Hello guest, Register  |  Sign In
Issue of Virologica Sinica
Article List:
Research Article
To investigate the effect of interleukin-18 (IL-18) DNA immunization on immune response induced by Human immunodeficiency virus 1(HIV-1) nucleic acid vaccine (DNA vaccine), the recombinant expression vector pVAX1-IL-18 was constructed by inserting the IL-18 gene into the eukaryotic expression ..., Abstract
Free PDF (192.24KB)
Research Article
Hepatitis B virus core antigen protein gene (C gene) was expressed in Saccharomyces cerevisiae and the products (rHBcAg particles) were purified from crude lysate of the yeast by three steps: Sephrose CL-4B chromatography, sucrose step-gradient ultracentrifugation, and CsCl-isopycnic ..., Abstract
Free PDF (224.05KB)
Research Article
CD40L gene can act as a genetic adjuvant. The objective of this study was to test the effectiveness of CD40L-expressing plasmid on the immune response to influenza A/PR/8 virus hemagglutinin (HA) DNA vaccine. To achieve this, we constructed plasmid DNA encoding murine CD40L and coimmunnized the ..., Abstract
Free PDF (251.55KB)
Research Article
SHIV-KB9 chimeric virus was constructed by two constructs representing the 3’ and 5’ fragments of the viral genome. This full-genome SHIV-KB9, with large size and long terminal repeat sequence (LTR), could be maintained stable in E.coli JM109 strain in the cultivation condition of 30℃ and ..., Abstract
Free PDF (199.80KB)
Research Article
A 3.76 kb genome DNA fragment, containing the complete coding sequences of UL31, UL32, UL33 and UL34 genes and the partial coding sequences of UL30 and UL35 genes of Pseudorabies virus (PRV) Ea strain, was amplified by PCR and sequenced. The results show that the G+C contents of UL31, UL32, UL33 ..., Abstract
Free PDF (235.79KB)
Research Article
Two recombinant replication-defective human adenovirus serotype 5 vector containing foot-and-mouth disease virus (FMDV) serotype O capsid P1-2A, viral 3C protease and 3D polymerase coding region were constructed using the method of homologous recombination in bacteria. Capsid P1-2A, 3C protease and ..., Abstract
Free PDF (205.81KB)
Research Article
In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase ..., Abstract
Free PDF (187.34KB)
Research Article
In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR ..., Abstract
Free PDF (190.76KB)
Research Article
A pair of primers was designed according to the sequence of ISKNV mcp gene, and a DNA fragment about 1400bp was amplified by PCR using the designed primers. The amplified product was cloned into the pGEM-T Easy Vector. Amino acid hydrophilic analysis indicated that the amino acids residue between ..., Abstract
Free PDF (215.63KB)
Research Article
The strain purified from a baculovirus expressing recombinant human interleukin-12 by plaque selection was serially and undilutedly passaged up to 55th generation in Spodoptera frugiperda 9(Sf9) cells. Intracellular viral (ICV) DNAs were extracted from the infected Sf9 cells of P15, P25, P35, P45 ..., Abstract
Free PDF (256.33KB)
Research Article
Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was ..., Abstract
Free PDF (126.42KB)
Research Article
Based on reported Cymbidium mosaic virus(CyMV)and Odontoglossum ringspot virus(ORSV)genomic cDNA sequences, two pairs of PCR primers for viral coat protein gene with expected ampliffied sizes of 784bp and 604bp were designed respectively, and RT-PCR was performed with the total RNA as templates ..., Abstract
Free PDF (193.17KB)
Research Article
Rice black streaked dwarf virus (RBSDV) infecting stages were viewed in maize vein cell during its different infection period by electron microscope. It was found that the RBSDV particles infected in the maize cell appeared at first in the cytoplasm near the cell wall, some single virus particles ..., Abstract
Free PDF (299.13KB)
Research Article
Prions are unprecedented infectious pathogens that cause a group of invariably fatal neurodegenerative diseases by an entirely novel mechanism. Prion diseases result from abnormally folded isoform (PrPSc) of the cellular prion protein (PrPC). In order to investigate the molecular mechanisms of the ..., Abstract
Free PDF (247.89KB)
Research Article
The efficiency of adsorption and removing Poliovirus 1(PV1) and bacteroides fragilis phage(B.fp) by nature and modified sand in the conditions of different environment pH, various mental salts and concentration variation, exist of humic acid in tap and distilled water were studied in this paper. ..., Abstract
Free PDF (254.47KB)
Brief Reports
BLCAP is a potential gene for suppression of cervical carcinoma, which was found by analysing the cervical carcinoma specimen with the oncogene and anti-oncogene cDNA microarray. Basing on the bioinformatical analyses, we try to predict the function of blcap gene. The results show that there are ..., Abstract
Free PDF (141.62KB)
Brief Reports
Full E2 gene fragment of Classical swine fever virus (CSFV)shimen strain was amplified by RT-PCR method, then a specific fraction -the A, D antigenic domains- , which encodes protein with good antigenicity and suitable for being expressed in E. coli with high level, was amplified respectively by 2 ..., Abstract
Free PDF (147.64KB)
Brief Reports
Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. ..., Abstract
Free PDF (115.09KB)
Brief Reports
To study the distribution characteristics of Hepatitis B virus (HBV) genotypes in groups of the Zhuang nationality of Baishe in Guangxi, the PCR sandwich hybridization-ELISA technique was used to determine the genotypes in 30 patinets of Zhuang nationality with hepatitis B. Geontype B, C, D and non ..., Abstract
Free PDF (91.36KB)
Brief Reports
Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product ..., Abstract
Free PDF (162.58KB)
Review
Review
Review
Review
Review
Recent Issues:
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved