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Virologica Sinica, 19 (1) : 14-17, 2004
Research Article
Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province
1. Medical College of Zhejiang University, Hangzhou 310009, China
2. Zhejiang Center for Disease Prevention and Control
Hangzhou 310009, China
 Correspondence:
(213.42KB)  
Abstract
Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.
  Published online: 20 Feb 2004
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