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Issue of Virologica Sinica
Article List:
Research Article
To develop an attenuated recombinant adenoviral vaccine against Hepatitis C virus infection , we construct the recombinant adenoviral vector expressing non-structural protein 3(NS3) antigen of HCV(RAd-NS3). The HCV NS3 gene enconding for 329-935aa was amplified from the plasmid pRC/NS3 by PCR and ..., Abstract
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Research Article
To identify the pathogen isolated from lung tissue of a patient died from SARS by using electron microscope. Sample of lung tissue taken from autopsy of the SARS patient was inoculated onto Hep2 cultured cells. Any cultured cells exhibiting cytopathic effect were processed by routine techniques for ..., Abstract
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Research Article
Vaginal tract mucosae were the main place of Papillomaviruses replication and sexually transmission. More than 70% of women suffering from cervical intraepithelular neoplasia do not have HPV-specific antibodies in the genital secretion. Therefore, it is very important to develop vaccines, which ..., Abstract
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Research Article
To study the immunological speciality of M protein of SARS-CoV and analyze the feasibility and necessity of M protein as the component of the SARS vaccine. M protein of SARS-CoV was expressed in E.coli by pET-15b and pET-22b and purified by affinity resin as antigen. M gene was cloned into the ..., Abstract
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Research Article
Based on a constructed prokaryotic expression plasmid which carries a ma-jor antigenic segment of E gene of JEV strain SA14-14-2 in our lab, we expressed the gene in methylotrophic yeast Pichia pastoris. The segment was choosn based on an- alysis provided by a computer software because of its ..., Abstract
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Research Article
To study the antiviral activity of Arbidol hydrochloride on Coxsackievirus Group B_3 (CVB_3), the antiviral effect of Arbidol hydrochloride were evaluated with virus inhibitory rates by MTT assay and 50% tissue culture infection dose (TCID_(50)) of the supernatant. The results showed that the viral ..., Abstract
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Research Article
In order to establish a convenient method to detect Porcine Circovirus(pcv2),the ORF2 gene of pcv2 was intergraded into the genome of methylotropic yeast Pichia pastoris to construct an engineering yeast.The ORF2 fragment was successfully secreted into the medium after induced with methanol.Gel ..., Abstract
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Research Article
In order to determine the effect of deletion of gE, gI and TK on the characteristics of virion, we studied the property in cell culture, physicochemical characters and the morphogenesis in vero cells of Pseudorabies virus(PRV) gene-deleted vaccine strain SA215. The results indicated that PRV SA215 ..., Abstract
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Research Article
E0 protein of Classical swine fever virus(CSFV) is of discriminatory feature in serum diagnostic test for developing CSFV Marker vaccine. It is also expected to be useful for monitoring CSFV prevalence,evaluating immunization efficacy and establishing immunization procedure. To obtain a large ..., Abstract
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Research Article
A binary plasmid was constructed by triparental mating, which contained the vp1 gene of foot-and-mouth disease virus strain Akesu/58 serotype O. The Nicotiana Benthamiana (NC) leaves were transformed by Agrobacterium tumefaciens-mediated method with the binary expression plasmid. After selected by ..., Abstract
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Research Article
A transient three-plasmid expression system for the production of pseudotyping virions were used to construct the pseudotype of murine leukemia virus(MuLV) with avian influenza virus hemagglutinin(HA) glycoproteins. Co-transfected pcDNA-HA, pHIT60(include MuLV structural genes, namely Gag and Pol) ..., Abstract
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Research Article
According to mutations occurred in the passages during the Equine infectious anemia virus (EIAV) vaccine attenuation, a full length chimeric gene clone, pLGFD9-12, was constructed successfully at a backbone of clone pLGFD3-8 by substitution with LTR U3 region of a virulent EIAV strain. The ..., Abstract
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Research Article
To construct a new type of vaccine for dog Rabies prevention, Canine adenovirus type-2 was developed for Rabies virus glycoprotein expression. A 1044bp fragment of the E3 region of canine adenovirus type-2 in plasmid pVAX-E3(7.86kb) was deleted by Dra Ⅲ/SspⅠdouble digestion, and an expression ..., Abstract
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Research Article
Simian vacuolating virus 40(SV40)belongs to polyoma virus and is a DNA tumor virus.The natural hosts for SV40 are species of Asian macaque monkeys, especially the rhesus (Maccaca mulatta). SV40 can cause widespread infections leading to acutely pathological changes and long-term virus ..., Abstract
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Research Article
The tissues samples of 828 chickens suspected to be infected by immunodepressive viruses from 42 shicken flocks were collected in Shandong, Henan,Hebei,Beijing,Jiangsu,Guangdong,Guangxi,Sichuan,Jilin,Liaoning and Taiwan,and the DNA was extracted for hybridization in Dot-blot with probes for ..., Abstract
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Research Article
Using suppression subtractive hybridization (SSH) technique, a subtractive cDNA library was constructed from Japanese flounder (Paralichthys olivaceus) embryonic cells (FEC) induced by UV-inactivated dsRNA virus GCHV (Grass carp hemorrhage virus). A housekeeping gene, α-tublin, was used to ..., Abstract
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Research Article
Autographa californica multicapsid nucleopolyhedrovirus(AcMNPV) blocked the Tn-Hi5 cell apoptosis stimulated by Helicoverpa armigera nucleopolyhedrovirus (HaSNPV). Futhermore AcMNPV could rescued HaSNPV replication in Tn-Hi5 cell and produced infective virus. This means that HaSNPV extend the host ..., Abstract
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Research Article
Repeated passage of several fungus-transmitted plant viruses can lead to sequence deletion in their hosts. In this paper, Northern blotting analysis revealed that a low molecular weight component of Wheat yellow mosaic virus(WYMV) RNA1 was detected after the twelfth passage of mechanical ..., Abstract
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Research Article
Golden hamster is one of the favored experimental models for studying mechanisms of transmissible spongiform encephalopathies (TSEs). We used the real-time fluorescent RT-PCR technique, constructing the standard plasmid and curve to quantify the PrP gene expression. Total RNA was isolated from four ..., Abstract
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Research Article
With the reverse transcription chain reaction (RT-PCR), the gene of Canine′s interfe ron-alpha (CaIFN-α) was amplified and cloned , from the total RNA in the lymphocytes stimulated with concanavalin A from the peripheral blood of dog. The amplified fragment was inserted into the prokaryotic ..., Abstract
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Research Article
Ad5-knob gene from AdEasy plasmind DNA was amplified by polymerase chain reaction (PCR) and cloned into an E. coli expression vector pQE30 digested with BamH I and Hind III. After induction with IPTG, the transformed E. coli strain M15 expressed Ad5-knob gene efficiently. Moreover, the recombinant ..., Abstract
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Brief Reports
Based on the reported Big liver and spleen disease virus (BLSV) gene sequence, a pair of primers was designed and a 375bp product was amplified from the total RNA extracted from homogenates of BLSV-infected livers. Twenty liver samples were randomly collected from two commercial broiler breeder ..., Abstract
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Brief Reports
Using two pairs of primer based on conserved sequences of the NP gene of influenza A virus, a RT-nested PCR assay was developed to detect Swine influenza virus in clinical samples. No cross reactions with three other swine pathogens of respiratory disease such as Porcine respiratory and ..., Abstract
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Brief Reports
In this study, the RNA of PRRSV HN1 strain was isolated and the cDNA fragments were amplified by RT-PCR. These fragments were then inserted to T-vector and constructed the cDNA library. The genome of PRRSV HN1 strain was sequenced and submitted to the GenBank obtained the ID: AY457635. The homology ..., Abstract
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Brief Reports
In order to improve the diagnostic efficiency of the hepatitis C in China, the DNA fragments of core, C33c,NS4 in Hepatitis C virus(HCV) were connected and subcloned to pET24a(+). The pET24a-CCN was highly expressed with the inducement IPTG in the E.coli BL21(DE3). In 1L bacterial culture ..., Abstract
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