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Issue of Virologica Sinica
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Research Article
Coding sequences of Transfusion transmiffed virus(TT virus or TTV) ORF2 was amplified by PCR, using plasmid pET-His-TTV2 containing the full-length ORF2 of TTV as template. The amplified fragment of 606bp was cloned into plasmid pEGFP-N1 and GFP-VP2 fusion protein was expressed. The recombinant ..., Abstract
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Research Article
Abstract:To analyze the diferent expression proteins of MT4 cells infected witl1 HIV 1 IIIB strain.The proteins of MT4 cells from infected and Uninfected HIV 1 IIIB were separated bv 2DE and the gels were an alyzed with software Image Master 2D Elite 3.01 to find out the diferent spots.The ..., Abstract
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Research Article
In order to provide some data for the study on T cell epitopes of Hantaan virus nucleocapsid protein, we constructed EBV-transformed B lymphoblastoid cell line (BLCL) from HFRS patients’ PBMC. We inserted HTNV S and 5’ and 3’terminals of S gene segment into a eukaryotic expression vector pcDNA ..., Abstract
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Research Article
In order to analyze the gene characterization of Human immunodeficiency virus-1(HIV-1) prevalence in Henan Province, a HIV-1 strain, named CNHN24, was isolated from one HIV-1 infected blood donor. The proviral DNA was extracted from productively infected PBMCs (peripheral blood mononuclear cells) ..., Abstract
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Research Article
To understand the characteristics of the ns1 gene of Avian influenza A virus H5N1 and to prepare NS1 protein in scale, viruses were cultivated in embryonated hen eggs and virion RNA was extracted from allantoic fluid. The complete ns gene was amplified by RT-PCR and analyzed. Sequence analysis ..., Abstract
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Research Article
According to the published genomic sequence of Porcine circovirus(PCV2), a pair of specific primers was designed to amplify the complete genome of PCV2 from tissues of piglets with PMWS. Five complete genomes of PCV2, named Fujian PCV, Guangxi PCV, Hainan PCV, Hunan PCV and Shandong PCV, were ..., Abstract
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Research Article
A pair of primers was used in cloning and sequence analysis of vp4 gene from Porcine rotavirus JL94 isolated in China. According to the idea that 5’ segments of VP4 (1bp~750bp) principally control the activity of vp4 gene, a pair of another primers was used in cloning and obtained major antigen ..., Abstract
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Research Article
According to the published genomic sequences of Porcine reproductive and respiratory disease syndrome virus (PRRSV),Porcine parvovirus (PPV) and Porcine circovirus type 2, primers were designed and RT-PCR, PCR and multiplex PCR were set up for the detection of PRRSV, PPV and PCV, respectively. 127 ..., Abstract
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Research Article
To study the interaction among Jembrana disease virus, Bovine immunodeficiency virus, Bovine Foamy Virus and Bovine Leukemia Virus, a series of plasmids were constructed with their LTR and luc report gene, respectively, and transactivator expression plasmids of these four viruses were also ..., Abstract
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Research Article
According to the nucleotide sequence of Porcine reproductive and respiratory syndrome virus (PRRSV), two pairs of primers were designed and used to amplified the orf7 and orf5 genes by RT-PCR, respectively. By using the EcoRI、SpeI and Hind Ⅲ sites, both genes were cloned into pMD-18T vector in ..., Abstract
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Research Article
Spike (S) gene of Canine coronavirus giant panda’s isolate (CCV strain DXMV) was amplified and cloned into pGEM-T for sequencing. The complete length of spike gene of strain DXMV was 4362 bp, which encoded 1453 amino acids. The initiative 18 amino acids were signal peptide. The homologies of ..., Abstract
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Research Article
One pair of primers was designed and synthesized based on the Haemagglutinin (H) protein gene Sequercein the Onderstepoort strain of Canine distemper virus(CDV). The templates were produced from the reverse transcription reaction, total RNA was isolated from the Vero cells infected with the Nanjing ..., Abstract
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Research Article
A strain of avian influenza virus was isolated from lung tissue in 2004. It was determined to be a Avian influenza virus by ELISA and electron microscopy. HA and NA gene was amplified by RT-PCR and sequenced. By the analyses of HA and NA gene sequences, the isolate was an H5N1 subtype of avian ..., Abstract
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Research Article
According to the Granulin gene’s 12 highly conservative nucleotides (TATAAGGAATTT) upstream from the protein intiation codon and the sequence of the Pieris brassiae granulovirus (PbGV) granulin gene, primers were designed to amplify the Pieris rapae granulosis virus (PrGV) granulin gene. The ..., Abstract
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Research Article
Autographa californica nucleopolyhedrovirus (AcMNPV) is the typical member of nucleopolyhedrovirus of Baculoviradae, a circled and double stranded large DNA virus. It’s also one of the most widely studied baculoviruses that turns out to be environment sound pesticide and a powerful vector of ..., Abstract
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Research Article
Genomic DNA of Orgyia ericae single-nucleocapsid nucleopolyhedrovirus (OrerSNPV) was digested with the restriction enzyme EcoRI and the fragments were cloned into the plasmid pUC19 to build a library for sequencing. An ORF encoding the LEF-2 was identified from the cloned EcoRI-M (3.0kb)and ..., Abstract
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Research Article
The fragment of plasmid pCMV-tag-2B excluding Cytomegalovirus(CMV) promoter sequence was obtained by restriction enzyme digestion. A pair of primers was synthesized to amplify a fragment of the H1 promoter according to reference. The fragment of H1 promoter was amplified and inserted into the ..., Abstract
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Brief Reports
Using a pair of specific primers designed according to the relevant nucleotide sequence of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)VL2332 strain, Lv strain and Classical Swine Fever Virus(CSFV) C strain from GenBank, RT-PCR method was established to detect these two viruses at the ..., Abstract
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Brief Reports
To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. ..., Abstract
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Brief Reports
In order to explore the relationship between Hepatitis B virus infection(HBV) and hepatocellular carcinoma (HCC), a case-control study was conducted based on the diagnosed HCC patients in Wuhan city from January 1, 2000 to July 31, 2003. The data obtained from 313 cases and 313 controls were first ..., Abstract
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Brief Reports
In this experiment the Fowlpox virus (FPV) antibody of two groups of chickens were tracked for 20 weeks by indirect enzyme-linked immunosorbent assay (ELISA). A group of chickens were artificial infected. B group were the control of healthy chickens. The results indicated that the positive antibody ..., Abstract
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