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Issue of Virologica Sinica
Article List:
Research Article
Abstract:In this study,we inserted the 5 untranslated region(UTR)of Hepatitis C virus(HCV)
genome into the upstream of the reporter genes of enhanced green fluorescent protein (eGFP)and
luciferase.an d we also constructed the expression vector that Can express the short interfering RNAs ..., Abstract
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Research Article
Abstract:To investigate the suppression effect of Human cytomegalovirus(HCMV)on multipotential
hematopoietic progenitors(CFU—Mix)in vitro,colony forming unit—assay was applied to observe the
effect of HCM V—AD169 strain on CFU—M ix of cord blood.1[11e technique of PCR Was used to ..., Abstract
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Research Article
Abstract:The Hepatitis B virus(HBV)oligochip was made according to the sequence of HBV
polymerase gene.7 genotypes and 4 sero—subtypes of HBV,as well as position rtV173,~.L180,rtlVl21M,
rtV207 in the reverse transcriptase(rt)domain of HBV polymerase,were detected with the chip.45 ..., Abstract
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Research Article
Abstract:To ampUfy Hepatitis C virus(HCV)polymerase sequence and express HCV NS5b protein in
order to,in the next,establish a molecular model of HCV replication.RT—PCR method was used to
amplify HCV NS5b polymerase gene from a Chinese HCV(+1 patient’S serum. e polymerase
fragment ..., Abstract
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Research Article
Abstract:To observe the antiviral activity of Algefacient and anti-inflammatory(AI)against influenza
virus,cell culture technique was used in M DCK cells to get virus inhibitory rate an d virus tiler by MTT
assay an d hemagglitination test.Influenza virus infection model was established ..., Abstract
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Research Article
An eukaryotic vector containing the full—length Hepatitis C virus(HCV)gene,pCi—HCV,was
successfully constructed in this study.After~ansfected into HepG2 cells.it Was revealed to expleSS
HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry as Say ..., Abstract
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Research Article
Abstract:To determine if SARS—associated coronavirus(SARS—CoV)induces apoptosis.we studied the
SARS—CoV infected Vero E6 cells by agarose gel electrophoresis.indirect fluorescent staining for
infection an d Hoechst 33528 staining for nuclei,as well as by flow cytometry.It was found ..., Abstract
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Research Article
Abstract:To isolate and identify the etiologic agent of severe acute respiratory syndrome(SARS),
the throat swabs an d gargle of patients with SARS were collected.We inoculated the specimens onto a
number of cell lines including Vero,Vero E6,M DCK,Hela,Hep一2,HP an d HEL cells.The ..., Abstract
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Research Article
Abstract:From to the nucleotide sequences of haemoagglutinin gene of Influenza virus and Porcine
R rD c and Respiratory Syndrome Virus(PRRSV)VR2332 in GenBank,a pair of primers which
includes the main sequences of haemoagglutinin(HA)gene(33bp)was designed to amplify N gene
(ORF7)of ..., Abstract
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Research Article
Abstract:Three H3 subtype strains of Swine influenza virus(SlV),which were named SSH1,SFJ1 and
SHB1,were isolated from Shanghai,Fujian and Hubei.These isolates could grow and replicate in
chicken emb~o,and the hemagglutinin(HA)values were 1:2 ,1:2 and 1:2 ,respectively.The ..., Abstract
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Research Article
Abstract:The capsid protein gene(vp60)of Rabbit hemorrhagic disease virus(RHDV)isolate NJ85
was amplified and sequenced.vp60 gene of NJ85 was in size of 1 740nt an d encoding 579aa.
Comparison with other Chinese field isolates W X84 an d TP showed that the homology were 92.7% an d ..., Abstract
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Research Article
Mousepoxvirus has been isolated from diferent pathological tissues in deed mouse that
natively infected by virus.In negative stained preparations.the size of the virus particles Was l60~
19Ohm X250~ 300nm and the virus the particle revealed ovoid·shape.Tl1e healthy Blab/C mouse were ..., Abstract
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Research Article
Abstract:Rabbits were injected with a single intravenation of Bovine foamy virus 3026(BFV3026)·
infected cells in the ear and monitored for sera-conversion serologically up t0 l year.Results of the
serological an d virus-rescue assays indicated that all animals were persistently infected ..., Abstract
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Abstract:The chlorfluazuron,a chitinase inhibitor acting on pest like a biological agent was selected
for enhance Helicoverpa口, era nucleopolyhedrovirus(HaNPV)pesticide.The third instar larvae
were infected with the virus mixed witI1 4.2ppm chlofluazuron,the LT50 value Was reduced to ..., Abstract
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Research Article
Abstract:Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress
sod gene of Spotoptera exigua Multi Nucleopoleohedrovirus(SeMNPV)from China has been cloned
an d expressed in E.coli with the activity of SOD being 29 1.1 9U,mL.DNA sequence an alysis ..., Abstract
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Research Article
Abstract:The nucleotide sequence of genome S7 cDNA (AY 1 80908) from Dendrolimus punctatus
cypovirus l Hunan strain(DpCPV—HN )was determined.S7 cDNA consists of 1 501 nucleotides and
encodes a protein of 448 amino acids with a molecular mass of 49.8 kDa.Th e 5 .an d 3 .terminus ..., Abstract
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Research Article
Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera
exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As
pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO ..., Abstract
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Research Article
Abstract:TIle coding region of Oedaleus asiaticus EPV spheroidin gene was obtained by PCR.and this
fragment Was subsequently cloned,seq uenced an d an alyzed.Tlle data shows OaEPV sph coding re on
is 2967bp in size which encodes a ll l l(Da protein.Homology an alysis demonstrated that ..., Abstract
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Research Article
Abstract:Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the
genome DNA of HaSNPV.The PCR products were cloned into pBluescript KS(+)plasmid.The genes
were introd uced into the expression vector pProExHTb.After IFFG induction.the E coli DH5a strain ..., Abstract
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Research Article
Abstract:Here we report the pathogensis process of Helicoverpa armigera larvae infected with
Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)using H.E staining and
immunohistoc hemistry with an tibody against polyhedrin.The fourth instar Helicoverpa armigera larvae ..., Abstract
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Research Article
Abstract:Harpin gene A,B,C and D fragments of Erwinia amylovora(Ea)were synthesized.The full
length Harpin gene was constructed by ligation,and then cloned into pET-23(+).Th e fusion Harpin
protein Was expressed in E.coli BL2 1,purified by His Bind Resin Ni”chromatography and ..., Abstract
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Brief Reports
Abstract:Using nested RT—PCR an d type special primers of HCV,we performed genotype an alysis of
HCV RNA from five representative cities in Hebei Province.Among 168 samples with HCV RNA,104
sera was typelb(61.9%),type 2a account for 22.6% (38/168),and lb/2a mixed—type was ..., Abstract
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Brief Reports
Abstract:According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome
V/ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank, two pairs of
primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR. As
a result, ..., Abstract
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