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In order to elucidate the relationship between genetic characteristics and measles outbreak,and the influence of antigenic variation on measles vaccine effect,we investigated the genetic characteristics and antigenic variation of measles viruses isolated in Zhejiang province during 1999~ 2003.Rats ..., Abstract
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Research Article
The extracellular portion of glycoprotein D gene(gDt),encoding 1-314 amino acids,was amplified from extracted HSV-1 DNA and cloned into expression vector pPIC9K.After electroporation transformation of Pichia pastoris GS115 and selection with G418,a strain of P.pastoris with high yield of secreted ..., Abstract
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Recombinant fusion prokaryotic expression vector pGEX-4T-1/hsp70-S was constructed by cloning genes Hsp70 and Hantaan virus S gene coding region into pGEX-4T and was expressed in E. coli. The Hsp70-NP fusion protein was separated and purified with GSTrapFF purification system. We ..., Abstract
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Research Article
To investigate the action and mechanisms of Epstein Barr-virus(EBV) latent membrane protein1(LMP1) in stimulating the proliferation of primary mouse embryonic fibroblast (MEF) cells the retrovirus (RV) containing wild type LMP1(wt-LMP1) and mutant type LMP1(mt-LMP1);which ..., Abstract
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Research Article
The suppression subtractive hybridization (SSH) and bioinformatics techniques were used for screening and cloning of the target genes down-regulated byβ-interferon in HepG2. The mRNA was isolated from HepG2 cells transfected pcDNA3.1(-)IFNβand pcDNA3.1(-) empty vector ..., Abstract
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Research Article
A synthesized multiple-mimotope gene of Hepatitis C virus was cloned into a prokaryotic expression vector pGEX-4T-1 to generate a fusion protein GST-HCVME. The reactivity of the GST- HCVME with the sera of HCV patients was measured by Western-blot and ELISA. BALB/c mice were ..., Abstract
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Research Article
Porcine circovirus 2 (PCV2) has been implicated as the etiological agent of a new disease, named postweaning multisystemic wasting syndrome (PMWS). PCV2 contains two major open reading frames (ORFs): ORF1 and ORF2, encoding replication-associated (Rep) protein and capsid (Cap) ..., Abstract
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Research Article
Avian reticuloendotheliosis virus (REV) infection was reported to be very common in chicken flocks in China, but its economic impact on the poultry industry was not clear. The results in this study indicated that REV infection in 1-day-old SPF chickens could severely ..., Abstract
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Research Article
The intercellular adhension molecular-1(ICAM-1)and it’s its receptors in the mice’s lungs of HPAIV and control mice pneumonia were detected by using immunohistochemistry staining after infected mice with Highly pathogenic avian influenza virus (HPAIV). The expressive level of ..., Abstract
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Research Article
A pair of clone primers were designed and synthesized based on neuraminidase(NA) gene sequences of known Avian influenza virus H5N1 subtypes. NA gene were amplified from total RNA, which had been extracted from allantoic fluid of H5N1 subtype virus inoculated embryo, by reverse ..., Abstract
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Research Article
The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes ..., Abstract
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Research Article
To express the polyhedrin (the tenth segment, S10) of Dendrolimus punctatus Cypovirus (DpCPV) and investigate the localization of the polyhedrin in eukaryote, The polyhedrin gene was amplified from DpCPV by RT-PCR and cloned into pET-28a vector. The polyhedrin was then expressed ..., Abstract
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Research Article
The gp41 gene was cloned from Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) Japan-C3 strain. This gene is 993bp long and encodes a polypeptide with a predicted molecular mass of 36.9 kDa. Analysis showed the gp41of SpltMNPV Japan-C3 strain was most closely related ..., Abstract
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Research Article
A virus isolated from cattleya in Xiamen, China was identified as an isolate of Odontoglossum ringspot virus, based on its biological properties, particle morphology, serological characterization and RT-PCR. Mechanical inoculation test with the isolate was carried out to 27 species of 6 families of ..., Abstract
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Brief Reports
The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised ..., Abstract
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Brief Reports
The LacZ gene controlled under the Marek’s disease virus (MDV) glycoprotein B (gB) promoter was excised from plasmid pSK-gB-LacZ and inserted into US10 gene. Two plasmids were constructed, pUS-gB-LacZ(L)and pUS-gB-LacZ(U)),which differ with regard to the orientation of the expression cassette. ..., Abstract
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Brief Reports
The Erns gene was amplified by RT-nPCR from C-strain of Classical swine fever virus (CSFV). Then the Erns gene was cloned into pGEX6P-1 vector. The expression products were analysed by SDS-PAGE and Western blotting methods. The inclusion bodies were recovered from bacterial ..., Abstract
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Brief Reports
Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the Small Envelope (E) gene of avian Infectious bronchitis virus (IBV) LX4 strain and the gene was cloned into the pMD18-T vector. By digestion with restriction enzymes SalⅠand BamHⅠ, the E gene was subcloned into ..., Abstract
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Brief Reports
In order to develop an assay to distinguish the infection of Equine infectious anemia virus (EIAV) American strains from Donkey-leukocyte attenuated virus (DLV) strain, eight primers were designed based on the comparison of complete sequences of four EIAV American strains and DLV. ..., Abstract
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