CXCR4 are principal coreceptors for HIV Blockage of Human immunodeficiecy virus(HIV)infection can be achieved by engaging CCR5 and CXCR4 with their natural ligands. man herpesvirus 8 encodes three chemokines: vM IP1.vMIP2 and vM IP3.vM Ⅱ’2 has ..., Abstract
Abstract:Nucleocapsid gene of SARS·-associated coronavirus(SARS·-CoV)was obtained by reverse tran scription and polymerase chain reaction from a pateint sufered from severe acute respiratory syndrome(SARS)from Beijing,subsequently cloned into pUCm—T vector.The sequence of positive ..., Abstract
Abstract:A strain of Avian leukosis virus(ALV.J1 subgroup J was isolated from an adult broiler breeder folck.which had a history of myeloid leukosis(ML).in Inner Mongolian.Moreover,another strain of ALV J was isolated from a ML case in Shandong.The two isolates were positive reaction ..., Abstract
Aquareoviruses,which belong to a newly identified genus of the family Reoviridae,are agents infecting all kind of aquatic animals.Grass carp reovirus(GCRV)was isolated from outbreak grass carp fingerling in South China freshwater region,and Threadfin reovirus(1f1W )is a Singapore ..., Abstract
The US2 gene nonessential for viral replication was amplified by polymerase chain reaction(PCR)with DNA from CEF cells infected by MDV vaccine strain CV1988 as template,and was cloned into T-easy vector;Th e expression cassette including GFP gene controlled by CM V promoter and ..., Abstract
In order to develop new vaccine candidate of Equine infectious anema virus(EIAV),env gene of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the downstream of pE/L promoter of pSC65 vector,separately.The resulted recombinant vaccinia ..., Abstract
Abstract:Oedaleus asiaticus entomopoxvirus(DaEPV) contains a factor which enhances the infection of Metarhizium flaroviride in the grasshopper Oedaleus infernalis.When the spheroids of OaEPV were dissolved with 2×SDS spheroid alkaline solution and centrifuged,the enhancing activity ..., Abstract
Abstract:The coding region of superoxide dismutase was amplified by using PCR method from Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus(HaSNPV)C 1 genome.The PCR product was cloned into pGEM —T-easy vector and sequenced.Th e cloned coding region of HaSNPV ..., Abstract
Abstract:Periplaneta fuliginosa densovirus(pfDNV)was found in China at the first time,has been classified into the subfamily Densovirinae in Parvoviridae.However,Which genus that it be longs to has not yet been determined and is the focus of the present work.Recent advances in the ..., Abstract
Abstract:Ectropis obliqua nucleopolyhedrovirus(EoNPV)infects tea looper Ectropis obIiqua speci— tically.The application techniques of EoNPV preparations were studied in this paper.It was suggested that the optimum applied periods were 1~ 2 instar larvae of the 1st,the 2nd,the 5th and ..., Abstract
Abstract: gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT.PCR and then cloned into pGEM—Teasy.Sequence analysis showed that its nucleotide sequence had high identity to a Japan ese isolate of RDV an d contmned high percent of rare codons. l6 gene was expressed m ..., Abstract
To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain,to express E2 protein in BHl(2 l cells,and to analyze the antigenic sites of E2 protein of RV JR23 strain.E2 gene of RV JR23 strain was amplified bv I PCR.The PCR product was cloned into the ..., Abstract
Abstract:The nostructural protein(NS 1)encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus.In this study,NS 1 protein gene of the Chicken influenza virus A/chicken/Beijing/2/97(H9N2)strain was amplified by PCR.The fragment contains EcoR I and ..., Abstract
Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)has been used as a com m ercial pesticide for the control of cotton bollworm .H owever, some kinds of phenolase (particularly peroxidase)secreted by cotton glands,which generate free radicals,diminish the ..., Abstract