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Issue of Virologica Sinica
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Research Article
Human immortal epithelial cell line-Hacat cells without expression of CR2(complement receptor 2, CR2) and polymeric immunoglobulin receptor(pIgR) were co-cultivated with marmoset lymphocyte line-B95-8 cells producing EBV for infection by cell-to-cell contacting. After a week, all the B95-8 cells ..., Abstract
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Research Article
To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were ..., Abstract
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Research Article
Eukaryotic expression plasmid pVAXIL2 was constructed by inserting IL-2 gene into the downstream of CMV (cytomegalovirus) promotor in the vector pVAX1. BALB/c mice were co-inoculated muscularly by this plasmid and the nucleic acid vaccine plasmid pVAXGE expressing the HIV-1 (Human immunodeficiency ..., Abstract
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Research Article
Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical ..., Abstract
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Research Article
The nucleocapsid protein (N) gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing. The results ..., Abstract
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Research Article
By using a computational program, Learn Coil-VMF, we determined the two conserved heptad repeat regions (HR1 and HR2) of FIV gp40 protein. The gene of gp40 subdomain including HR1 and HR2 was constructed with PCR method. The HR1 and HR2 were subsequently expressed and purified as a single chain ..., Abstract
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Research Article
10 influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned ..., Abstract
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Research Article
Using the total mRNA in the lymphocyte in chicken blood as the template, the mature protein gene of interferon γ-interferon was cloned and amplified through the reverse transcription polymerase chain reaction. The gene was then ligated to pRLC to establish the gene’s non-fusion expressing vector. ..., Abstract
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Research Article
In this paper we reported the electron microscope(EM) examination of “Trembling disease” virus in Eriocheir sinensis. The virions were globular, with envelope and spikes, and about 150nm of diameter. The purified nucleic acid was treated with 8mol/L urea and the viral nucleic acid was transferred ..., Abstract
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Research Article
A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later ..., Abstract
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Research Article
Using Bac-to-Bac system, the transfer vector pFCP with full-length cry1Ac10 gene of Bacillus thuringensis drived by the very late expressed gene ph promoter and intact polyhedra gene was constructed successfully. The recombinant virus vFcph , with complete polyhedra and cry1Ac10 gene, was obtained ..., Abstract
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Research Article
By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus(SeNPV)has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin ..., Abstract
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Research Article
A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0μg/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% ..., Abstract
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Research Article
The results of UV and sunlight on the activity of freshwater cyanophage PP are presented in this paper. Both UV-A and UV-B resulted in significant inactivity of cyanophage, though the effect was different. The outdoor performance showed that the day inactivity rates of cyanophage by sunlight in ..., Abstract
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Research Article
It is very important to optimize the operating conditions of virus concentration from water body for evaluating virus’s pollution and removing efficiency. In this paper, an optimizing method of virus concentration from water body was established. It was to adopt the way of throwing clay of ..., Abstract
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Brief Reports
From the conserved regions of the reported nucleotide sequences of whitefly-transmitted geminiviruses (WTGV), a pair of degenerate primers was designed to anneal to the conserved sequence. The tomato samples infected geminivirus-like from Guangdong were detected by PCR. The results showed that a ..., Abstract
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Brief Reports
To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by ..., Abstract
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Brief Reports
To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed ..., Abstract
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Brief Reports
Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV ..., Abstract
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Brief Reports
To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays. ..., Abstract
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