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Issue of Virologica Sinica
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Research Article
This study was designed to investigate dynamics of cytokines in acute and convalescent patients with Severe acute respiratory syndrome (SARS). The serum levels of cytokines (IL-2, IL-4, IL-8, interleukin-10, IFN-γ, TNF-β and TGF-β) in 95 healthy workers with SARS in our hospital were measured by ..., Abstract
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Research Article
SHIV (SIV/HIV chimeric virus) proviral DNA carrying Human immunodeficiency (virus) type1 (HIV-1 HXBc2) capsid was constructed on the backbone of Simian immunodeficiency (virus) (SIVmm239). The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA. ..., Abstract
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Research Article
In order to clarify the feasibility of adenovirus vector-based multivalent genetic engineering vaccine of Rotavirus (RV), we investigated the immune responses induced by the adenoviruses expressing serotype G2 or G3 VP7 of group A rotavirus on the basis of our previous reports. Balb/c mice were ..., Abstract
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Research Article
Further investigation of this interesting adjuvant-free carrier effect is necessary to ascertain whether peptide-binding portion alone can replace the whole M.tuberculosis Hsp70 acting as carrier. In our study, we selected a portion of E protein with higher index of antigenic determinants dependent ..., Abstract
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Research Article
We reported here that a Ganoderma lucidum polysaccharide (GLP), one of the components extracted and purified from the liquid fermentation of mycelium of Ganoderma lucidum, was active against HSV-1infection in Vero cells. The CC_{50} (50% cytotoxic concentration) value of GLP for Vero cells growth ..., Abstract
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Research Article
In order to evaluate the effect of human neutrophil peptide_{1,3}(HNP_{1,3})and acyclovir (ACV) on Herpes simplex virus type 1 (HSV-1) infection, Vero cells were inoculated with HSV-1. Antiviral activity was evaluated by reduction in cytopathic effect and HSV-1 envelop glycoprotein antigen,which ..., Abstract
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Research Article
RNA were extracted from plasma samples collected from 10 HIV-1 positive patients in Shenyang city, RT-PCR and nest-PCR were carried out , PCR products of HIV-1 gag p17/p24 region were used for sequencing. Sequences of samples were compared with reference sequences of different HIV-1 genetic ..., Abstract
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Research Article
The unspliced and spliced forms of XBP1 stably expressing NS4B in HeLa cells, the transcriptional levels of ATF6, Grp78 and caspase-12, and the luciferase activity in XBP1 and Grp78 promoter reporter assays in HeLa and Huh-7 cells expressing NS4B were detected. The results showed that the two forms ..., Abstract
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Research Article
A recombinant Fowlpox virus (vUTAL3CP1) containing FMDV capsid, P1-2A, and viral 3C protease coding regions,and another plasmid DNA (pVIRIL18P1) encoding P1-2A gene and swine interleukin-18 (IL-18) cDNA,were constructed. guinea pigs were inoculated intramuscularly twice at a 28-day interval with ..., Abstract
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Research Article
A 969bp fragment at the 5′- end of the vp2 gene of Goose parvovirus isolate HG5/82 was subcloned into the Nco I site of prokaryotic expression vector pPROEX~{TM}HTb. The recombinant plasmid was transformed into E.coli DH5α and induced with IPTG. SDS-PAGE analysis showed an induced product band ..., Abstract
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Research Article
Eight full-length cDNAs of H9N2 Avian influenza virus (AIV) genes were amplified and separately cloned into the transcription/expression vector, pHW2000.A total of three genetic tags of silent mutations were introduced into PB2, PB1 and NA genes,respectively. Six 3+5 reassortants were generated by ..., Abstract
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Research Article
Fourteen field strains of J Avian leukosis virus Subgroup(ALV-J)was isolated from farms with suspected diseased chickens and identified by inoculating the samples into embryo fibroblast, indirect immunofluorescence assay (IFA) and PCR amplification To observe the antigenic variants of the GP85 ..., Abstract
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Research Article
3 samples of Duck hepatitis B virus (DHBV) positive serum from Guangdong brown ducks were chosen to be experimental samples. One pair of primers named Q1 and Q2 were designed to amplify the DHBV complete genome. Following the DHBV complete genomes from the 3 DHBV positive serum samples were ..., Abstract
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Research Article
pp38 gene and pp24 gene of MDV Md11 strain were cloned into pBudCE(4.1) vector respectively. The pBudCE(4.1)-pp38 and pBudCE(4.1)-pp24 eukaryotic expressing plasmids were transfected into CEF respectively by lipofectamine reagent. 24, 48, 72 hours after transfection, the expression of pp38 was ..., Abstract
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Research Article
A very virulent strain SD0210 of Infectious bursal disease virus (vvIBDV) was adapted to chicken embryo fibroblast cell (CEF) after propagating in SPF chicken embryo for 10 generations and CEF for 18 generations. The test of pathogenicity, virulent stability and immunogenicity in chickens showed ..., Abstract
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Research Article
Seven pair of primers covering the whole genome of Classical swine fever virus(CSFV) attenuated strain were devised according to the published sequences . 7 cDNA fragments were amplified by RT-PCR from PK-15 cell infected by CSFV attenuated strain. The amplifid fragments were further sequenced ..., Abstract
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Research Article
To test if the foreign gene of a recombinant baculovirus can be transferred to organisms in the same ecological niche, two experiments were performed. First, the fungus Verticillium dahliae Lleb. was cultivated for up to 90 days in laboratory in the presence of BV,ODV and DNA of recombinant ..., Abstract
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Research Article
The cDNA and genomic DNA of ribosomal protein L13 (RpL13) gene were cloned from Helicoverpa zea cells(HzAM1). Sequence analysis indicated that the open reading frame of rpL13 was composed of 666bp without intron,encoding a protein of 25 kDa. The sequence was used to construct phylogentic trees with ..., Abstract
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Research Article
Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass ..., Abstract
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Research Article
Coat protein (CP) gene of Tobacco ringspot virus (TRSV) Shandong isolate 1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR), and was subcloned into the pET-22b(+) prokaryotic expression vector. The recombinant vector was transformed into E.coli strain BL21. Sequence analysis ..., Abstract
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Brief Reports
The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletronmicroscope .It was concluded that the isolate was PCV-2 and was named SCH-A ..., Abstract
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Brief Reports
The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing ..., Abstract
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Research Article
Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed ..., Abstract
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Research Article
Epidemiological characteristics of Hepatitis B virus in population in Xianning of Hubei province were investigated by ELISA, conventional PCR detection as well as specific genotyping system by PCR. Blood specimens were detected by ELISA and conventional PCR, the results indicated that 177(11.1%) of ..., Abstract
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