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Issue of Virologica Sinica
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Research Article
The full-length gene of membrane protein ( M )of SARS coronavirus (SARS-CoV) was cloned using RT-PCR from the Vero-E6 cells infected with SARS-CoV (GD322 strain) and inserted into the multi-cloning site of the pPICZαB vector. The recombinant plasmid was transtormed into P. pastoris strain X-33 by ..., Abstract
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Research Article
Using a pair of specific primers designed according to the nucleotide sequence of Japanese encephalitis virus and Hameoaggulatinin of Human influenza virus from GenBank, the gene of JEV E protein’s main antigenic domain including the sequence of Human influenza virus ha gene was amplified with PCR ..., Abstract
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Research Article
Using a pair of specific primers designed according to the nucleotide sequence of Japanese encephalitis virus and Hameoaggulatinin of Human influenza virus from GenBank, the gene of JEV E protein’s main antigenic domain including the sequence of Human influenza virus ha gene was amplified with PCR ..., Abstract
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Research Article
To investigate the effect of interleukin-12 DNA immunization on immune responses induced by HIV-1 nucleic acid vaccine(DNA vaccine)and to explore new strategies for therapeutic HIV-1 DNA vaccine, Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for ..., Abstract
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Research Article
VP1 is the major structural protein of the human polyomavirus BK and can be used for the generation of virus-like particles (VLP) in vitro by using recombinant baculoviruses. To determine the role of positively charged residues R-281, R-285, K-288, R-290, R-292, K-293, R-294, and K297 at the C ..., Abstract
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Research Article
Bacterial and synthetic DNA containing CpG dinucleotides in specific sequence contexts are being tested as immune adjuvants in many disease settings. To study the immune responses induced by the CpG-enhanced plasmid DNA vector encoding hantavirus nucleiocapsid protein. BALB/c mice were immunized ..., Abstract
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Research Article
Non-structural and structural genes of Goose parvovirus (GPV) HG5/82 strain were amplified using polymerase chain reaction (PCR) method and cloned into the pMD18-T vector, respectively. The two fragments were sequenced and analyzed with reference GPV strains and Muscovy duck parvovirus(MDPV) ..., Abstract
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Research Article
Inhibitory effects of nitric oxide (NO) on Porcine parvovirus (PPV) infection on porcine kidney (PK-15) cells were studied in the paper. The results showed that production of NO was effectively induced in cell cultures treated with S-nitroso-N-acetylpenicllamine (SNAP) or L-arginine and replication ..., Abstract
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Research Article
The matrix and the capsid are main structural proteins of Bovine immunodeficency virus (BIV) and play important roles in virus life cycle. In order to analyze these two proteins in vitro, we cloned these two genes into the prokaryotic expression vector pTXB and expressed in E. coli BL21(DE3) in a ..., Abstract
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Research Article
faecal samples of dogs collected from August 2003~January 2004 were tested for the presence of Canine coronavirus (CCV) using a nested-PCR with conserved primers for the M gene.18 out of 43 diarrhea feces from dogs housed singly of Nanjing city were CCV positively, and the CCV detectable rate were ..., Abstract
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Research Article
The genome of BFDV strain HBYM02 isolated from Hubei province was sequenced and analyzed. The sequence suggested BFDV isolated from different hosts belonged to one genotype. A phylogenetic tree based on the genomic nucleotide sequences of seven isolates was constructed. This tree showed two main ..., Abstract
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Research Article
An infectious bursal disease virus (IBDV), isolated from Dongying city in Shandong province, was propagated several generations in SPF embryos and then turned to cell culture. The chickens were immunized by 20th, 21st, and 25th generation cell viruses in different doses at the age of 7 d and 14 d ..., Abstract
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Research Article
Based on t he infectious clone p FD328 wit hin EIAV f ull2lengt h genome , and according to the variation of structural genes during vaccine preparation , several chimeric infectious clones involved in gag and env genes , modified by overlap PCR site2 directed ..., Abstract
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Research Article
A pair of primers was designed to amplify vp4 gene of major antigen site (1-756 bp) of JL94 isolate. The sequence was analyzed in comparison with the VP4 amino acid sequences of two reference porcine rotavirus. The amino acid sequences were 96.43% and 67% correspondingly. Notably, the most ..., Abstract
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Research Article
Variations in the amino acid sequence of Foot-and-mouth disease virus (FMDV) structural proteins are the molecular basis for the antigen diversity of the virus. Majority of antigenic sites for the virus neutralization are present on VP1, the major immunogenic protein. However, a few conformational ..., Abstract
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Research Article
In order to investigate the antiviral activity of 112 kinds of nanomaterials and give theoretical gist for developing new antiviral nanomaterials, we used various nanometer catalysts inhibitory to Parainfluenza virus, Herpes simplex virus types 1, Adenoviruses types 1 and 5 in given time and ..., Abstract
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Research Article
According to the nucleotide sequences of the vt1 and vt2 toxin genes in GenBank, four pair of primers were designed and synthesized. vt1 and vt2 were detected in E.coli O157:H7 strains. Bacteriophage was induced and released from the strain only carrying vt2. Phage DNA was extracted and used as PCR ..., Abstract
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Research Article
There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using ..., Abstract
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Brief Reports
Through analyzing the whole cDNA sequence of the sporadic Hepatitis E virus gene and comparing it with other genotypes,we can understand its variability.We used RT-nPCR to assay the HEV RNA of a patient who was diagnosed with sporadic hepatitis E virus. We designed the primers according to ..., Abstract
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Brief Reports
The gene fragment of Avian influenza virus (AIV) was amplified by RT-PCR. The results of sequence analysis showed that high homology(97%) exists among duck, goose and Human influenza virus in Hongkong AIV in 1997, indicating that there are close relationship among these ha genes. The ha gene are ..., Abstract
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Brief Reports
On the basis of genomic sequence of PCV-2 in the GenBank, two primers for amplification of PCV-2 specified fragments were designed and PCR assay for the detection of PCV-2 was established,the kit was used to detect PCV-2. The kit’s sensitivity, specificity and expiration date were tested. The ..., Abstract
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Brief Reports
A 0.5kb antisense RNA fragment was designed targeting the 5’ non-coding region (NCR), translation initiation site and potential transcriptional active region of the sole immediate early gene(IE180) of Pseudorabies virus(PRV). The antisense RNA fragment was amplified by PCR and inserted into the ..., Abstract
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