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Issue of Virologica Sinica
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Using hydrodynamic injection. pUC18-HBV1. 3 containing a replication-competent, HBV1.3 supergenomic DNA, was injected into BALB/cJ mice through tail vain. HBV viremia was measured by real-time PCR. Serum concentrations of HBsAg were detected by sandwich ELISA HBeAg was detected and quantified by ..., Abstract
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Hepadnavirus surface, core antigen and c genes and their products which may exist in liver tissues of Marmots himalayana were detected by immunohistochemical staining and in situ hybridization, respectively. The data suggested that hepadnavirus surface antigens were detected in the liver of 13 out ..., Abstract
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A recombinant plasmid named pCI-HBs145 containing a fragment of G145R mutate S gene of Hepatitis B virus was constructed. Seven HeLa cell lines stablely expressing the protein of G145R HBsAg were developed by G418 selection after transfecting with the PCI-HBs145. Biological characteristics of one ..., Abstract
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To investigate the effect of chemokine gene immunization on immune responses induced by HIV-1 envelope protein gene vaccine and to explore new strategies against HIV, Balb/c mice were immunized with pVAX1GP120 alone or co-administered with the DNA encoding for RANTES、and MIP-1α. Their sera were ..., Abstract
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The gene encoding the N protein of SARS virus was cloned and expressed in E. coli. The re-combinant protein was identified by anti-SARS positive sera obtained from recovered SARS patients. The recombinant N protein (55 kDa) reacted specifically with anti-SARS positive sera. The recombinant N ..., Abstract
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Research Article
The etiology of respiratory tract infection (RTI) pathogens, e. g. Human respiratory syncytial virus A and B (RSVA, RSVB), Severe acute respiratory syndrome coronavirus (SARS-Cov), Influenza A virus and Influenza B virus (INFa, INFb), Influenza A virPara-influenza virus 1 and 3 (PIV1、PIV3), M. ..., Abstract
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Research Article
Genes encoding nucleocapsid (N) and envelope (E) proteins of SARS coronavirus (SARS-CoV) were obtained by RT-PCR and were cloned into the expression vector pGEX-KG. The genes were expressed in E. coli and the recombinant proteins were in a soluble form. The proteins were purified by affinity ..., Abstract
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Research Article
Host genetic factors, such as human leukocyte antigen (HLA) alleles, are important in Human immunodeficiency virus ( HIV) infection and its progression to AIDS. HLA class I genes, especially highly polymorphic HLA-B genes, are involved in the activation of HLA-restricted cytotoxic T lymphocytes ..., Abstract
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Research Article
The objective of this study was to determine the relationship between the nucleic acid and antibody of HIV/HCV in a high-risk population. Plasma samples (320) were collected from drug users in Xinjiang and the nucleic acid and antibody of HIV/HCV were detected. A total of 38% of the subjects were ..., Abstract
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Research Article
A multiplex PCR(mPCR) assay was developed and evaluated for its effectiveness as a means to simultaneously detect multiple viral infection of swine . Specific primers for each of the three common DNA viruses, Pseudorabies virus(PRV) , Porcine parvovirus ( PPV) and Porcine circovirus type 2(PCV2) ..., Abstract
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Research Article
Three recombinant fowlpox viruses: rFPV-H5HA-IL18、rFPV-H5HA-H7HA-IL18 and rFPV-H5HA were administered to one-day-old specific pathogen free (SPF) chicken and seven-day-old commercial Leghorn egg laying chicken by wing web route. At 7, 14 and 21 days post infect, hemagglutination inhibition (HI) ..., Abstract
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Research Article
Inhibitory effects of sodium selenite, DL-selenomethionine and Kappa-selenocarrag-eenan on Porcine parvovirus (PPV) infection of the PK-15 cells were studied. The influences of reduced glutathione (GSH) and D-mannitol on antiviral effects of selenium were also carried out in PK-15 cells. The ..., Abstract
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Research Article
To screen out vaccine candidate against PRRSV, adenovirus vector was used to express the M protein of Porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant adenovirus was constructed and the expression of the M protein was identified by RT-PCR and indirect immunofluorescence ..., Abstract
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Research Article
Sandwich ELISA with neutralizing McAbs against rabies virus glycoprotein can be used as a substitutive method to the NIH test. Kunming mice (12-14g) were inoculated ip with different dilution of rabies vaccines two times for NIH test (M-NIH), then challenged with CVS strain of rabies virus. At the ..., Abstract
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Research Article
Two earlier Chinese isolates of bovine viral diarrhea virus , bovine CC-184 and pig ZM-95 were phylogenetically analyzed. The E2 gene encoding a major antigenic was obtained by reverse transcription polymerase chain reaction (RT-PCR) and nest-PCR. Sequencing results showed that the E2 genes of ..., Abstract
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Research Article
A virus isolate from domestic chicken agglutinated chicken erythrocytes and was found as globular enveloped virion of 90nm-100nm diameters under TEM. The isolate was identified as H7N2 Avian influenza virus(AIV) by HI and NI assays and designated as A/Chicken/Hebei/1/ 2002(H7N2,or briefly as ..., Abstract
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Research Article
In order to construct a recombinant canine adenovirus type 2 (CAV-2) of the VP2 of Feline distemper virus or Feline panleukopenia virus (FPV), the vp2 gene fragment of FPV GT-2 strain was amplified by PCR and cloned into pVAX1 vector. The complete VP2 expression cassette was subcloned into the ..., Abstract
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Research Article
A novel flow cytometric assay for evaluating Equine infectious anemia virus (EIAV) antigen-specific CTL responses was established and by using PKH-26 and CFSE as cell colouration dyes. Compared with the traditional 51Cr -release assay, the new method is more sensitive, has a decrease background and ..., Abstract
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Research Article
Eight Spring Viraemia Viruses (SVCV) have been isolated from cultured carp and koi in China between 2002 to 2004. Based on the complete genome of SVCV reference strain, primers were designed and the glycoprotein genes from the 8 isolates were amplified, cloned and se-quenced. These genes were ..., Abstract
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Research Article
Tumor necrosis factor receptor (TNFR) plays an important role in the evasion of immune response by large DNA viruses. TNFR is a homologue of cellular receptors. Lymphocystis disease virus, an iriduvirus, isolated in China (LCDV-C) is a large DNA virus. Primers were designed based on conserved TNFR ..., Abstract
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Research Article
The activity of two isolates of Spotoptera exigua nucleopolyhedrovirus , SeMNPV-M and SeMNPV-Z, was compared by bioassays. The LD50 values of SeMNPV-M and SeMNPV-Z in 3rd instar host larvae were 195. 8 PIBs/ gram diet and 242. 4 PIBs/gram diet, respectively. The LT50 values infected with ..., Abstract
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Research Article
Primers based on published sequences of granulin genes were synthesized and used to amplify the granulin gene from Clostera anachoreta granulovirus (ClanGV). The generated product was se-quenced. Sequence analysis indicated the entire ORF of ClanGV granulin gene was 747bp, encoding a protein of 248 ..., Abstract
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Research Article
The major pathological changes of Pieris rapae infected by a new nodavirus (PrNV) and a mixture of PrNV and Pieris rapae Granulosis virus (PiraGV) were studied by electron microscopy and histochemisty. The results showed that PrNV replicated only in the cytoplasm of larval midgut cells. After ..., Abstract
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Research Article
根据GenBank中VT1、VT2毒素的基因序列设计合成2对引物,以大肠杆菌O157:H7菌株DNA为模板,扩 增vt1、vt2。诱导只扩增出vt2的菌株释放噬菌体,利用多种指示菌经双层琼脂平板法来分离纯化VT2噬菌体,观 察噬菌斑的特征,提纯病毒粒子进行电镜观察,并对噬菌体中vt2基因检测、克隆和序列分析。结果显示VT2噬菌 ..., Abstract
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Brief Reports
The development of effective strategies against cervical cancer require knowledge on the prevalence and molecular biology of the Human papillomavirus (HPV). We have investigated HPV infection in 60 women suffering from cervical cancer. HPV was typed by PCR of DNA extracts. The prevalence of ..., Abstract
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