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Virologica Sinica, 19 (1) : 14-17, 2004
Research Article
Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province
1. Medical College of Zhejiang University, Hangzhou 310009, China
2. Zhejiang Center for Disease Prevention and Control
Hangzhou 310009, China
Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.
  Published online: 20 Feb 2004
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