Hello guest, Register  |  Sign In
Issue of Virologica Sinica
Article List:
Research Article
Partial overlapping primers were designed based on the severe acute respiratory syndrome coronavirus (SARS-CoV) sars7a gene and were chemically synthesized. The sars7a gene fragment was obtained by two-round of PCR and this fragment was used as template for a further round of PCR by using a pair of ..., Abstract
Free PDF (600.15KB)
Research Article
The purpose of this study is to establish a new method which HCV RNA can be used to detect sensitively and specifically through efficient single strand RNAs(ssRNAs) amplification. The sera from the chronic Hepatitis C patients were used as specimens. Single step reverse transcription-PCR (SRT-PCR) ..., Abstract
Free PDF (887.20KB)
Research Article
In this paper, based on the especial interaction among purificated Dengue 2 virus, monoclon-
al antibody against Dengue virus, virus receptor, and Protein-G-Agrose; one non-glyprotein, which molecular weigt is approximately 35kDa, has been identified to be the receptor of Dengue 2 virus on the ..., Abstract
Free PDF (826.18KB)
Research Article
The HPV E7 variant shares the same coding sequence with the wild type E7 except that the 43rd cordon was mutated from CAA to TAA. The recombined plasmid pcDNA3.1-(by) E7 DNA and pcDNA3.1-(ys)- E7 DNA, were constructed by molecular clone technology, and then injected into mice intradermally ..., Abstract
Free PDF (182.95KB)
Research Article
In order to study the immune responses of Hantaan virus envelope glycoproteins G1 and G2 in adenovirus expression system, G1 and G2 gene were amplified from Hantaan virus 76-118 and cloned to the plasmid pAdTrackCMV. Positive clones pAdTrackCMV-G1 and G2 were determined with restriction enzyme ..., Abstract
Free PDF (643.13KB)
Research Article
vp2-4-3 gene of very virulent infectious bursal disease virus strain SH95 (vvIBDV) was double enzyme digested and cloned into eukaryotic expression vector pALTER-MAX by cohesive ends. The recombinant plasmid was identified by enzyme-digestion, which showed that the gese was cloned into pALTER-MAX ..., Abstract
Free PDF (866.44KB)
Research Article
The RNA S7 encoding serogroup-specific antigen VP7 of bluetongue virus (BTV) was cloned and inserted into pBAD/Thio TOPO vector. The recombinant plasmid was identified by restriction analysis, PCR and sequence. The results of SDS-PAGE and Western immunoblotting revealed that the VP7 was expressed ..., Abstract
Free PDF (937.68KB)
Research Article
To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing ..., Abstract
Free PDF (133.75KB)
Research Article
Four field strains of Infections bursal disease viruses (IBDV) isolated in China during 1997~1999 were compared for their pathogenity and sequences in high variable (hv) region of vp2 genes. It was indicated that GX8/99 was a very virulent IBDV strain (vvIBDV), pathogenicity of other 3 strains was ..., Abstract
Free PDF (212.73KB)
Research Article
The recombinant plasmid pETN was transformed into E.coli BL21 (DE3) host cell and the expression product-recombinant nucleocapsid protein of PRRSV was obtained under the optimized condition of host cell cultivation and IPTG induction. Consequently, the expression product was purified by means of ..., Abstract
Free PDF (588.08KB)
Research Article
The long terminal repeat (LTR) of Reticuendotheliosis virus (REV) was amplified by PCR technique and cloned into pUC18 vector at the sites of EcoRI and SacI, and the BGH polyadenylation signal sequence was cloned at the sites of Sph I and Hind Ⅲ as a terminor. The positive clone was named pUC-LTR ..., Abstract
Free PDF (469.08KB)
Research Article
In this study, the DNA polymerase genes of 9 different pathotypes strains were compared, to analyze the relationship between the pathotype and MDV’s DNA polymerase gene. These strains included four international reference strains—vaccine strain CVI988, virulent strain GA, very virulent strain Md5 ..., Abstract
Free PDF (0KB)
Research Article
The nucleotide sequences of six strains named T1, T2, T3, T4, T5 and T6 of FMDV type O were determined. RNA of the six cell-adapted virus strains were extracted by single-step method of RNA isolation, reverse-transcripted and amplified by PCR, respectively. PCR products were cloned and sequenced. ..., Abstract
Free PDF (511.21KB)
Research Article
In the study, we designed an antisense RNA targeting at the highly conserved sequence (26880-27184nt)of Transmissible gastroenteritis virus of swine,(TGEV). The fragment cloned by PCR was recombined with retrovirus expression vector, pLXSN, and transfected into packaging cell line PA317 with ..., Abstract
Free PDF (826.93KB)
Research Article
Mx protein is one of the classes of antiviral proteins induced by type I interferons (IFN). A full-length cDNA of mandarinfish (Siniperca chuatsi) Mx gene was obtained using reverse transcription polymerase chain reaction (RT-PCR) and RACE (rapid amplification of cDNA ends) polymerase chain ..., Abstract
Free PDF (813.05KB)
Research Article
The conotoxinlike (ctl) gene is a small gene in the genomes of several baculoviruses. The biological function of baculoviral ctl gene remains unknown. In this report, we generated a recombinant AcMNPV(AcBac-ph-ctl) which contains the ctl gene from BusuNPV using AcMNPV bacmid system. The ..., Abstract
Free PDF (901.37KB)
Research Article
The coat protein (CP) gene of the Beijing isolate of Wisteria vein mosaic virus (WVMV-BJ) was amplified by RT-PCR, and ligated to the expression vector pET22b(+). The recombinant plasmid pET-WVMVCP was transformed into E. coli BL21(DE3) and then induced by IPTG. It was shon that the CP gene was ..., Abstract
Free PDF (702.87KB)
Brief Reports
The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region were tested by ELISA for the presence of IgG antibodies against HEV. The ..., Abstract
Free PDF (115.66KB)
Review
Review
Review
Review
Review
Review
Recent Issues:
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved