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Viral macrophage inflammatory protein II(vMIP-II) encoded by Human herpesvirus 8 is a potent antagonist of various chemokine receptors,which are believed to be co-receptors for Human immunodeficiency virus(HIV) entry of different strains.Although it is known as a broad-spectrum HIV entry ..., Abstract
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The 801 base pairs of S1 gene fragment were synthesized based on the Sever acute respiratory syndrome associated coronavirus(SARS-CoV)BJ01 strain registered in GenBank.The synthetical DNA was subcloned into the appropriate site of pcDNA3.1(+) eukariotic expression vector to construct a recombinant ..., Abstract
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The objective of this study was to identify and analyze genotype distribution and genetic variation of Hepatitis E virus(HEV) isolated in Nanjing,China.RT-nPCR based on universal primers for multiple HEV genotypes was used to detect HEV RNA in 40 samples collected from patients with acute sporadic ..., Abstract
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In order to express the recombinant protein of SARS-CoV S receptor-binding region and to study its immunogenicity,the gene encoding SARS-CoV S receptor-binding region was cloned to pET32a+ and expressed in E.coli.The expression of the recombinant protein was confirmed by Western blot analysis.The S ..., Abstract
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To characterize the relationship between exon7 of eNOS gene(position 894G/T) polymorphism and clinical subgroups infected with HBV.The genotype was analyzed by using method of polymerase chain reaction(PCR),enzyme restriction after PCR amplification,and agrose gel electrophresis.Results show that ..., Abstract
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In this work,the truncated Hepatitis C virus(HCV) structural protein E1 fused with GST was expressed in E.coli.The fusion protein was used as an antigen to immunize rabbit to produce the antibody for detection.To express HCV E1 in insect cells,the recombinant baculovirus(vAcHCVE1) containing the ..., Abstract
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PCR method was used to Construct multi-epitope gene ss1c containing HBV S(1-222AA),preS1(10-50AA)and C(1-30AA) gene.The fusion ss1c gene was cloned into the expression vector pPIC3.5k.The recombinant vector was transformed into the host cell of GS115 with electroporation method.After screening with ..., Abstract
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SARS coronavirus(SARS-CoV) is a novel human coronavirus and was responsible for SARS outbreak in several regions of the world in 2003.The genome of SARS-CoV is about 30,000 nucleotides in length and sequence analysis revealed that nucleotides 1-72 contain a RNA leader sequence preceding an ..., Abstract
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The whole cDNA of E gene was amplified by RT-PCR from JEV strain SA14-14,and cloned into the pMD18-T vector.The fragment was identified by restriction enzymes digestion with EcoR I and Xho I,cloned into the pET32a(+) vector.The recombinant plasmid was transformed into BL21 and the recombinant ..., Abstract
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A method of one-step reverse transcription polymerase chain reaction was developed to subtype hemagglutinin(HA) gene of Avian influenza virus(AIV).One pair of primers were designed on the basis of HA gene of AIV,allowing simultaneous detection of AIV subtype H_9 and H_5,and the specific amplicons ..., Abstract
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Five monoclonal antibodies to Infectious bursal disease virus(IBDV),HNF1,HNF7,B34,2B1 and 2G8 were used to screen for binding peptides from peptide 12-mer phage display library.After three rounds of panning(absorption-elution-amplification),sixty positive monoclonal phages(twelve for each ..., Abstract
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The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for ..., Abstract
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A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA ..., Abstract
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The GP3 deleted hydrophobic N-terminal sequence(tGP3) of Porcine reproductive and respiratory syndrome virus(PRRSV) was cloned into prokaryotic expression vector pRSET.The recombinant protein(His)_6-GP3 was highly expressed in E.coli BL21 and could amount to 41% of the total proteins.It could be ..., Abstract
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The full-length 5′-untranslated region(5′UTR) of Porcine endogenous retrovirus(PERV) from Chinese Wuzhi shan(WZS) miniature pigs was cloned by RACE.Seguencing of PERV-WZS 5′UTR showed that it was 1035bp.Compared with other PERV 5’UTR sequences published in GenBank,the homology was ..., Abstract
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The cotyledons and cotyledon petioles of legume forage Lotus corniculatus L.were transformed by Agrobacterium tumefaciens-mediated method with the binary expression vector pBinFMDV-VP1,which contained the vp1gene of FMDV strain Akesu/58 serotype O.Kanamycin-resistant plants were obtained after ..., Abstract
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Primers and probe were designed from the nucleotide sequence within the ORF7 of Porcine reproductive and respiratory syndrome virus(PRRSV),and a quantitative TaqMan real-time PCR was developed for PRRSV detection.Specificity and sensitivity were compared with virus isolation and a conventional ..., Abstract
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A putative v-cathepsin(v-cath) gene of Spodoptera exigua nucleopolyhedrovirus(SeMNPV) was cloned,sequenced and expressed.The results showed that the gene size was 1014 nucleotides,encoding a 338 amino acids putative protein.The amino acid sequence alignment of ten baculoviral v-CATHs showed that ..., Abstract
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The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same ..., Abstract
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Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence ..., Abstract
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Brief Reports
Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and Guangzhou.were detected for TSV with RT-PCR,the ..., Abstract
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Brief Reports
Hepatitis C virus(HCV) H77 strain E1E2 gene DNA vaccine was constructed by inserting full-length cDNA of HCV E1E2 into an eukaryotic expression vector pcDNA4.0.The recombinant plasmid was transfected into eukaryotic cells 293T by calcium phosphate transfection method and transient expressed E1E2 ..., Abstract
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Brief Reports
The gene of nonstructural protein 9 of SARS-coronavirus(SARS-CoV) was amplified using PCR from the product derivated from reverse transcription of SARS-CoV genome RNA,and was inserted into the multiple cloning sites of the expression vector pGEX-6p-1.Recombinant strain induced with IPTG expressed ..., Abstract
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Brief Reports
Several H11N2 subtype of Avian influenza A viruses were isolated from aquatic birds in live bird markets when we surveyed the ecology of the influenza in East China for more than two years and identified by specific RT-PCR.The hemagglutinin(HA) and neuraminidase(NA) genes of one representative ..., Abstract
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