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Research Article
We analyzed and verificated the B-cell epitope of the L1 capsid protein of the Human papilloma virus type 6, and made it the base of producing new subunit peptide vaccine. Goldkey and PC/ Gene computer program were used to colligate and analyze the B-cell epitope of HPV6 L1. These peptides were ..., Abstract
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Research Article
To create the pathogenicity model of severe acute respiratory syndrome (SARS) on macaca rhesus, the macacu rhesus was infected with SARS coronavirus (CoV) was detected in different samples coming from macaca rhesue in different days by virus isolation, immunoflurescence assay, pathological ..., Abstract
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Research Article
To understand the relationship between the chronic hepatitis B virus infection and the primary hepatocellular carcinoma (PHC), we adopted the method of retrospection and carried out analysis by comparing the markers of Hepatitis B virus (HBV M) and the result of liver function testing of the 328 ..., Abstract
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Research Article
Although severe diseases may be caused by respiratory syncytial virus (RSV) infection in the world, no efficacious vaccine against this virus is licensed. In an effort to seek recombinant protein antigens that may be used in the future vaccine development, we constructed a series of expression ..., Abstract
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Research Article
The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups ..., Abstract
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Research Article
The recombinant retroviral vector pBABE-puro-E1E2 was constructed by inserting the full-length HCV e1e2 gene of H77 strain into pBABE-puro. Both the recombinant retroviral vector and the pVSVg plasmid were transfected into eukaryotic cells 293T by calcium phosphate transfection method. And then ..., Abstract
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Research Article
vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid ..., Abstract
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Research Article
White spot syndrome virus ORF220 encodes a protein homologous to the gp130 receptors of eukaryote. prcvious studies showed that this protein was a minor structural protein. In this paper, the ORF220 was cloned into the pFastBacI fused with EGFP gene and co-transfected with AcBacmid into DH10B ..., Abstract
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Research Article
In order to define if similar cytokines take part in transactivation of Jembrana disease virus and Human immunodeficiency virus-1 Tat protein, two truncated Tat proteins, hTat47 and jTat70, which contained intact activation domains but lacking binding domains, were constructed. We also constructed ..., Abstract
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Research Article
Here we report that the major antigenic protein VP2 of very virulent Infectious bursal disease virus (vvIBDV) was secretively expressed with high level in yeast (Pichia pastoris) expression system. Primers were designed to amplify the previously cloned vvIBDV-vp2 gene from the plasmid pMD18-T- VP2. ..., Abstract
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Research Article
Two pairs of primers were designed according to the sequences of envelope genes, vp19 and vp28 of White spot syndrome virus (WSSV) in the GenBank. The two DNA fragments about 370bp and 630bp amplified by PCR were linked to the EcoR I restriction endonuclease site and cloned into E.coli expression ..., Abstract
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Research Article
Structural and envelope glycoprotein E2 (gp55) of Classical swine fever virus (CSFV) is the most antigenic protein being responsible for eliciting neutralizing antibodies and conferring protective immunity. Infection of cells with CSFV is mediated by the interaction of E2 and Erns with the cell ..., Abstract
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Research Article
Based on the fact that the envelope glycoprotein E2 which can protect swine from virulent attack of Classical swine fever virus(CSFV) has two structural antigenic domains B/C and A/D, a pair of specific primers was designed to amplify the gene fragment encoding A/D antigenic domain of E2 protein. ..., Abstract
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Research Article
Thymidylate Synthase (TS) is an enzyme involved in making one of the major building blocks for DNA deoxythymidylic acid (dTMP), and plays a critical role in cell growth and division and is overexpressed in the majority of cancers. Lymphocystis disease virus (LCDV) belong to iridoviridae, and the ..., Abstract
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Research Article
On the basis of construction the transfer vector pgEI-GFP of Pseudorabies virus SH strain BHK-21, which was infected with PRV-SH for 1-2h, were tansfected with the complex of pgEI-GFP and DOTAPA deletion mutant was selected and purified 3-4 times in BHK-21 cell through GFP. In the research here, we ..., Abstract
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Research Article
In order to effectively differentiate between gene-deleted vaccine and wild-type isolates of Pseudorabies virus(PrV), a PCR method, based on sequences of gE and gI of the PrV genome, was established after selection of the optimal reaction conditions. By applying this technique, the 848bp gene ..., Abstract
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Research Article
In this study, the major antigenic region of spike (S) gene of Canine coronavirus giant panda’s isolate (CCV strain DXMV) was expressed in Pichia pastoris. The S1 gene fragment encoding major antigenic region of S protein was amplified and cloned into T vector, then subcloned into pPICZαA for ..., Abstract
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Research Article
Hypervariable region of vp2 gene of six different IBDV (Infectious bursal disease virus) isolates, which were isolated from Jiangsu province, was amplified by RT-PCR and sequenced. Furthermore, sequences of six IBDV isolates were analyzed by ClustalX and Phylip3.5. It was suggested that three ..., Abstract
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Research Article
One anti-idiotypic monoclonal antibody(a-Id Mab)was raised directed to both rabbit and chicken IgG against Avian influenza virus(AIV). The Mab was able to inhibit the binding of AIV to anti-AIV IgG and specific in binding anti-H9-subtype-AIV IgG. Another experiment demonstrated that the Mab might ..., Abstract
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Research Article
The nucleotide sequence of Dendrolimus punctatus Wenshanensis cypovirus genomic segment 8 (S8) has been determined. S8 consists 1332 nucleotides and encodes a putative protein of 390 amino acids with a molecular mass of approximately 44kDa (P44). Then, the fragment containing p44 gene was inserted ..., Abstract
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Research Article
To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of ..., Abstract
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Brief Reports
Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a ..., Abstract
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Brief Reports
In this essay the destructive rates of HBsAg, the surface antigen of HBV, exposed to different light sources and photocatalysed time on both TiO2 ceramic plate and Ni net were investigated, the effect of anti-HBV of TiO2 was discussed under different light condition.The result demonstrated that ..., Abstract
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