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Issue of Virologica Sinica
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Research Article
To develop the pathogenesis of Hantavirus infection, the differentially expressed signal trans- duction-associated genes of integrin in Hantavirus infected cells were investigated by using cDNA microarray. The primary human embryo pulmonary fibroblasts (HEPFs) were infected with Hantaan 76-118 ..., Abstract
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Research Article
A Vero-E6 culture system was developed to assay Hepatitis C virus (HCV) replication by plasmid (pHCV) transfection,which contains a T7 promoter at the 5’end, full-length cDNA of the HCV genome and a T7 terminator. To facilitate high-level transcription of HCV RNA, Vero-E6 cells were transfected ..., Abstract
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Research Article
To study the enzymatic activity,the recombinant HCV NS3 protein with both protease and helicase activity was expressed and purified. The nonstructure gene 3 (NS3) of HCV was amplified and inserted into plasmid pPIC9 and the recombinant plasmid pPIC-NS3 was transformed into Pichia pastoris strain ..., Abstract
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Research Article
The pregenomic RNA, serves as a template for reverse transcription and encodes the reverse transcriptase for Hepatitis B virus (HBV) replication, has the energetically flexible secondary and tertiary structures, which may reduce the accessibility of ribozyme. To identify accessible target sites for ..., Abstract
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Research Article
Recombinant plasmid pCNS2 was constructed by inserting Hepatitis C Virus full-length NS2 gene between Xho I and Hind III sites of an eukaryotic expression vector pCDNA3.1(-). The recombin- ant plasmid pCNS2 was transfected to Huh-7 cells with LipoVecTM and HCV NS2 mRNA and protein produced in ..., Abstract
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Research Article
Full-length NS5A gene of Hepatitis C virus(HCV) was amplified by PCR, using the plasmid pBRTM/HCV 1-3011 containing HCV full-length open reading frame(ORF)as template, and cloned into the eukaryotic expressing plasmid pcDNA3.1(-)by DNA recombination technique. The recombin- ant vector was ..., Abstract
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Research Article
Entire ORF of Hepsin was isolated from adjacent live tissue of HCC with RT-PCR. Hepsin gene was inserted into expressing vector pCMV-tag-HS. The recombinant vector pCMV-tag-HS was co-transfected into liver tumor line Hep G2.2.1.5 with plasmid encoding HBx protein. The results indicated that ..., Abstract
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Research Article
Using BTV-HbC3 to infect tumor cells including human lung cancer SPC-A-1 cells, human cervical carcinoma HeLa cells, human astrocytoma U251 cells, mouse-derived astrocytoma C6 cells and human embryonic lung cells and observe the cytopathic effects(CPE); then using transmission electron microscope ..., Abstract
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Research Article
NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5% ..., Abstract
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Research Article
With the reverse transcription polymerase chain reaction (RT-PCR), the DNA sequence encoding the cattle’s interferon-gamma (BovIFN-γ) and the signal peptide was amplified, from the total RNA of the lymphocytes stimulated with concanavalin A in the peripheral blood of bovine, which was then cloned ..., Abstract
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Research Article
To investigate Hepatitis E virus,(HEV) infection in swine in Xinjiang and the extent of genetic variation among Chinese swine HEV strains, seventy pig fecal samples from an anti-HEV positive farm in Xinjiang were tested for the presence of HEV RNA by reverse transcription nested polymerase chain ..., Abstract
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Research Article
The porcine IFN-βgene was amplified by polymerase chain reaction. The amplified fragment was cloned into pGEM-T-easy vector and then sequenced. The result indicated that the cloned gene was a complete porcine IFN-βgene with the codes for signal peptide ,which had the identities of 100% with the ..., Abstract
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Research Article
pp24 gene was amplified from genomic DNA of Marek’s disease viruses (MDV) Md11 strain by polymerase chain reaction(PCR) and then cloned into pGEX-6P-1 according to the right open reading frame(ORF). The recombinant plasmid was transformed into E.coli BL21 strain for expression with the induction ..., Abstract
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Research Article
To establish a technique for preparing rabies vaccine by perfusion culture on microcarriers in bioreactor, a 5 litre bioreactor was filled with 199 cell culture medium which contains 10g/L of microcarriers. Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of ..., Abstract
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Research Article
To improve the safety of recombinant vaccinia virus,a non-replicating vaccinia recombi- nant co-expressing rabies virus glycoprotein(RG) and nucleoprotein(RN) without a selectable marker was reconstructed. LacZ between fragment C and fragment K in non-replicating recombinant vaccinia virus ..., Abstract
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Research Article
The PCR product of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus HaSNPV fp25k gene was cloned into a prokaryotic expression vector pProEXHTb. After inducing with IPTG, the fp25k gene was successfully expressed in E.coli DH5α. The expression product was 32 kDa. This protein was ..., Abstract
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Research Article
It is very important to understand the laws of Tobacco mosaic virus(TMV) inactivation under different water bodies and temperatures. Choosing the typical plant virus TMV as a model virus, the inactivation dynamics of it in Min River water, tap water, super-pure water, life sewage and sterile sewage ..., Abstract
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Research Article
5 strains of verocytotoxigenic (VT) bacteriophage were isolated from E.coli O157 strains, cow feces, chicken feces, sewage and purified by two-layer agar assay. Gene vt2 was amplified by PCR in these phage isolates. The plaques of these phages were 0.5-2mm in diameter and the titre of phages with ..., Abstract
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Research Article
With a pair of primers and the E. coli chromosome DNA as the template, an intact recA gene was obtained by PCR. The recombinant plasmid was constructed by ligating the PCR product and pUC18 in vitro and then transformed into E. coli DH5α and E. coli k12(λ+), respectively. The plasmid pUR4 ..., Abstract
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Brief Reports
Porcine reproductive and respiratory syndrome virus(PRRSV) isolates identified in samples from 50 field cases originated from eastern China herds were studied. ORF5 gene of PRRSV isolates was amplified by reverse transcript polymerase chain reaction(RT-PCR) and restriction fragment length ..., Abstract
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Brief Reports
The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the ..., Abstract
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Brief Reports
Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower ..., Abstract
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Brief Reports
A novel procedure was used for cloning large adenovirus genome fragment by the homologous recombination in E.coli strain BJ5183. The 11.2Kb downstream fragment of the CAV-2 strain YCA18 genome was cloned by homologous recombination, the 1029bp left end and the 970bp right end of this fragment were ..., Abstract
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Brief Reports
In order to study the feasibility of gene chips technology in the detection of HBV mutation associated with lamivudine, we detected the mutation of HBV in peripheral blood of 30 patients treated with lamivudine for at least half a year by gene chips. The result was compared with that from direct ..., Abstract
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